Dr Sally Shirran
Senior Scientific Officer
Research areas
I manage the BSRC mass spectrometry core facility, which supports the research of many of the researchers within the Biomedical Sciences Research Complex, the University of St Andrews, and many other Universities in the UK. I am the first point of contact for collaborations, and will advise on experiment design and assist with interpretation of results.
The facility is mainly Proteomics based, specialising in 2 main areas:
Protein Identification - From simple gel band ID to identification of all proteins in a complex pullodwn mixture. Confirmation of post-translational modifications, spin labelling, chemical crosslinks, point mutations, truncations, all aspects of protein identification are supported. Measurement of intact protein mass is also undertaken, including membrane proteins.
Protein Quantitation - Relative protein quantitation by both iTRAQ and SWATH. Global protein quantitation upon exposure to stress.
PhD supervision
- Debra Lennox
- Hasnain Ahmed
Selected publications
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Open access
Characterising the HLA-I Immunopeptidome of plasma-derived extracellular vesicles in patients with melanoma
Boyne, C. M., Coote, A., Synowsky, S. A., Naden, A. B., Shirran, S. L. & Powis, S. J., Mar 2024, In: Journal of Extracellular Biology. 3, 3, 12 p., e146.Research output: Contribution to journal › Article › peer-review
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Open access
Reversible conjugation of a CBASS nucleotide cyclase regulates bacterial immune response to phage infection
Kruger, L., Gaskell-Mew, L., Graham, S., Shirran, S. L., Hertel, R. & White, M., 8 Apr 2024, In: Nature Microbiology.Research output: Contribution to journal › Article › peer-review
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A Discussion of Core Facility Biomarker Discovery
Shirran, S. L., 3 Nov 2023, p. 8. 1 p.Research output: Contribution to conference › Other
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Open access
AAV9-mediated SMN gene therapy rescues cardiac desmin but not Lamin A/C and elastin dysregulation in Smn2B/- spinal muscular atrophy mice
Brown, S. J., Šoltić, D., Synowsky, S. A., Shirran, S. L., Chilcott, E., Shorrock, H. K., Gillingwater, T. H., Yáñez-Muñoz, R. J., Schneider, B., Bowerman, M. & Fuller, H. R., 15 Oct 2023, In: Human Molecular Genetics. 32, 20, p. 2950-2965 16 p.Research output: Contribution to journal › Article › peer-review
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Open access
Antiviral type III CRISPR signalling via conjugation of ATP and SAM
Chi, H., Hoikkala, V. P., Gruschow, S., Graham, S., Shirran, S. L. & White, M., 26 Oct 2023, In: Nature. 622, 7984, p. 826-833 22 p.Research output: Contribution to journal › Article › peer-review
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Open access
Assessing the impact of interfering organic matter on soil metaproteomic workflow
Waibel, M., McDonnell, K., Tuohy, M., Shirran, S., Synowsky, S., Thornton, B., Paterson, E., Brennan, F. & Abram, F., 22 Jun 2023, In: European Journal of Soil Science. 74, 3, 14 p., e13392.Research output: Contribution to journal › Article › peer-review
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Open access
Unusual peptide-binding proteins guide pyrroloindoline alkaloid formation in crocagin biosynthesis
Adam, S., Zheng, D., Klein, A., Volz, C., Mullen, W., Shirran, S. L., Smith, B. O., Kalinina, O. V., Müller, R. & Koehnke, J., 1 Apr 2023, In: Nature Chemistry. 15, 4, p. 560-568 9 p.Research output: Contribution to journal › Article › peer-review
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Open access
Carbapenem-only combination therapy against multi-drug resistant Pseudomonas aeruginosa: assessment of in vitro and in vivo efficacy and mode of action
Mackay, B., Parcell, B. J., Shirran, S. L. & Coote, P. J., 25 Oct 2022, In: Antibiotics. 11, 11, 19 p., 1467.Research output: Contribution to journal › Article › peer-review
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Open access
Identification of plasma proteins associated with oesophageal cancer chemotherapeutic treatment outcomes using SWATH-MS
Guo, N., Minas, G., Synowsky, S. A., Dunne, M. R., Ahmed, H., McShane, R., Bhardwaj, A., Donlon, N. E., Lorton, C., O'Sullivan, J., Reynolds, J. V., Caie, P. D., Shirran, S. L., Lynch, A., Stewart, A. J. & Arya, S., 30 Aug 2022, In: Journal of Proteomics. 266, 11 p., 104684.Research output: Contribution to journal › Article › peer-review
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Open access
Structure, dynamics, and molecular inhibition of the Staphylococcus aureus m1A22-tRNA methyltransferase TrmK
Sweeney, P., Galliford, A., Kumar, A., Raju, D., Krishna, N. B., Sutherland, E., Leo, C. J., Fisher, G., Lalitha, R., Muthuraj, L., Sigamani, G., Oehler, V., Synowsky, S. A., Shirran, S. L., Gloster, T., Melo Czekster, C., Kumar, P. & da Silva, R. G., May 2022, In: Journal of Biological Chemistry. 298, 6, 15 p., 102040.Research output: Contribution to journal › Article › peer-review