Research areas
I am interested in the structure and function of eukaryotic carbohydrate processing enzymes. Obtaining a greater appreciation of how these enzymes assimilate or degrade carbohydrates is important in understanding the biological roles that carbohydrates play in cellular processes, and also how these processes malfunction in a number of diseases, such as cancer, lysosomal storage disorders, and neurodegenerative diseases.
I am currently investigating the enzymes responsible for the degradation of heparan sulphate, a component of the extracellular matrix. Using a combination of approaches, including molecular biology, protein production, X-ray crystallography, enzyme kinetics and cell culture, I aim to gain a greater understanding of these enzymes from a mechanistic and structural perspective. Hopefully this will lead to the development of tools, such as enzyme inhibitors, that can be used to probe the biological functions of the enzymes, and possibly also for therapeutic applications in the longer term.
PhD supervision
- Catriona Haberland
- Emma Hobbs
- Anna Zolotariof
- Di Zhu
- Brendon Medley
- Shannon Richardson
- Peter Black
- Stuart McQuarrie
- Misha Atkinson
Selected publications
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Open access
Activation of Csm6 ribonuclease by cyclic nucleotide binding: in an emergency, twist to open
McQuarrie, S., Athukoralage, J. S., McMahon, S. A., Graham, S., Ackermann, K., Bode, B. E., White, M. F. & Gloster, T. M., 27 Oct 2023, In: Nucleic Acids Research. 51, 19, p. 10590-10605 16 p.Research output: Contribution to journal › Article › peer-review
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Open access
CRISPR antiphage defence mediated by the cyclic nucleotide-binding membrane protein Csx23
Grüschow, S., McQuarrie, S., Ackermann, K., McMahon, S., Bode, BE., Gloster, TM. & White, MF., 13 Mar 2024, (E-pub ahead of print) In: Nucleic Acids Research. 15 p., gkae167.Research output: Contribution to journal › Article › peer-review
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Open access
Activation of Csm6 ribonuclease by cyclic nucleotide binding: in an emergency, twist to open
McQuarrie, S., Athukoralage, J. S., McMahon, S. A., Graham, S., Ackermann, K., Bode, B. E., White, M. F. & Gloster, T. M., 27 Oct 2023, In: Nucleic Acids Research. 51, 19, p. 10590-10605 16 p.Research output: Contribution to journal › Article › peer-review
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Open access
cazy_webscraper: local compilation and interrogation of comprehensive CAZyme datasets
Hobbs, E. E. M., Gloster, T. & Pritchard, L., 14 Aug 2023, In: Microbial Genomics. 9, 8, 23 p., 001086.Research output: Contribution to journal › Article › peer-review
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Open access
Structure, dynamics, and molecular inhibition of the Staphylococcus aureus m1A22-tRNA methyltransferase TrmK
Sweeney, P., Galliford, A., Kumar, A., Raju, D., Krishna, N. B., Sutherland, E., Leo, C. J., Fisher, G., Lalitha, R., Muthuraj, L., Sigamani, G., Oehler, V., Synowsky, S. A., Shirran, S. L., Gloster, T., Melo Czekster, C., Kumar, P. & da Silva, R. G., May 2022, In: Journal of Biological Chemistry. 298, 6, 15 p., 102040.Research output: Contribution to journal › Article › peer-review
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Open access
cazy_webscraper: local compilation and interrogation of comprehensive CAZyme datasets
Hobbs, E. E. M., Gloster, T. & Pritchard, L., 4 Dec 2022, 19 p. (biorxiv).Research output: Working paper › Preprint
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Open access
Comprehensive evaluation of CAZyme prediction tools in fungal and bacterial species
Hobbs, E. E. M., Gloster, T., Chapman, S. & Pritchard, L., 26 Apr 2021.Research output: Contribution to conference › Poster
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Kinetic and structural characterization of sialidases (Kdnases) from ascomycete fungal pathogens
Nejatie, A., Steves, E., Gauthier, N., Baker, J., Nesbitt, J., McMahon, S. A., Oehler, V., Thornton, N. J., Noyovitz, B., Khazaei, K., Byers, B. W., Zandberg, W. F., Gloster, T. M., Moore, M. M. & Bennet, A. J., 19 Nov 2021, In: ACS Chemical Biology. 16, 11, p. 2632–2640 9 p.Research output: Contribution to journal › Article › peer-review
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Open access
Sialidase and sialyltransferase inhibitors: targeting pathogenicity and disease
Bowles, W. H. & Gloster, T., 29 Jul 2021, In: Frontiers in Molecular Biosciences. 8, 10 p., 705133.Research output: Contribution to journal › Review article › peer-review
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Open access
The CRISPR ancillary effector Can2 is a dual-specificity nuclease potentiating type III CRISPR defence
Zhu, W., McQuarrie, S. J., Gruschow, S., McMahon, S., Graham, S., Gloster, T. & White, M., 18 Mar 2021, In: Nucleic Acids Research. 49, 5, p. 2777–2789 13 p., gkab073.Research output: Contribution to journal › Article › peer-review