Research areas
Measuring nanometre distances for structural biology using EPR spectroscopy.
I use Electron Paramagnetic Resonance (EPR) spectroscopy to measure nanometre distances in proteins and nucleic acids. This provides detail on their structure and dynamics. The methodology is applied to a range of proteins including those for protein excretion in the cell. I am also interested in developing the methods for tagging proteins and nucleic acids to make them suitable for this kind of investigation, as well as advancing the EPR experiments themselves.
The biological preparation and EPR facilities at St Andrews are outstanding with, among others, pulsed and CW X-band, high power Q-band and the home-built high power W-band (HiPER).
PhD supervision
- Michael Taylor
- Hannah Russell
- Elena Mocanu
Selected publications
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Triggered functional dynamics of AsLOV2 by time-resolved electron paramagnetic resonance at high magnetic fields
Maity, S., Price, B. D., Wilson, C. B., Mukherjee, A., Starck, M., Parker, D., Wilson, M. Z., Lovett, J. E., Han, S. & Sherwin, M. S., 14 Feb 2023, (E-pub ahead of print) In: Angewandte Chemie International Edition. Early View, 9 p., e202212832.Research output: Contribution to journal › Article › peer-review
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Open access
Combined pulsed electron double resonance EPR and molecular dynamics investigations of calmodulin suggest effects of crowding agents on protein structures
Stewart, A., Shanmugam, M., Kutta, R., Scrutton, N., Lovett, J. E. & Hay, S., 6 Sep 2022, In: Biochemistry. 61, 17, p. 1735–1742 8 p.Research output: Contribution to journal › Article › peer-review
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Open access
DEER data analysis software: a comparative guide
Russell, H., Cura, R. & Lovett, J. E., 1 Jun 2022, In: Frontiers in Molecular Biosciences. 9, 17 p., 915167.Research output: Contribution to journal › Article › peer-review
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Open access
Neural networks in pulsed dipolar spectroscopy: a practical guide
Keeley, J., Choudhury, T., Galazzo, L., Bordignon, E., Feintuch, A., Goldfarb, D., Russell, H., Taylor, M. J., Lovett, J. E., Eggeling, A., Fabregas Ibanez, L., Keller, K., Yulikov, M., Jeschke, G. & Kuprov, I., May 2022, In: Journal of Magnetic Resonance. 338, 14 p., 107186.Research output: Contribution to journal › Article › peer-review
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Open access
Benchmark test and guidelines for DEER/PELDOR experiments on nitroxide-labeled biomolecules
Schiemann, O., Heubach, C., Abdullin, D., Ackermann, K., Azarkh, M., Bagryanskaya, E., Drescher, M., Endeward, B., Freed, J., Galazzo, L., Goldfarb, D., Hett, T., Esteban Hofer, L., Fábregas Ibáñez, L., Hustedt, E., Kucher, S., Kuprov, I., Lovett, J. E., Meyer, A., Ruthstein, S., Saxena, S., Stoll, S., Timmel, C., Di Valentin, M., Mchaourab, H., Prisner, T., Bode, B. E., Bordignon, E., Bennati, M. & Jeschke, G., 19 Oct 2021, (E-pub ahead of print) In: Journal of the American Chemical Society. Articles ASAP, 16 p.Research output: Contribution to journal › Article › peer-review
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Open access
DEER and RIDME measurements of the nitroxide-spin labelled copper-bound amine oxidase homodimer from arthrobacter globiformis
Russell, H., Stewart, R., Prior, C., Oganesyan, V. S., Gaule, T. G. & Lovett, J. E., 29 Mar 2021, (E-pub ahead of print) In: Applied Magnetic Resonance. First Online, 21 p.Research output: Contribution to journal › Article › peer-review
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Open access
Versatile para-substituted pyridine lanthanide coordination complexes allow late stage tailoring of complex function
Starck, M., Fradgley, J., De Rosa, D. F., Batsanov, A., Papa, M., Taylor, M., Lovett, J., Lutter, J., Allen, M. & Parker, D., 16 Nov 2021, (E-pub ahead of print) In: Chemistry - A European Journal. Early View, 7 p.Research output: Contribution to journal › Article › peer-review
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Open access
2'-Alkynyl spin-labelling is a minimally perturbing tool for DNA structural analysis
Hardwick, J., Haugland, M., El-Sagheer, A., Ptchelkine, D., Beierlein, F., Lane, A., Brown, T., Lovett, J. E. & Anderson, E., 13 Feb 2020, (E-pub ahead of print) In: Nucleic Acids Research. Advance articles, gkaa086.Research output: Contribution to journal › Article › peer-review
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Open access
High-sensitivity Gd3+-Gd3+ EPR distance measurements that eliminate artefacts seen at short distances
El Mkami, H., Hunter, R. I., Cruickshank, P. A. S., Taylor, M. J., Lovett, J. E., Feintuch, A., Qi, M., Godt, A. & Smith, G. M., 9 Dec 2020, In: Magnetic Resonance. 1, 2, p. 301-313Research output: Contribution to journal › Article › peer-review
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Open access
Iron is a ligand of SecA-like metal-binding domains in vivo
Cranford-Smith, T., Jamshad, M., Jeeves, M., Chandler, R. A., Yule, J., Robinson, A., Alam, F., Dunne, K. A., Aponte Angarita, E. H., Alanazi, M., Carter, C., Henderson, I. R., Lovett, J. E., Winn, P., Knowles, T. & Huber, D., 2 Apr 2020, (E-pub ahead of print) In: Journal of Biological Chemistry. Papers in PressResearch output: Contribution to journal › Article › peer-review