Dr Tracey Gloster

Reader

Researcher profile

Phone

+44 (0)1334 467245

Email

tmg@st-andrews.ac.uk

Research areas

I am interested in the structure and function of eukaryotic carbohydrate processing enzymes. Obtaining a greater appreciation of how these enzymes assimilate or degrade carbohydrates is important in understanding the biological roles that carbohydrates play in cellular processes, and also how these processes malfunction in a number of diseases, such as cancer, lysosomal storage disorders, and neurodegenerative diseases.

I am currently investigating the enzymes responsible for the degradation of heparan sulphate, a component of the extracellular matrix. Using a combination of approaches, including molecular biology, protein production, X-ray crystallography, enzyme kinetics and cell culture, I aim to gain a greater understanding of these enzymes from a mechanistic and structural perspective. Hopefully this will lead to the development of tools, such as enzyme inhibitors, that can be used to probe the biological functions of the enzymes, and possibly also for therapeutic applications in the longer term.

PhD supervision

• Emma Hobbs
• Teresa Filipa Guerreiro Machado
• Agnieszka Bogucka
• Flora Foltanyi
• Catriona Haberland

Selected publications

• An anti-CRISPR viral ring nuclease subverts type III CRISPR immunity

  Athukoralage, J. S., McMahon, S., Zhang, C., Gruschow, S., Graham, S., Krupovic, M., Whitaker, R., Gloster, T. & White, M., 23 Jan 2020, In : Nature. 577, 7791, p. 572-575 19 p.

  Research output: Contribution to journal ? Article

• Exploitation of carbohydrate processing enzymes in biocatalysis

  Gloster, T. M., Apr 2020, In : Current Opinion in Chemical Biology. 55, p. 180-188 9 p.

  Research output: Contribution to journal ? Review article

• Structure and mechanism of a Type III CRISPR defence DNA nuclease activated by cyclic oligoadenylate

  McMahon, S., Zhu, W., Graham, S., Rambo, R., White, M. & Gloster, T., 24 Jan 2020, In : Nature Communications. 11, 11 p., 500.

  Research output: Contribution to journal ? Article

• Tetramerisation of the CRISPR ring nuclease Crn3/Csx3 facilitates cyclic oligoadenylate cleavage

  Athukoralage, J. S., McQuarrie, S. J., Gruschow, S., Graham, S., Gloster, T. & White, M., 28 Jun 2020, (Accepted/In press) In : eLife. In press

  Research output: Contribution to journal ? Article

• Linear Eyring plots conceal a change in rate-limiting step in an enzyme reaction

  Machado, T. F. G., Gloster, T. & da Silva, R. G., 11 Dec 2018, In : Biochemistry. 57, 49, p. 6757-6761

  Research output: Contribution to journal ? Article

• Metabolic inhibitors of O-GlcNAc transferase (OGT) that act in vivo implicate decreased O-GlcNAc levels in leptin-mediated nutrient sensing

  Liu, T-W., Zandberg, W. F., Gloster, T. M., Deng, L., Murray, K. D., Shan, X. & Vocadlo, D. J., 19 Jun 2018, In : Angewandte Chemie International Edition. 57, 26, p. 7644-7648 5 p.

  Research output: Contribution to journal ? Article

• New irreversible ?-?-iduronidase inhibitors and activity-based probes

  Artola, M., Kuo, C-L., McMahon, S. A., Oehler, V., Hansen, T., van der Lienden, M., He, X., van den Elst, H., Florea, B. I., Kermode, A. R., van der Marel, G. A., Gloster, T. M., Codée, J. D. C., Overkleeft, H. S. & Aerts, J. M. F. G., 26 Nov 2018, In : Chemistry - A European Journal. Early View, 9 p.

  Research output: Contribution to journal ? Article

• Revealing the mechanism for covalent inhibition of glycoside hydrolases by carbasugars at an atomic level

  Ren, W., Pengelly, R. J., Farren-Dai, M., Abadi, S. S. K., Oehler, V., Akintola, O., Draper, J., Meanwell, M., Chakladar, S., ?widerek, K., Moliner, V., Britton, R., Gloster, T. & Bennet, A., 13 Aug 2018, In : Nature Communications. 9, 12 p., 3243.

  Research output: Contribution to journal ? Article

• The enzymatic degradation of heparan sulfate

  Griffin, L. S. & Gloster, T. M., 1 Aug 2017, In : Protein & Peptide Letters. 24, 8, p. 710-722

  Research output: Contribution to journal ? Review article

• Mechanism of the human nucleocytoplasmic hexosaminidase D

  Alteen, M. G., Oehler, V., Nem?ovi?ová, I., Wilson, I. B. H., Vacadlo, D. J. & Gloster, T. M., 17 May 2016, In : Biochemistry. 55, 19, p. 2753-2747

  Research output: Contribution to journal ? Article

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