FMRG Searchable EST Database

• Library: Tiger pufferfish (Takifugu rubripes) Subtracted cDNA libraries
  Constructed by: J. M. O. Fernandes and Mathew Mackenzie
  Source material: Fast myotomal muscle
  Vector: pCR4-TOPO (Invitrogen)
  Collaborators: J. R. Kinghorn, Shugo Watabe, Yuzuru Sukuki and Greg Elgar
  • ESTs Forward subtracted library (Myotube -) cDNA from a 3.4 kg fish as tester and cDNA from a 180g fish as driver
  • ESTs Reverse subtracted library (Myotube +) cDNA from a 180g fish as tester and cDNA from a 3.4 kg fish as driver
• Library: Developmental stages of Atlantic halibut (Hippoglossus hippoglossus) cDNA libraries
  Constructed: by Jai-Lin Bai
  Source material: Fast myotomal muscle
  Vector: pBlueScript II SK+ XR (Stratagene)
  Collaborators: Joint project with Christel Solberg, Bodø University College, Norway
  • ESTs 1 day-old Yolk-sac larvae dissected trunks
  • ESTs Fast myotomal muscle farmed juvenile 420 g
  • ESTs 2 cell embryos
• Library: King scallop (Pecten maximus) cDNA libraries
  Constructed: by Helene Pagander & Hannah Chubb
  Source material: Striated adductor muscle
  Vector: pBlueScript II (SK+) XR (Stratagene)
  Collaborators: Joint project with Øivind Andersen, AQUAFORSK, Aas, Norway
  • King Scallop cDNA library
  • Reverse subtracted cDNA library from King scallop

• Library: Atlantic salmon (Salmo salar) subtracted cDNA library
  Constructed by: J. M. O. Fernandes and S. Consuegra
  Source material: Fast myotomal muscle
  Vector: pCR4-TOPO (Invitrogen)
  
  • ESTs Reverse subtracted library (myotube -) cDNA from a 4.5 kg fish as tester and cDNA from a 650 g fish as driver
  • ESTs Forward subtracted library (myotube +); cDNA from a 650g fish as tester and cDNA from a 4.5 kg fish as driver

Sequence traces were analysed using the EST pipeline developed by the NERC EGTDC