Research areas
The kidneys play a critical role in maintaining electrolyte homeostasis within the body. The regulation of sodium balance in particular maintains our circulating volume within narrow parameters and importantly contributes to the long-term control of blood pressure. Transport of electrolytes occurs via a number of different ion channels and transporters expressed at the surface of the epithelial cells lining the tubules of the nephron. Within the distal nephron, these processes are under strict regulation by a plethora of hormones and bioactive factors which can rapidly alter ion transport in response to physiological and pathophysiological challenges. Dysfunction of these transport processes and their regulation give rise to electrolyte disorders as well as dysregulated arterial blood pressure.
Our lab focuses on understanding the molecular mechanisms which underpin hormonal control of epithelial ion transport in the distal nephron in both health and disease. We combine electrophysiological methods with cutting edge molecular techniques to investigate receptor binding and translocation; intracellular signalling cascades; as well as subsequent modulation of ion transport, with a particular focus on Na+ transport via the epithelial sodium channel (ENaC).
PhD supervision
- Struan Loughlin
- Morag Milne
- Etang Collins Etang
Selected publications
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Open access
Ion channels and transporters keep ideas flowing
Mansley, M. K. & Fong, P., 1 May 2023, In: The Journal of Physiology. 601, 9, p. 1517-1519 3 p.Research output: Contribution to journal › Editorial › peer-review
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Open access
Mapping the transcriptome underpinning acute corticosteroid action within the cortical collecting duct
Loughlin, S. R., Costello, H. M., Roe, A. J., Buckley, C., Wilson, S. M., Bailey, M. A. & Mansley, M. K., 24 Nov 2022, In: Kidney360. 3, 11, 40 p.Research output: Contribution to journal › Article › peer-review
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Open access
Prostaglandin E2 stimulates the epithelial sodium channel (ENaC) in cultured mouse cortical collecting duct cells in an autocrine manner
Mansley, M. K., Niklas, C., Nacken, R., Mandery, K., Glaeser, H., Fromm, M. F., Korbmacher, C. & Bertog, M., 22 May 2020, In: Journal of General Physiology. 152, 8, 15 p., e201912525.Research output: Contribution to journal › Article › peer-review
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Open access
Trichostatin A blocks aldosterone-induced Na+ transport and control of serum- and glucocorticoid-inducible kinase 1 in cortical collecting duct cells
Mansley, M. K., Roe, A. J., Francis, S. L., Gill, J. H., Bailey, M. A. & Wilson, S. M., 16 Jan 2020, In: British Journal of Pharmacology. 176, 24, p. 4708-4719 12 p.Research output: Contribution to journal › Article › peer-review
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Open access
Glucocorticoid receptor activation stimulates the sodium-chloride cotransporter and influences the diurnal rhythm of its phosphorylation
Ivy, J. R., Jones, N. K., Costello, H. M., Mansley, M. K., Peltz, T. S., Flatman, P. W. & Bailey, M. A., 2019, In: American Journal of Physiology - Renal Physiology. 317, 6, p. F1536-F1548Research output: Contribution to journal › Article › peer-review
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Inhibitors of the proteasome stimulate the epithelial sodium channel (ENaC) through SGK1 and mimic the effect of aldosterone
Mansley, M. K., Korbmacher, C. & Bertog, M., Feb 2018, In: Pflugers Archiv European Journal of Physiology. 470, 2, p. 295-304 10 p.Research output: Contribution to journal › Article › peer-review
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Open access
mCCD cl1 cells show plasticity consistent with the ability to transition between principal and intercalated cells
Assmus, A. M., Mansley, M. K., Mullins, L. J., Peter, A. & Mullins, J. J., 1 Jan 2018, In: American Journal of Physiology - Renal Physiology. 314, 5, p. F820-F831Research output: Contribution to journal › Article › peer-review
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Activation of the Epithelial Sodium Channel (ENaC) by Aldosterone in mCCDcl1 Mouse Renal Cortical Collecting Duct Cells is Dependent on SGK1 and Can be Prevented by Inhibiting Endogenous Proteases: Experimental Biology 2017
Mansley, M., Korbmacher, C. & Bertog, M., Apr 2017.Research output: Contribution to conference › Paper › peer-review
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Aldosterone-induced Na+ Absorption is Regulated by Protein Acetylation in a Cellular Model of the Cortical Collecting Duct: Experimental Biology 2017
Mansley, M., Roe, A., Francis, S., Gill, J., Bailey, M. & Wilson, S., Apr 2017.Research output: Contribution to conference › Paper › peer-review
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Characterisation of a Mouse Kidney Cortical Collecting Duct Cell Line Suggests Plasticity Between Cell Types: Experimental Biology 2017
Assmus, A., Mansley, M., Mullins, L., Peter, A. & Mullins, J., Apr 2017.Research output: Contribution to conference › Paper › peer-review