2A in Biomedicine & Biotechnology

Research papers using 2A, plus reviews discussing the use of 2A

Comment upon Sommer et al., (2009). iPS cell generation using a single lentiviral stem cell cassette. Stem Cells 27, 543-549.

'Converting Adult Somatic Cells to Pluripotent Stem Cells using a Single Virus' - Physorg.com

Comment upon Holst et al., (2006). Rapid analysis of T-cell selection in vivo using T cell-receptor retrogenic mice. Nature Methods 3, 191-197.

'Retroviral TCR Gene Transduction: 2A for Two'. Bosselut, R. (2006). News & Views, Nature Methods 3, 162-164.

Comment upon Fang et al., (2005). Stable antibody expression at therapeutic levels using the 2A peptide. Nature Biotechnol. 23, 584-590.

'Therapeutic Antibody Gene Transfer' - 'News and Views' discussion the technology for gene therapy in Nature Biotechnology.

'Viral 2A Peptide Stabilizes Monoclonal Antibody at Therapeutic Levels' Gene Therapy Weekly

Comment upon Szymczak et al., (2004). Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector. Nature Biotechnol. 22, 589-94.

'Put all your Genes in One Basket ' - a highlight of the technology for gene therapy in Nature Methods

'Caught in Translation ' - a highlight of the technology for gene therapy in Nature Immunology

'Multiple Gene products from a single vector; 'self-cleaving 2A peptides' Gene Therapy 11, 1673-1674 (2004).

'Virus Trick Could Turn Cells into Drug Factories' Better Humans

'Genetic Trick Adapted From Viruses Makes Gene Therapy Vectors More Versatile'

Science Daily ..Innovations Report...Charity Wire...St. Judes Childrens Research Hospital.. News-Medical.Net.... Techmonitor.net..bio.com

'Gene Therapy Enhanced by Virus Peptides' .....MedMarkets


A new function (translational recoding plus co-translational signal sequence) of cellular "2A-like" sequences that produces dual protein targeting...

Roulston, C., Luke, G.A., de Felipe, P., Ruan, L., Cope, J., Nicholson, J., Sukhodub, A., Tilsner, J. & Ryan, M.D. (2016). ‘2A-Like’ signal sequences mediating translational recoding: A novel form of dual protein targeting. Traffic 17, 923-939.


1467. Bahar, M.W., Porta, C., Fox, H., Macadam, A.J., Fry, E.E. & Stuart, D.I. (2021). Mammalian expression of virus-like particles as a proof of principle for next generation polio vaccines. NPJ Vaccines 6(1):5.

1466. Cha, S.E., Kujawski, M.J., Yazaki, P., Brown, C. & Shively, J.E. (2021). Tumor regression and immunity in combination therapy with anti-CEA chimeric antigen receptor T cells and anti-CEA-IL2 immunocytokine. Oncoimmunology 10(1):1899469.

1465. Choi, E.H., Suh, S., Einstein, D.E, Leinonen, H., Dong, Z., Rao, S.R., Fliesler, S.J., Blackshaw, S., Yu, M., Peachey, N.S, Palczewski, K. & Kiser, P.D. (2021). An inducible Cre mouse for studying roles of the RPE in retinal physiology and disease. JCI Insight Mar 30;146604.

1464. Chowdhury, E.A., Meno-Tetang, G., Chang, H.Y., Wu, S., Huang, H.W., Jamier, T., Chandran, J. & Shah, D.K. (2021). Current progress and limitations of AAV mediated delivery of protein therapeutic genes and the importance of developing quantitative pharmacokinetic/pharmacodynamic (PK/PD) models. Adv. Drug Deliv. Rev. 170, 214-237.

1463. Dahlin, L.R & Guarneri, M.T. (2021). Development of the high-productivity marine microalga, Picochlorum renovo, as a photosynthetic protein secretion platform. Algal Res. 54102197.

1462. Efimova, V.S., Isaeva, L.V., Orekhov, P.S., Bozdaganyan, M.E., Rubtsov, M.A. & Novikova, L.A. (2021). Using a viral 2A peptide-based strategy to reconstruct the bovine P450scc steroidogenic system in S. cerevisiae Bovine P450scc system expression using 2A peptides. J. Biotechnol. 325, 186-195.

1461. Gallaher, S.D., Craig, R.J., Ganesan, I., Purvine, S.O., McCorkle, S.R., Grimwood, J., Strenkert, D., Davidi, L., Roth, M.S., Jeffers, T.L., Lipton, M.S., Niyogi, K.K., Schmutz, J. Theg, S.M., Blaby-Haas, C.E. & Merchant, S.S. (2021). Widespread polycistronic gene expression in green algae. Proc. Natl. Acad. Sci. USA. 118(7):e2017714118.

1460. Goldman, D.H., Livingston, N.M., Movsik, J., Wu, B. & Green, R. (2021). Live-cell imaging reveals kinetic determinants of quality control triggered by ribosome stalling. Mol. Cell 81, 1–11.

1459. González-Romero, M.E., Rivera, C., Cancino, K., Geu-Flores, F., Cosio, E.G., Ghislain, M. & Halkier. B.A. (2021). Bioengineering potato plants to produce benzylglucosinolate
for improved broad-spectrum pest and disease resistance. Transgenic Res. doi: 10.1007/s11248-021-00255-w. Online ahead of print.

1458. Hudson, A.M., Szabo, N.L., Loughran, G., Wills, N.M., Atkins, J.F. & Cooley, L. (2021). Tissue-specific dynamic codon redefinition in Drosophila. Proc. Natl. Acad. Sci. USA. 118(5):e2012793118..

1457. Jang, H., Park, Y.H., Kim, M-H., You, J., Ko, J-H, & Lee, J.T. (2021). Surface characteristics of porous carbon derived from genetically designed transgenic hybrid poplar for electric double-layer capacitors. App. Surface Sci. 545:148978.

1456. Jeong, Y,S., Ku, H-K., Jung, Y-J., Kim, J.K., Lee, K.B., Kim, J-K., Lim, S-H., Lee, D. & Ha, S.H. (2021). 2A-linked bi-, tri-, and quad-cistrons for the stepwise biosynthesis of ß-carotene, zeaxanthin, and ketocarotenoids in rice endosperm. Metab. Eng. Commun. 12:e00166.

1455. Lo, Y-T., Tulloch, F., Wu, H.C., Luke, G.A., Ryan, M.D. & Chu, C-Y. (2021). Expression and immunogenicity of secreted forms of bovine ephemeral fever virus glycoproteins applied to subunit vaccine development. J. Appl. Microbiol. Feb 18. doi: 10.1111/jam.15044. Online ahead of print.

1454. Meas, S., Mekvichitsaeng, P. & Roshorm, Y.M. (2021). Co-expression of self-cleaved multiple proteins derived from Porcine Reproductive and Respiratory Syndrome Virus by bi-cistronic and tri-cistronic DNA vaccines. Protein Expr. Purif. 177:105763.

1453. Müller, J.P., Scholl, S., Kunick, C. & Klempnauer, K.H. (2021). Expression of protein kinase HIPK2 is subject to a quality control mechanism that acts during translation and requires its kinase activity to prevent degradation of nascent HIPK2. Biochim. Biophys. Acta Mol. Cel.l Res. 1868(1):118894.

1452. Rand, U., Kubsch, T., Kasmapour, B. and Cicin-Sain, L. (2021). A novel triple-fluorescent HCMV strain reveals gene expression dynamics and anti-herpesviral drug mechanisms. Front. Cell Infect. Microbiol. 10, 536150.

1451. Rozen-Gagnon, K., Yi, S., Jacobson, E., Novack, S. & Rice, C.M. (2021). A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines. Sci Rep. 11(1):736.

1450. Sádlová, J., Podešvová, L., Becvár, T., Bianchi, C., Gerasimov, E.S., Saura, A., Glanzová, K., Leštinová, T., Matveeva, N.S., Chmelová, L., Mlacovská, D., Spitzová, T., Vojtková, B., Volf, P., Yurchenko, V. & Kraeva, N. (2021). Catalase impairs Leishmania mexicana development and virulence. Virulence 12, 852-867.

1449. Scarborough. A.M., Flaherty, J.N., Hunter, O.V., Liu, K., Kumar, A., Xing, C., Tu, B.P. & Conrad, N.K. (2021). SAM homeostasis is regulated by CFI(m)-mediated splicing of MAT2A. eLife 10:e64930.

1448. Shibata, A.C.E., Ueda, H.H., Eto, K., Onda, M., Sato, A., Ohba, T., Nabekura, J. & Murakoshi, H. (2021). Photoactivatable CaMKII induces synaptic plasticity in single synapses. Nat. Commun. 12(1):751.

1447. Shao, Q., Jia, X., Gao, Y., Liu, Z., Zhang, H., Tan, Q., Zhang, X., Zhou, H., Li, Y., Wu, D. & Zhang, Q. (2021). Cryo-EM reveals a previously unrecognized structural protein of a dsRNA virus implicated in its extracellular transmission. PLoS Pathog. 17(3):e1009396.

1446. Tasker, C., Patel, J., Jawa, V. & Maamary, J. (2021). Competition-based cell assay employing soluble T cell receptors to assess MHC class II antigen processing and presentation. AAPS J. 23(2):26.

1445. Wang, M., Mou, C., Chen, M. & Chen, Z. (2021). Infectious recombinant Senecavirus A expressing novel reporter proteins. Appl. Microbiol. Biotechnol. 105, 2385-2397.

1444. Work, J.J & Brandman, O. (2021). Adaptability of the ubiquitin-proteasome system to proteolytic and folding stressors. J. Cell. Biol. 220:e201912041.

1443. Xiong, Z., Lo, H.P., McMahon, K.A., Martel, N., Jones, A., Hill, M.M., Parton, R.G. & Hall, T.E. (2021). In vivo proteomic mapping through GFP-directed proximity-dependent biotin labelling in zebrafish. eLife 10:e64631.

1442. Yasuda, T., Saito,Y., Ono, C., Kawata, K., Baba, A. & Baba, Y. (2021). Generation and characterization of CD19-iCre mice as a tool for efficient and specific conditional gene targeting in B cells. Sci. Rep. 11:5524

1441. Zhang, H., Zheng, Q. & Chen-Tsai, R.Y. (2021). Establishment of a Cre-rat resource for creating conditional and physiological relevant models of human diseases. Transgenic Res. 30, 91-104.


1440. Alarcón, H., Bonzon-Kulichenko, E., Peinado, R., Lim, F., Vázquez, J. & Rodríguez, A. (2020). Generation of a lentiviral vector system to efficiently express bioactive recombinant human prolactin hormones. Mol. Cell. Endocrinol. 499, 110605.

1439. Ando, R., Sakaue-Sawano, A., Shoda, K., Miyawaki, A., 2020. Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv, 6.
https://doi.org/10.1101/2020.03.30.015156, 2020.03.30.01515.

1438. Arevalo-Villalobos, J., Govea-Alonso, D., Bañuelos-Hernández, B., González-Ortega, O., Zarazúa, S. & Rosales-Mendoza, S. (2020). Inducible expression of antigens in plants: a study focused on peptides related to multiple sclerosis immunotherapy. J. Biotechnol. 318, 51-56.

1437. Barakate, A., Keir, E., Oakey, H. & Halpin, C. (2020). Stimulation of homologous recombination in plants expressing heterologous recombinases. BMC Plant Biology 20, 336.

1436. Bohlender, L.L., Parsons, J., Hoernstein, S.N.W., Rempfer, C., Ruiz-Molina, N., Lorenz, T., Rodríguez Jahnke, F., Figl, R., Fode, B., Altmann, F., Reski, R. & Decker, E.L. (2020). Stable Protein Sialylation in Physcomitrella. Front. Plant Sci. 11:610032.

1435. Cai, L., Hu, H., Duan, H., Li, Y., Zou, Z., Luo, K., Zhang, Z., Yang, J., Jin, J., Chen, Y., Ke, Z., Fang, Z., Liu, Q., Hong, X., Hu, S. & Liu, B. (2020). The construction of a new oncolytic herpes simplex virus expressing murine interleukin-15 with gene-editing technology. J. Med. Virol. doi: 10.1002/jmv.25691. Online ahead of print.

1434. Chiale, C., Yarovinsky, T.O., Mason, S.W., Madina, B.R., Menon, M., Krady, M.M., Moshkani, S., Chattopadhyay Pal, A., Almassian, B., Rose, J.K., Robek, M.D. & Nakaar, V. (2020). Modified alphavirus-vesiculovirus hybrid vaccine vectors for homologous prime-boost immunotherapy of chronic hepatitis B. Vaccines (Basel) 8(2):279.

1433. Chu, I-R., Pan, R-L., Yang, C-S. and Wu, L-C. (2020). A doxycycline-inducible C17.2 neural stem cell-based combination of differentiation and suicide gene therapy for an in vitro tumorigenic C6 glioma model. Biotech. Biotechnological Equipment 34, 986-998.

1432. Costello, A., Lao, N.T., Barron, N. & Clynes, M. (2020). Reinventing the wheel: synthetic circular RNAs for mammalian cell engineering. Trends Biotechnol. 38, 217-230.

1431. Crowther, M, D., Dolton, G., Legut, M., Caillaud, M, E., Lloyd, A., Attaf, M., Galloway, S., Rius, C., Farrell, C, P., Szomolay, B., Ager, A., Parker, A, L., Fuller, A., Donia, M., McCluskey, J., Rossjohn, J., Svane, I, M., Phillips, J, D. & Sewell, A, K. (2020). Genome-wide CRISPR-Cas9 screening reveals ubiquitous T cell cancer targeting via the monomorphic MHC class I-related protein MR1. Nat. Immunol. 21,178-185.

1430. Cruz-Teran, C., Bacon, K. & Rao, BM. Eds: Zielonka, S. & Krah, S. (2020). Simultaneous soluble secretion and surface display of proteins in saccharomyces cerevisiae using inefficient pibosomal Sskipping. Genotype Phenotype Coupling: Methods And Protocols 2070, 321-334.

1429. Davola, M, E., Vito, A., Wei, J., El-Sayes, N., Workenhe, S. & Mossman, K, L. Eds: Galluzzi, L. & Rudqvist, N, P. (2020). Genetic modification of oncolytic viruses to enhance antitumor immunity. Tumor Immunology And Immunotherapy - Integrated Methods 635, 231-250.

1428. Dehghani, J., Adibkia, K., Movafeghi, A., Pourseif, M, M. & Omidi, Y. (2020). Designing a new generation of expression toolkits for engineering of green microalgae; robust production of human interleukin-2. Bioimpacts 10, 257-266.

1427. Del Papa, J., Clarkin, R, G. & Parks, R, J. (2020). Use of cell fusion proteins to enhance adenoviral vector efficacy as an anti-cancer therapeutic. Cancer Gene Therapy 2020 Jul 1. doi: 10.1038/s41417-020-0192-9

1426. Dhamija, S., Yang, C.M., Seiler, J., Myacheva, K., Caudron-Herger, M., Wieland, A., Abdelkarim, M., Sharma, Y., Riester, M., Groß, M., Maurer, J. & Diederichs, S. (2020). A pan-cancer analysis reveals nonstop extension mutations causing SMAD4 tumour suppressor degradation. Nat. Cell Biol. 22, 999-1010.

1425. Dorrington, R.A, Jiwaji, M., Awando, J.A & de Bruyn, M-M. (2020). Advances in Tetravirus research: new insight into the infectious virus life Cycle and an expanding host range. Curr. Issues Mol. Biol. 34, 145-162.

1424. El Fekih, S., Tous, C., Gueganic, N., Brugnon, F., Ben Ali, H., Bujan, L., Moinard, N., Caire-Tetauru, E., Ajina, M., Douet-Guilbert, N., Morel, F. & Perrin, A. (2020). Decrease of spermatozoa with an unbalanced chromosome content after cell sorting in men carrying a structural chromosomal abnormality. Andrology 8(1), 181-190.

1423. Farnworth, M, S., Eckermann, K, N., Ahmed, K, M., Mühlen, D, S., He, B. & Bucher, G. (2020). The red flour beetle as model for comparative neural development: Genome editing to mark neural cells in tribolium brain development. Methods Mol Biol. 2047, 191-217.

1422. Farrera-Sal, M., de Sostoa, J., Nuñez-Manchón, E., Moreno, R., Fillat, C., Bazan-Peregrino, M. & Alemany, R. (2020). Arming oncolytic adenoviruses: Effect of insertion site and splice acceptor on transgene expression and viral fitness. Int. J. Mol. Sci. 21(14):5158.

1421. Farrera-Sal, M., Fillat, C. & Alemany, R. (2020). Effect of transgene location, transcriptional control elements and transgene features in armed oncolytic adenoviruses. Cancers (Basel). 12(4):1034.

1420. Fuller, J., Álvarez-Rodríguez, B., Todd, E., Mankouri, J., Hewson, R. & Barr, J, N. (2020). Hazara Nairovirus Requires COPI Components in both Arf1-Dependent and Arf1-Independent Stages of Its Replication Cycle. J. Virol. 94, e00766-20.

1419. Garcia-Gonzalez, I., Mühleder, S., Fernández-Chacón, M. & Benedito, R. (2020). Genetic tools to study cardiovascular biology. Front Physiol. 11:1084.

1418. Gioseffi, A., Hamerly, T., Van, K., Zhang, N., Dinglasan, R.R., Yates, P.A. & Kima, P.E. (2020). Leishmania-infected macrophages release extracellular vesicles that can promote lesion development. Life Sci Alliance 3(12):e202000742..

1417. Guseva, S., Milles, S., Jensen, M, R., Kleman, J., Maurin, D., Ruigrok, R. & Blackledge, M. (2020). Measles virus nucleo- and phosphoproteins form liquid-like phase-separated compartments that promote nucleocapsid assembly. Sci Adv. 6, eaaz7095.

1416. Hao, J., Yang, X., Zhang, C., Zhang, X.T., Shi, M., Wang, S.H., Mi, L., Zhao, Y.T., Cao, H. & Wang, Y. (2020). KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells. Cell Prolif. 53(11):e12914.

1415. Han, P., Shichino, Y., Schneider-Poetsch, T., Mito, M., Hashimoto, S., Udagawa, T., Kohno, K., Yoshida, M., Mishima, Y., Inada, T. & Iwasaki, S. (2020). Genome-wide Survey of Ribosome Collision. Cell Reports. 31, (5).

1414. Hannemann, H. (2020). Viral replicons as valuable tools for drug discovery. Drug Discov. Today. 25, 1026-1033.

1413. He, Y., Zhang, T., Sun, H., Zhan, H. & Zhao, Y. (2020). A reporter for noninvasively monitoring gene expression and plant transformation. Hortic. Res. 7:152.

1412. Hinz, S, C., Elter, A., Grzeschik, J., Habermann, J., Becker, B. & Kolmar, H. (2020). Simplifying the detection of surface presentation levels in yeast surface display by intracellular tgfp expression. Methods Mol. Biol. 211-222.

1411. Huang, J., Wang, A., Huang, C., Sun, Y., Song, B., Zhou, R. & Li, L. (2020). Generation of marker-free pbd-2 knock-in pigs using the CRISPR/Cas9 and Cre/loxP systems. Genes (Basel) 11(8):951.

1410. Ibrahim, S., Harris-Kawano, A., Haider, I., Mirmira, R, G., Sims, E, K. & Anderson, R, M. (2020). A novel Cre-enabled tetracycline-inducible transgenic system for tissue-specific cytokine expression in the zebrafish: CETI-PIC3. Dis. Model Mech. 13(6).

1409. Jaffri, S.A., Yan, Y., Schwirz, J. & Schetelig, M.F. (2020). Functional characterization of the Drosophila suzukii pro-apoptotic genes reaper, head involution defective and grim. Apoptosis 25, 864-874.

1408. Ji, Y., Li, B., Qiao, M., Li, J., Xu, H., Zhang, L. & Zhang, X. (2020). Advances on the in vivo and in vitro glycosylations of flavonoids. Appl. Microbiol. Biotechnol. 104, 6587-6600.

1407. Juszkiewicz, S., Slodkowicz, G., Lin, Z., Freire-Pritchett, P., Peak-Chew, S.Y. & Hegde, R.S. (2020). Ribosome collisions trigger cis-acting feedback inhibition of translation initiation. eLife 9:e60038.

1406. Khosla, A. & Nelson, D.C. (2020). Ratiometric measurement of protein abundance after transient expression of a transgene in Nicotiana benthamiana. BioProtoc. 10(17):e3747.

1405. Kelly, F.D., Tran, K.D., Hatfield, J., Schmidt, K., Sanchez, M.A. and Landfear, S.M. (2020). A cytoskeletal protein complex is essential for division of intracellular amastigotes of Leishmania mexicana. J. Bio.l Chem. 295, 13106-13122.

1404. Khosla, A., Rodriguez-Furlan, C., Kapoor, S, J., Van Norman, J. & Nelson, D, C. (2020). A series of dual-reporter vectors for ratiometric analysis of protein abundance in plants. Plant Direct. 4, e00231.

1403. Khosla, A., Morffy, N., Li, Q., Faure, L., Chang, S.H., Yao, J., Zheng, J., Cai, M.L., Stanga, J., Flematti, G.R., Waters, M.T. & Nelson, D.C. (2020). Structure-Function Analysis of SMAX1 reveals domains that mediate its Karrikin-induced proteolysis and interaction with the receptor KAI2. Plant Cell 32, 2639-2659.

1402. Kojin, B, B., Biedler, J, K., Tu, Z. & Adelman, Z, N. (2020). Characterization of a female germline and early zygote promoter from the transcription factor bZip1 in the dengue mosquito Aedes aegypti. Parasit. Vectors 13, 353.

1401. Komoto, S., Fukuda, S., Hatazawa, R., Murata, T. & Taniguchi, K. (2020). Generation of recombinant rotaviruses from just 11 cDNAs encoding a viral genome. Virus Res. 286:198075.

1400. Kondo, S., Takahashi, T., Yamagata, N., Imanishi, Y., Katow, H., Hiramatsu, S., Lynn, K., Abe, A., Kumaraswamy, A. & Tanimoto, H. (2020). Neurochemical organization of the drosophila brain visualized by endogenously tagged neurotransmitter receptors. Cell Rep. 30, 284-297.

1399. Krah, S., Günther, S., Becker, S., Zielonka, S. & Rhiel, L. (2020). Chemical modification of the yeast cell surface allows the switch between display and soluble secretion of full-length antibodies. Methods in Microbiology 2070, 335-349.

1398. Krishnamurthy, V, V., Fu, J., Oh, T., Khamo, J., Yang, J. & Zhang, K. (2020). A generalizable optogenetic strategy to regulate receptor tyrosine kinases during vertebrate embryonic development. J. Mol. Biol. 432, 3149-3158.

1397. Kropp, K.A., Srivaratharajan, S., Ritter, B., Yu, P., Krooss, S., Polten, F., Pich, A., Alcami, A. & Viejo-Borbolla, A. (2020). Identification of the cleavage domain within glycoprotein G of Herpes Simplex Virus Type 2. Viruses 12(12):1428.

1396. Lee, J.H., Won, H.J., Oh, E.S., Oh, M.H. & Jung, J.H. (2020). Golden gate cloning-compatible DNA replicon/2A-mediated polycistronic vectors for plants. Front. Plant Sci. 11:559365.

1395. Levin, S, D., Evans, L, S., Bort, S., Rickel, E., Lewis, K, E., Wu, R, P., Hoover, J., MacNeil, S., La, D., Wolfson, D, F., Rixon, M, W., Dillon, S, R., Kornacker, M, G., Swanson, R. & Peng, S, L. (2020). Novel immunomodulatory proteins generated via directed evolution of variant IgSF domains. Front. Immunol. 10, 3086.

1394. Li, H., Zhang, Q., Gu, Y., Wu, Y., Wang, Y., Wang, L., Feng, S., Hu, Y., Zheng, Y., Li, Y., Ye, H., Zhou, B., Lin, L., Liu, M., Yang, H. & Li, D. (2020). Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse. Proc. Natl. Acad. Sci. USA. 117, 33426-33435.

1393. Li, X., Bi, Z., Sun, Y., Li, C., Li, Y. & Liu, Z. (2020). In vivo ectopic Ngn1 and Neurod1 convert neonatal cochlear glial cells into spiral ganglion neurons. FASEB J. 34, 4764-4782.

1392. Li, Y.M., Wang, M., Wang, T.Y., Wei, Y.G., Guo, X., Mi, C.L., Zhao, C.P., Cao, X.X. & Dou, Y.Y. (2020). Effects of different 2A peptides on transgene expression mediated by tricistronic vectors in transfected CHO cells. Mol. Biol. Rep. 47, 469-475.

1391. Licon, M, H. & Yates, P, A. (2020). Purine-responsive expression of the Leishmania donovani NT3 purine nucleobase transporter is mediated by a conserved RNA stem-loop. J. Biochem. 25, 8449-8459.

1390. Lin, Y., Weng, K., Liang, J., Zhou, Y., Hao, Q., Hao, Y., Yao, K. & Zou, J. (2020). The extracellular and intracellular regions of Crb2a play distinct roles in guiding the formation of the apical zonula adherens. Biomed. Pharmacother. 125, 109942.1382.

1389. Lin, Y.Y., Schuphan, J., Dickmeis, C., Buhl, E.M., Commandeur, U., Fischer, H. (2020). Attachment of ultralow amount of engineered plant viral nanoparticles to mesenchymal stem cells enhances osteogenesis and mineralization. Adv. Healthc. Mater. 9(21):e2001245..

1388. Matreyek, K.A., Stephany, J.J., Chiasson, M.A., Hasle, N. & Fowler, D.M. (2020). An improved platform for functional assessment of large protein libraries in mammalian cells. Nuc. Acids Res. 48(1):e1.

1387. Matsunaga, S., Jeremiah, S, S., Miyakawa, K., Kurotaki, D., Shizukuishi, S., Watashi, K., Nishitsuji, H., Kimura, H., Tamura, T., Yamamoto, N., Shimotohno, K., Wakita, T. & Ryo, A. (2020). Engineering cellular biosensors with customizable antiviral responses targeting Hepatitis B Virus. iScience 23:100867.

1386. Mizote, Y., Masumi-Koizumi, K., Katsuda, T. & Yamaji, H. (2020). Production of an antibody Fab fragment using 2A peptide in insect cells. J. Biosci. Bioeng. 130, 205-211.

1385. Mizushima, R., Inoue, K., Fujiwara, H., Iwane, H., Watanabe, T, M. & Kimura, A. (2020). Multiplexed 129 Xe hyperCEST MRI detection of genetically reconstituted bacterial protein nanoparticles in human cancer cells. Contrast Media & Molecular Imaging. 5425934.

1384. Molina-Márquez, A., Vila, M., Rengel, R., Fernández, E., García-Maroto, F., Vigara, J. & León, R. (2020). Validation of a new multicistronic plasmid for the efficient and stable expression of transgenes in microalgae. Int. J. Mol. Sci. 21, 718.

1383. Morgan, R, A., Ma, F., Unti, M, J., Brown, D., Ayoub, P, G., Tam, C., Lathrop, L., Aleshe, B., Kurita, R., Nakamura, Y., Senadheera, S., Wong, R, L., Hollis, R, P., Pellegrini, M. & Kohn, B, D. (2020). Creating new ß-globin-expressing lentiviral vectors by high-resolution mapping of locus control region enhancer sequence. Mol. Ther. Methods Clin. Dev. 18, 999-1013.

1382. Mu, C., Vakharia, V.N., Zhou, Y., Jiang, N., Liu, W., Meng, Y., Li, Y., Xue, M., Zhang, J., Zeng, L., Zhong, Q. and Fan, Y. (2020). A novel subunit vaccine based on outer capsid proteins of Grass Carp Reovirus (GCRV) provides protective immunity against GCRV infection in Rare Minnow (Gobiocypris rarus). Pathogens 9, 945.

1381. Muhammad, B. B., Yusoff, K., Ideris, A., Hair-Bejo, M., Jibril, A., Peeters, B. & Omar, A. (2020). Exploring the prospects of engineered Newcastle Disease Virus in modern vaccinology. Viruses 12(4):451.

1380. Müntjes, K., Philipp, M., Hüsemann, L., Heucken, N., Weidtkamp-Peters, S., Schipper, K., Zurbriggen, M, D. & Feldbrügge, M. (2020). Establishing polycistronic expression in the model microorganism Ustilago maydis. Front. Microbiol. 11,1384.

1379. Murakoshi, H. (2021). Optogenetic imaging of protein activity using two-photon fluorescence lifetime imaging microscopy. Adv. Exp. Med. Biol. 1293, 295-308.

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1. Ryan, M.D. & Drew, J. (1994). Foot-and-mouth disease virus 2A oligopeptide mediated cleavage of an artificial polyprotein. EMBO J. 134, 928-933.

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