Single-molecule fluorescence imaging techniques
Currently, we are implementing a range of single-molecule techniques in our lab, mostly to work with immobilised biomolecules: Single-molecule Total-Internal Reflection (both prism- and objective type based, see figure a and b) and single-molecule confocal (figure c) for those faster biological process that need high collection rates.
(a) Sm-TIR: prism-type |
(b) Sm-TIR: objective-type |
(c) Sm-confocal |
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