Principal Investigator: Prof. Carlos Penedo


The twists and turns of organic semiconductor chains: a single-molecule view of conjugated polymers in solution   
A. Brenlla-Lopez,  Ifor D. W. Samuel and J. Carlos Penedo*. In preparation            
    2023  
TWIN-FRET: overcoming the orthogonal chemistry labelling barrier using equilibrium-encoded FRET pairs
S. Jana, M. Diez-Castellnou, S. Thomson,  M. Fossepre, M. Surin, Y. Olivier, E. Kay, J. Carlos Penedo* In preparation 

    2023  
Energy transfer from end-terminal labelled polyfluorene molecules discriminates alpha and beta-phase content
Alfonso Brenlla-Lopez,  Ifor D W Samuel J. Carlos Penedo To be submitted  

    2023  
Zuo1 preferentially induces and stabilizes unstable G4 structures
Ines Burkhart,  Michaela Limmer, J. Carlos Penedo, Harald Schwalbe and Katrin Paeschke To be submitted, Nat. Comm.  

    2023  
Topographic fingerprinting of single proteins and proteoforms
J. Carlos Penedo Nat. Nanotech, In revision 

    2023  
Cotranscriptional folding of a 5' stem-loop in the Escherichia Coli tbpA riboswitch at single-nucleotide resolution
Elsa D. M. Hien, Patrick St-Pierre, J. Carlos Penedo, Daniel A. Lafontaine Nucleic Acids Res, In revision 

    2023  
Multiple intermediates in the detergent induced fusion of lipid vesicles
L.G. Dresser, Casper Kunstmann, Donato Conteduca, STeven Johnson, J. Carlos Penedo, Mark C Leake, Steven D Quinn Communications materials, In revision 

    2023  
Conformational rearrangements regulating DNA repair protein APE1
Nina Komaniecka, Marta Porras, Louis Cairn, Jon Ander Santas, Juan Carlos Penedo, Sonia Bañuelos * Int. J. Mol. Sci. 23(14) 8015 

    2022  
Fatty acids may influence insulin actions through modulation of albumin-Zn2+ interactions
Swati Ayra, Adam Gourley, J. Carlos Penedo, Claudia Blindauer, Alan Stewart*. Bioessays. 2021;2100172.  

    2021  
Monitoring RNA dynamics within native transcriptional complexes 
Adrien Chauvier, Patrick St-Pierre, Jean-François Nadon, Elsa DM Hien, Cibran Perez-Gonzalez, Sébastien H. Eschbach, Anne-Marie Lamontagne, J. Carlos Penedo* and Daniel A. Lafontaine*. Proc.Natl. Acad. Sci. USA. Accepted, September 2021              

    2021  
A structural intermediate pre-organizes the add adenine riboswitch for ligand recognition
P. St-Pierre, E. Shaw, S. Jacques,P. A. Dalgarno, C. Perez-Gonzalez, F. Picard-Jean, J. Carlos Penedo* and Daniel A. Lafontaine* Nucleic Acids Res. Published 8 May, doi:  10.1093/nar/gkab307

    2021  
The influence of various regions of the FOXP2 sequence on its structure and DNA binding function 
Monare Thulo, Megan A. Rabie, Naadira Pahad, Heather L Donald, Ashleigh A Blane, Cardon M  Perumal, J. Carlos Penedo and Sylvia Fanucchi*   Biosci. Rep. doi.org/10.1042/BSR20202128 .

    2020  
Functional 3D architecture in an intrinsically disordered E3 ligase domain facilitates ubiquitin transfer
Paul Murphy, Yingqi Xu, Sarah L. Rouse, Steve J. Matthews, J. Carlos Penedo, Ron T. Hay*. Nat. Communications, 11, Article number: 3807.

    2020  
Ubiquitin transfer by a RING E3 ligase occurs from a closed E2-Ub conformation
Emma Branigan, J. Carlos Penedo*, Ron T. Hay*. Nat. Communications, 11, Article number: 2846     

    2020  
Asymmetric base pair opening drives helicase unwinding dynamics
Francesco Colizzi*, Cibran Perez-Gonzalez, Remi Fritzen, Yaakov Levy, Malcolm F. White, J. Carlos Penedo*, Giovanni Bussi*. Proc. Natl. Acad. Sci. USA (October 18, 2019 https://doi.org/10.1073/pnas.1901086116)

    2019  
Single-molecule spectroscopy of polyfluorene chains reveals beta-phase content and phase reversibility in organic solvents
Alfonso Brenlla, Francisco Tenopala-Carmona, Alexander L. Kanibolotsky, Peter J. Skabara*, Ifor D. W. Samuel*, J. Carlos Penedo*. Matter (Cell Press), Published:October 09, 2019 DOI:https://doi.org/10.1016/j.matt.2019.07.020

    2019  
Unveiling the multi-step solubilization mechanism of sub-micron size vesicles by detergents
Paul A. Dalgarno, Jose Juan Colas, Gordon J Hedley, Lucas Pineiro, Mercedes Novo, Cibran Perez-Gonzalez, Ifor D. W. Samuel, Mark C. Leake, Steven Johnson, Wajih Al-Soufi, J. Carlos Penedo* and Steven D. Quinn* Scientific Reports, 9:12897

    2019  

Unprecedented tunability of riboswitch structure and regulatory function by sub-millimolar variations in physiological Mg2+
Kaley McCluskey*, Julien Boudreault, Patrick St-Pierre, Cibran Perez-Gonzalez, Adrien Chauvier, Adrien Rizzi, Pascale B. Beauregard, Daniel A. Lafontaine* and J. Carlos Penedo*. Nucleic Acids Res. 47, 6478-6487

    2019  
Real-time observation of conformational switching in single conjugated polymers chains.
F. Tenopala-Carmona, S. Fronk, G. Bazan*, I. D. W. Samuel* and J. Carlos Penedo* Science Advances, Vol. 4, no. 2, eaao5786

    2018  
DNA binding and unwinding of forked DNA by Hel308 requires dual functions of a winged helix domain. Sarah J Northall, Ryan Buckey, Nathan Jones, J. Carlos Penedo, Panos Soultanas and Edward Bolt. DNA repair, 57, 125-132
    2017  
Transcriptional dynamics
Daniel A Lafontaine*, J. Carlos Penedo*. Editorial preface special topic in 'Transcriptional dynamics' Methods, Vol 120, 1-2
    2017  

An integrated perspective on RNA aptamer ligand recognition models: clearing muddy waters
Kaley McCluskey*, J. Carlos Penedo*. Phys. Chem. Chem. Phys. In press doi: 10.1039/C6CP08798A

    2017  
High affinity RNA binding by a hyperthermophilic single-stranded DNA binding protein
Michael Morten, Roland Gamsjaeger, Ruvini Kariawasam, Liza Cubeddu, Jose Peregrina, Elena Evgureieva-Hackenberg, J. Carlos Penedo and Malcolm F. White. Extremophiles. doi:10.1007/s00792-016-0910-2
    2017
 
Fluorescence-based strategies to investigate the structure and dynamics of RNA aptamer-ligand complexes
Cibran Perez-Gonzalez, Daniel A. Lafontaine*, J. Carlos Penedo* Frontiers in Chemistry, special topic in Natural and artificially-engineered nucleic acid aptamers: design, characterization and technological applications: Eds. Daniel A. Lafontaine and J. Carlos Penedo, Front. Chem. doi: 10.3389/fchem.2016.00033
    2016  
 
Morphology-specific inhibition of b-amyloid aggregates by 17b-hydroxysteroid dehydrogenase type 10
Laura Aitken, Steven D. Quinn, Daniel C. Perez-Gonzalez, Ifor D. W. Samuel, J. Carlos Penedo* and Frank Gunn-Moore* ChemBioChem. 17, 1029-1037.
    2016
 
Biophysical approaches to bacterial gene regulation by riboswitches
Cibran Perez-Gonzalez, Daniel A. Lafontaine* and J. Carlos Penedo* Advances in Experimental Medicine and Biology, 915, 157-191
    2016
 
Mechanism of DNA loading by the DNA repair helicase XPD
Diana Constantinescu Aruxandei, Biljana Petrovic-Stojanovska, J. Carlos Penedo, Malcolm F. White and James Naismith Nucl. Acids Res. (2016) doi: 10.1093/nar/gkw102
    2016
 
Binding dynamics of a monomeric SSB protein to DNA: a single-molecule multi-process approach
Michael J. Morten, Jose R. Peregrina, Maria Figueira-Gonzalez, Katrin Ackermann, Bela E Bode, Malcolm F. White* and J. Carlos Penedo* Nucleic Acids Res. 43(22), 10907-10924
    2015
 
Functional Studies of DNA-Protein Interactions Using FRET Techniques
S Blouin, TD Craggs, DA Lafontaine, JC Penedo DNA-Protein Interactions, Methods in Molecular Biology, 115-141
2015
 
Single-molecule approaches for the characterization of riboswitch folding mechanisms
Julien Boudreault, D. Cibran Perez-Gonzalez, J. Carlos Penedo and Daniel A. Lafontaine. DNA-Protein interactions: Methods and Protocols. Methods Mol. Biol. Vol 1334, 115-141
2015
 
Single-molecule strategies for DNA and RNA diagnostics
D. Cibran Perez-Gonzalez, J. Carlos Penedo, RNA Technologies:
RNA and DNA diagnostics. Springer-Verlag. Eds (D. A. Erdmann, S. Jurga and J. Barciszweski) pp 297-332
2015
 
Towards ratiometric sensing of amyloid fibrils in vitro
Sonia Freire, Flor Rodriguez-Prieto, M. Carmen Rios Rodriguez, J. Carlos Penedo, Wajih Al-Soufi and Mercedes Novo*,
Chemistry- A European Journal, 21, 3425 – 3434
2015
 
Using smFRET and denaturants to reveal folding landscapes
E. Shaw, K. McCluskey, P. St-Pierre, Daniel A. Lafontaine* and J. Carlos Penedo* Methods in Enzymology. Vol. 549, 313-341
2014
 
 
Phosphorylation of Hsp20 enhances its association with beta-amyloid to increase protection against neuronal cell death
Ryan T. Cameron, Steven D. Quinn, Lynn S. Z. Cairns, Ruth MacLeod, Brian O. Smith,Ifor D. W. Samuel, J. Carlos Penedo and George S. Baillie.Molecular and Cellular Neurosciences. In press .DOI: 1016/j.mcn.2014.05.002
2014
 
 
Fluorescence tools to investigate riboswitch structural dynamics
P. St-Pierre, K. McCluskey, E. Shaw, J. Carlos Penedo* and Daniel A Lafontaine*.BBA-Gene regulatory Mechanisms. In press. DOI: 10.1016/j.bbagrm.2014.05.015
2014
 
 
Single-molecule characterization of Fen1 and Fen1/PCNA complexes acting on flap substrates
Timothy D. Craggs, Richard D. Hutton, Alfonso Brenlla, Malcolm F. White and J. Carlos Penedo, Nucleic Acids Res. doi: 10.1093/nar/gkt1116
2014

 

 

Single-Molecule Fluorescence of Nucleic Acids
K McCluskey, E Shaw, DA Lafontaine, JC Penedo
Fluorescence Spectroscopy and Microscopy, Methods in Molecular Biology,1076 759-791
2013
Real-time probing of amyloid aggreation and inhibition using fluorescence self-quenching between neighbouring dyes
SD Quinn, PA Dalgarno, RT Cameron, GJ Hedley, C Hacker, JM Lucocq, GS Baillie, IDW Samuel, JC Penedo.Mol. BioSyst., 2014, 10 (1), 34 - 44

  2013
 
Solution-Based Single Molecule Imaging of Surface-Immobilized Conjugated Polymers
PA Dalgarno, CA Traina, JC Penedo, GC Bazan, IDW Samuel
Journal of the American Chemical Society
2013
 
 
Single-molecule chemical denaturation of riboswitches
PA Dalgarno, J Bordello, R Morris, P St-Pierre, A Dubé, IDW Samuel, DA, Daniel A. Lafontaine and J. Carlos Penedo Nucleic acids research
2013
 
Folding of the SAM-I riboswitch: A tale with a twist
SH Eschbach, P St-Pierre, JC Penedo, DA Lafontaine
RNA Biology 9 (5), 535-541
2012
Constitutive regulatory activity of an evolutionarily excluded riboswitch variant
R Tremblay, JF Lemay, S Blouin, J Mulhbacher, É Bonneau, P Legault, P Dupont ...
Journal of Biological Chemistry 286 (31), 27406-27415
2011
 
   
Molecular insights into the ligand-controlled organization of the SAM-I riboswitch
B Heppell, S Blouin, AM Dussault, J Mulhbacher, E Ennifar, JC Penedo, DA ...
Nature Chemical Biology 7 (6), 384-392
  2011
 
 
PCNA and XPF cooperate to distort DNA substrates
RD Hutton, TD Craggs, MF White, JC Penedo
Nucleic acids research 38 (5), 1664-1675
2010
 
Riboswitches: ancient and promising genetic regulators
S Blouin, J Mulhbacher, JC Penedo, DA Lafontaine
Chembiochem 10 (3), 400-416
2009
 
Natural Functional Nucleic Acids: Ribozymes and Riboswitches
R Tremblay, J Mulhbacher, S Blouin, JC Penedo, DA Lafontaine
Functional Nucleic Acids for Analytical Applications, 11-46
2009
 
Functional Studies of DNA-Protein Interactions Using FRET Techniques
S Blouin, TD Craggs, DA Lafontaine, JC Penedo
DNA-Protein Interactions, Methods in Molecular Biology, 475-502
2009
 
Molecular basis of RNA-mediated gene regulation on the adenine riboswitch by single-molecule approaches
JF Lemay, JC Penedo, J Mulhbacher, DA Lafontaine
Riboswitches, Methods in Molecular Biology, 65-76
2009
 
Application of fluorescent measurements for characterization of riboswitch–ligand interactions
B Heppell, J Mulhbacher, JC Penedo, DA Lafontaine
Riboswitches, Methods in Molecular Biology,25-37
2009
PCNA stimulates catalysis by structure-specific nucleases using two distinct mechanisms: substrate targeting and catalytic step
RD Hutton, JA Roberts, JC Penedo, MF White
Nucleic acids research 36 (21), 6720-6727
2008
Solvent dependent photophysics of fac-[Re (CO) 3 (11, 12-X2dppz)(py)]+(X= H, F or Me)
J Dyer, CM Creely, JC Penedo, DC Grills, S Hudson, P Matousek, AW Parker, M
Photochemical & Photobiological Sciences 6 (7), 741-748
2007
Folding of the adenine riboswitch
JF Lemay, JC Penedo, R Tremblay, DMJ Lilley, DA Lafontaine
Chemistry & Biology 13 (8), 857-868
2006
 
Two competitive routes in the lactim-lactam phototautomerization of a hydroxypyridine derivative cation in water: dissociative mechanism versus water-assisted proton transfer
JC Penedo, MC Ríos Rodríguez, I García Lema, JL Pérez Lustres, M Mosquera, F ...
The Journal of Physical Chemistry A 109 (45), 10189-10198
2005
 
Photophysical study of a family of [Ru (phen) 2 (Mendpq)] 2+ complexes in different solvents and DNA: a specific water effect promoted by methyl substitution
K O'Donoghue, JC Penedo, JM Kelly, PE Kruger
Dalton Transactions, 1123-1128
2005
Solvent-dependent ground-and excited-state tautomerism in 2-(6'-hydroxy-2'-pyridyl) benzimidazole
JC Penedo, JLP Lustres, IG Lema, MCR Rodríguez, M Mosquera, F Rodríguez-Prieto
The Journal of Physical Chemistry A 108 (29), 6117-6126
2004
 
Folding of the natural hammerhead ribozyme is enhanced by interaction of auxiliary elements
JC PENEDO, TJ WILSON, SD JAYASENA, A KHVOROVA, DMJ LILLEY
RNA 10 (5), 880-888
2004
Ultrafast transient absorption studies of ruthenium and rhenium dipyridophenazine complexes bound to DNA and polynucleotides
CM Creely, JM Kelly, MM Feeney, S Hudson, JC Penedo, WJ Blau, B Elias, A ...
OPTO Ireland, Proceedings SPIE, 92-102
2003
Role of hydrogen-bonded adducts in excited-state proton-transfer processes
JC Penedo, M Mosquera, F Rodríguez-Prieto
The Journal of Physical Chemistry A 104 (32), 7429-7441
2000
On the Mechanism of Alcohol-Catalyzed Excited-State Intramolecular Proton Transfer in Cationic Benzimidazoles
MCR Rodríguez, JC Penedo, RJ Willemse, M Mosquera, F Rodríguez-Prieto
The Journal of Physical Chemistry A 103 (36), 7236-7243
1999
Solvent control of molecular structure and excited-state proton-transfer processes of 1-methyl-2-(2′-hydroxyphenyl)-benzimidazole
F Rodríguez-Prieto, JC Penedo, M Mosquera
Journal of the Chemical Society, Faraday Transactions 94 (18), 2775-2782
1998
Photoinduced Inter-and Intramolecular Proton Transfer in Aqueous and Ethanolic Solutions of 2-(2'-Hydroxyphenyl) benzimidazole: Evidence for Tautomeric and Conformational Equilibria in the Ground State
M Mosquera, JC Penedo, MC Ríos Rodríguez, F Rodríguez-Prieto
The Journal of Physical Chemistry 100 (13), 5398-5407
1996