2A in Biomedicine & Biotechnology

Research papers using 2A, plus reviews discussing the use of 2A


Comment upon Sommer et al., (2009). iPS cell generation using a single lentiviral stem cell cassette. Stem Cells 27, 543-549.

'Converting Adult Somatic Cells to Pluripotent Stem Cells using a Single Virus' - Physorg.com


Comment upon Holst et al., (2006). Rapid analysis of T-cell selection in vivo using T cell-receptor retrogenic mice. Nature Methods 3, 191-197.

'Retroviral TCR Gene Transduction: 2A for Two'. Bosselut, R. (2006). News & Views, Nature Methods 3, 162-164.


Comment upon Fang et al., (2005). Stable antibody expression at therapeutic levels using the 2A peptide. Nature Biotechnol. 23, 584-590.

'Therapeutic Antibody Gene Transfer' - 'News and Views' discussion the technology for gene therapy in Nature Biotechnology.

'Viral 2A Peptide Stabilizes Monoclonal Antibody at Therapeutic Levels' Gene Therapy Weekly


Comment upon Szymczak et al., (2004). Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector. Nature Biotechnol. 22, 589-94.

'Put all your Genes in One Basket ' - a highlight of the technology for gene therapy in Nature Methods

'Caught in Translation ' - a highlight of the technology for gene therapy in Nature Immunology

'Multiple Gene products from a single vector; 'self-cleaving 2A peptides' Gene Therapy 11, 1673-1674 (2004).

'Virus Trick Could Turn Cells into Drug Factories' Better Humans

'Genetic Trick Adapted From Viruses Makes Gene Therapy Vectors More Versatile'

Science Daily ..Innovations Report...Charity Wire...St. Judes Childrens Research Hospital.. News-Medical.Net.... Techmonitor.net..bio.com

'Gene Therapy Enhanced by Virus Peptides' .....MedMarkets

 

A new function (translational recoding plus co-translational signal sequence) of cellular "2A-like" sequences that produces dual protein targeting...

Roulston, C., Luke, G.A., de Felipe, P., Ruan, L., Cope, J., Nicholson, J., Sukhodub, A., Tilsner, J. & Ryan, M.D. (2016). ‘2A-Like’ signal sequences mediating translational recoding: A novel form of dual protein targeting. Traffic 17, 923-939.


2021

1467. Bahar, M.W., Porta, C., Fox, H., Macadam, A.J., Fry, E.E. & Stuart, D.I. (2021). Mammalian expression of virus-like particles as a proof of principle for next generation polio vaccines. NPJ Vaccines 6(1):5.

1466. Cha, S.E., Kujawski, M.J., Yazaki, P., Brown, C. & Shively, J.E. (2021). Tumor regression and immunity in combination therapy with anti-CEA chimeric antigen receptor T cells and anti-CEA-IL2 immunocytokine. Oncoimmunology 10(1):1899469.

1465. Choi, E.H., Suh, S., Einstein, D.E, Leinonen, H., Dong, Z., Rao, S.R., Fliesler, S.J., Blackshaw, S., Yu, M., Peachey, N.S, Palczewski, K. & Kiser, P.D. (2021). An inducible Cre mouse for studying roles of the RPE in retinal physiology and disease. JCI Insight Mar 30;146604.

1464. Chowdhury, E.A., Meno-Tetang, G., Chang, H.Y., Wu, S., Huang, H.W., Jamier, T., Chandran, J. & Shah, D.K. (2021). Current progress and limitations of AAV mediated delivery of protein therapeutic genes and the importance of developing quantitative pharmacokinetic/pharmacodynamic (PK/PD) models. Adv. Drug Deliv. Rev. 170, 214-237.

1463. Dahlin, L.R & Guarneri, M.T. (2021). Development of the high-productivity marine microalga, Picochlorum renovo, as a photosynthetic protein secretion platform. Algal Res. 54102197.

1462. Efimova, V.S., Isaeva, L.V., Orekhov, P.S., Bozdaganyan, M.E., Rubtsov, M.A. & Novikova, L.A. (2021). Using a viral 2A peptide-based strategy to reconstruct the bovine P450scc steroidogenic system in S. cerevisiae Bovine P450scc system expression using 2A peptides. J. Biotechnol. 325, 186-195.

1461. Gallaher, S.D., Craig, R.J., Ganesan, I., Purvine, S.O., McCorkle, S.R., Grimwood, J., Strenkert, D., Davidi, L., Roth, M.S., Jeffers, T.L., Lipton, M.S., Niyogi, K.K., Schmutz, J. Theg, S.M., Blaby-Haas, C.E. & Merchant, S.S. (2021). Widespread polycistronic gene expression in green algae. Proc. Natl. Acad. Sci. USA. 118(7):e2017714118.

1460. Goldman, D.H., Livingston, N.M., Movsik, J., Wu, B. & Green, R. (2021). Live-cell imaging reveals kinetic determinants of quality control triggered by ribosome stalling. Mol. Cell 81, 1–11.

1459. González-Romero, M.E., Rivera, C., Cancino, K., Geu-Flores, F., Cosio, E.G., Ghislain, M. & Halkier. B.A. (2021). Bioengineering potato plants to produce benzylglucosinolate
for improved broad-spectrum pest and disease resistance. Transgenic Res. doi: 10.1007/s11248-021-00255-w. Online ahead of print.

1458. Hudson, A.M., Szabo, N.L., Loughran, G., Wills, N.M., Atkins, J.F. & Cooley, L. (2021). Tissue-specific dynamic codon redefinition in Drosophila. Proc. Natl. Acad. Sci. USA. 118(5):e2012793118..

1457. Jang, H., Park, Y.H., Kim, M-H., You, J., Ko, J-H, & Lee, J.T. (2021). Surface characteristics of porous carbon derived from genetically designed transgenic hybrid poplar for electric double-layer capacitors. App. Surface Sci. 545:148978.

1456. Jeong, Y,S., Ku, H-K., Jung, Y-J., Kim, J.K., Lee, K.B., Kim, J-K., Lim, S-H., Lee, D. & Ha, S.H. (2021). 2A-linked bi-, tri-, and quad-cistrons for the stepwise biosynthesis of ß-carotene, zeaxanthin, and ketocarotenoids in rice endosperm. Metab. Eng. Commun. 12:e00166.

1455. Lo, Y-T., Tulloch, F., Wu, H.C., Luke, G.A., Ryan, M.D. & Chu, C-Y. (2021). Expression and immunogenicity of secreted forms of bovine ephemeral fever virus glycoproteins applied to subunit vaccine development. J. Appl. Microbiol. Feb 18. doi: 10.1111/jam.15044. Online ahead of print.

1454. Meas, S., Mekvichitsaeng, P. & Roshorm, Y.M. (2021). Co-expression of self-cleaved multiple proteins derived from Porcine Reproductive and Respiratory Syndrome Virus by bi-cistronic and tri-cistronic DNA vaccines. Protein Expr. Purif. 177:105763.

1453. Müller, J.P., Scholl, S., Kunick, C. & Klempnauer, K.H. (2021). Expression of protein kinase HIPK2 is subject to a quality control mechanism that acts during translation and requires its kinase activity to prevent degradation of nascent HIPK2. Biochim. Biophys. Acta Mol. Cel.l Res. 1868(1):118894.

1452. Rand, U., Kubsch, T., Kasmapour, B. and Cicin-Sain, L. (2021). A novel triple-fluorescent HCMV strain reveals gene expression dynamics and anti-herpesviral drug mechanisms. Front. Cell Infect. Microbiol. 10, 536150.

1451. Rozen-Gagnon, K., Yi, S., Jacobson, E., Novack, S. & Rice, C.M. (2021). A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines. Sci Rep. 11(1):736.

1450. Sádlová, J., Podešvová, L., Becvár, T., Bianchi, C., Gerasimov, E.S., Saura, A., Glanzová, K., Leštinová, T., Matveeva, N.S., Chmelová, L., Mlacovská, D., Spitzová, T., Vojtková, B., Volf, P., Yurchenko, V. & Kraeva, N. (2021). Catalase impairs Leishmania mexicana development and virulence. Virulence 12, 852-867.

1449. Scarborough. A.M., Flaherty, J.N., Hunter, O.V., Liu, K., Kumar, A., Xing, C., Tu, B.P. & Conrad, N.K. (2021). SAM homeostasis is regulated by CFI(m)-mediated splicing of MAT2A. eLife 10:e64930.

1448. Shibata, A.C.E., Ueda, H.H., Eto, K., Onda, M., Sato, A., Ohba, T., Nabekura, J. & Murakoshi, H. (2021). Photoactivatable CaMKII induces synaptic plasticity in single synapses. Nat. Commun. 12(1):751.

1447. Shao, Q., Jia, X., Gao, Y., Liu, Z., Zhang, H., Tan, Q., Zhang, X., Zhou, H., Li, Y., Wu, D. & Zhang, Q. (2021). Cryo-EM reveals a previously unrecognized structural protein of a dsRNA virus implicated in its extracellular transmission. PLoS Pathog. 17(3):e1009396.

1446. Tasker, C., Patel, J., Jawa, V. & Maamary, J. (2021). Competition-based cell assay employing soluble T cell receptors to assess MHC class II antigen processing and presentation. AAPS J. 23(2):26.

1445. Wang, M., Mou, C., Chen, M. & Chen, Z. (2021). Infectious recombinant Senecavirus A expressing novel reporter proteins. Appl. Microbiol. Biotechnol. 105, 2385-2397.

1444. Work, J.J & Brandman, O. (2021). Adaptability of the ubiquitin-proteasome system to proteolytic and folding stressors. J. Cell. Biol. 220:e201912041.

1443. Xiong, Z., Lo, H.P., McMahon, K.A., Martel, N., Jones, A., Hill, M.M., Parton, R.G. & Hall, T.E. (2021). In vivo proteomic mapping through GFP-directed proximity-dependent biotin labelling in zebrafish. eLife 10:e64631.

1442. Yasuda, T., Saito,Y., Ono, C., Kawata, K., Baba, A. & Baba, Y. (2021). Generation and characterization of CD19-iCre mice as a tool for efficient and specific conditional gene targeting in B cells. Sci. Rep. 11:5524

1441. Zhang, H., Zheng, Q. & Chen-Tsai, R.Y. (2021). Establishment of a Cre-rat resource for creating conditional and physiological relevant models of human diseases. Transgenic Res. 30, 91-104.


2020

1440. Alarcón, H., Bonzon-Kulichenko, E., Peinado, R., Lim, F., Vázquez, J. & Rodríguez, A. (2020). Generation of a lentiviral vector system to efficiently express bioactive recombinant human prolactin hormones. Mol. Cell. Endocrinol. 499, 110605.

1439. Ando, R., Sakaue-Sawano, A., Shoda, K., Miyawaki, A., 2020. Two new coral fluorescent proteins of distinct colors for sharp visualization of cell-cycle progression. bioRxiv, 6.
https://doi.org/10.1101/2020.03.30.015156, 2020.03.30.01515.

1438. Arevalo-Villalobos, J., Govea-Alonso, D., Bañuelos-Hernández, B., González-Ortega, O., Zarazúa, S. & Rosales-Mendoza, S. (2020). Inducible expression of antigens in plants: a study focused on peptides related to multiple sclerosis immunotherapy. J. Biotechnol. 318, 51-56.

1437. Barakate, A., Keir, E., Oakey, H. & Halpin, C. (2020). Stimulation of homologous recombination in plants expressing heterologous recombinases. BMC Plant Biology 20, 336.

1436. Bohlender, L.L., Parsons, J., Hoernstein, S.N.W., Rempfer, C., Ruiz-Molina, N., Lorenz, T., Rodríguez Jahnke, F., Figl, R., Fode, B., Altmann, F., Reski, R. & Decker, E.L. (2020). Stable Protein Sialylation in Physcomitrella. Front. Plant Sci. 11:610032.

1435. Cai, L., Hu, H., Duan, H., Li, Y., Zou, Z., Luo, K., Zhang, Z., Yang, J., Jin, J., Chen, Y., Ke, Z., Fang, Z., Liu, Q., Hong, X., Hu, S. & Liu, B. (2020). The construction of a new oncolytic herpes simplex virus expressing murine interleukin-15 with gene-editing technology. J. Med. Virol. doi: 10.1002/jmv.25691. Online ahead of print.

1434. Chiale, C., Yarovinsky, T.O., Mason, S.W., Madina, B.R., Menon, M., Krady, M.M., Moshkani, S., Chattopadhyay Pal, A., Almassian, B., Rose, J.K., Robek, M.D. & Nakaar, V. (2020). Modified alphavirus-vesiculovirus hybrid vaccine vectors for homologous prime-boost immunotherapy of chronic hepatitis B. Vaccines (Basel) 8(2):279.

1433. Chu, I-R., Pan, R-L., Yang, C-S. and Wu, L-C. (2020). A doxycycline-inducible C17.2 neural stem cell-based combination of differentiation and suicide gene therapy for an in vitro tumorigenic C6 glioma model. Biotech. Biotechnological Equipment 34, 986-998.

1432. Costello, A., Lao, N.T., Barron, N. & Clynes, M. (2020). Reinventing the wheel: synthetic circular RNAs for mammalian cell engineering. Trends Biotechnol. 38, 217-230.

1431. Crowther, M, D., Dolton, G., Legut, M., Caillaud, M, E., Lloyd, A., Attaf, M., Galloway, S., Rius, C., Farrell, C, P., Szomolay, B., Ager, A., Parker, A, L., Fuller, A., Donia, M., McCluskey, J., Rossjohn, J., Svane, I, M., Phillips, J, D. & Sewell, A, K. (2020). Genome-wide CRISPR-Cas9 screening reveals ubiquitous T cell cancer targeting via the monomorphic MHC class I-related protein MR1. Nat. Immunol. 21,178-185.

1430. Cruz-Teran, C., Bacon, K. & Rao, BM. Eds: Zielonka, S. & Krah, S. (2020). Simultaneous soluble secretion and surface display of proteins in saccharomyces cerevisiae using inefficient pibosomal Sskipping. Genotype Phenotype Coupling: Methods And Protocols 2070, 321-334.

1429. Davola, M, E., Vito, A., Wei, J., El-Sayes, N., Workenhe, S. & Mossman, K, L. Eds: Galluzzi, L. & Rudqvist, N, P. (2020). Genetic modification of oncolytic viruses to enhance antitumor immunity. Tumor Immunology And Immunotherapy - Integrated Methods 635, 231-250.

1428. Dehghani, J., Adibkia, K., Movafeghi, A., Pourseif, M, M. & Omidi, Y. (2020). Designing a new generation of expression toolkits for engineering of green microalgae; robust production of human interleukin-2. Bioimpacts 10, 257-266.

1427. Del Papa, J., Clarkin, R, G. & Parks, R, J. (2020). Use of cell fusion proteins to enhance adenoviral vector efficacy as an anti-cancer therapeutic. Cancer Gene Therapy 2020 Jul 1. doi: 10.1038/s41417-020-0192-9

1426. Dhamija, S., Yang, C.M., Seiler, J., Myacheva, K., Caudron-Herger, M., Wieland, A., Abdelkarim, M., Sharma, Y., Riester, M., Groß, M., Maurer, J. & Diederichs, S. (2020). A pan-cancer analysis reveals nonstop extension mutations causing SMAD4 tumour suppressor degradation. Nat. Cell Biol. 22, 999-1010.

1425. Dorrington, R.A, Jiwaji, M., Awando, J.A & de Bruyn, M-M. (2020). Advances in Tetravirus research: new insight into the infectious virus life Cycle and an expanding host range. Curr. Issues Mol. Biol. 34, 145-162.

1424. El Fekih, S., Tous, C., Gueganic, N., Brugnon, F., Ben Ali, H., Bujan, L., Moinard, N., Caire-Tetauru, E., Ajina, M., Douet-Guilbert, N., Morel, F. & Perrin, A. (2020). Decrease of spermatozoa with an unbalanced chromosome content after cell sorting in men carrying a structural chromosomal abnormality. Andrology 8(1), 181-190.

1423. Farnworth, M, S., Eckermann, K, N., Ahmed, K, M., Mühlen, D, S., He, B. & Bucher, G. (2020). The red flour beetle as model for comparative neural development: Genome editing to mark neural cells in tribolium brain development. Methods Mol Biol. 2047, 191-217.

1422. Farrera-Sal, M., de Sostoa, J., Nuñez-Manchón, E., Moreno, R., Fillat, C., Bazan-Peregrino, M. & Alemany, R. (2020). Arming oncolytic adenoviruses: Effect of insertion site and splice acceptor on transgene expression and viral fitness. Int. J. Mol. Sci. 21(14):5158.

1421. Farrera-Sal, M., Fillat, C. & Alemany, R. (2020). Effect of transgene location, transcriptional control elements and transgene features in armed oncolytic adenoviruses. Cancers (Basel). 12(4):1034.

1420. Fuller, J., Álvarez-Rodríguez, B., Todd, E., Mankouri, J., Hewson, R. & Barr, J, N. (2020). Hazara Nairovirus Requires COPI Components in both Arf1-Dependent and Arf1-Independent Stages of Its Replication Cycle. J. Virol. 94, e00766-20.

1419. Garcia-Gonzalez, I., Mühleder, S., Fernández-Chacón, M. & Benedito, R. (2020). Genetic tools to study cardiovascular biology. Front Physiol. 11:1084.

1418. Gioseffi, A., Hamerly, T., Van, K., Zhang, N., Dinglasan, R.R., Yates, P.A. & Kima, P.E. (2020). Leishmania-infected macrophages release extracellular vesicles that can promote lesion development. Life Sci Alliance 3(12):e202000742..

1417. Guseva, S., Milles, S., Jensen, M, R., Kleman, J., Maurin, D., Ruigrok, R. & Blackledge, M. (2020). Measles virus nucleo- and phosphoproteins form liquid-like phase-separated compartments that promote nucleocapsid assembly. Sci Adv. 6, eaaz7095.

1416. Hao, J., Yang, X., Zhang, C., Zhang, X.T., Shi, M., Wang, S.H., Mi, L., Zhao, Y.T., Cao, H. & Wang, Y. (2020). KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells. Cell Prolif. 53(11):e12914.

1415. Han, P., Shichino, Y., Schneider-Poetsch, T., Mito, M., Hashimoto, S., Udagawa, T., Kohno, K., Yoshida, M., Mishima, Y., Inada, T. & Iwasaki, S. (2020). Genome-wide Survey of Ribosome Collision. Cell Reports. 31, (5).

1414. Hannemann, H. (2020). Viral replicons as valuable tools for drug discovery. Drug Discov. Today. 25, 1026-1033.

1413. He, Y., Zhang, T., Sun, H., Zhan, H. & Zhao, Y. (2020). A reporter for noninvasively monitoring gene expression and plant transformation. Hortic. Res. 7:152.

1412. Hinz, S, C., Elter, A., Grzeschik, J., Habermann, J., Becker, B. & Kolmar, H. (2020). Simplifying the detection of surface presentation levels in yeast surface display by intracellular tgfp expression. Methods Mol. Biol. 211-222.

1411. Huang, J., Wang, A., Huang, C., Sun, Y., Song, B., Zhou, R. & Li, L. (2020). Generation of marker-free pbd-2 knock-in pigs using the CRISPR/Cas9 and Cre/loxP systems. Genes (Basel) 11(8):951.

1410. Ibrahim, S., Harris-Kawano, A., Haider, I., Mirmira, R, G., Sims, E, K. & Anderson, R, M. (2020). A novel Cre-enabled tetracycline-inducible transgenic system for tissue-specific cytokine expression in the zebrafish: CETI-PIC3. Dis. Model Mech. 13(6).

1409. Jaffri, S.A., Yan, Y., Schwirz, J. & Schetelig, M.F. (2020). Functional characterization of the Drosophila suzukii pro-apoptotic genes reaper, head involution defective and grim. Apoptosis 25, 864-874.

1408. Ji, Y., Li, B., Qiao, M., Li, J., Xu, H., Zhang, L. & Zhang, X. (2020). Advances on the in vivo and in vitro glycosylations of flavonoids. Appl. Microbiol. Biotechnol. 104, 6587-6600.

1407. Juszkiewicz, S., Slodkowicz, G., Lin, Z., Freire-Pritchett, P., Peak-Chew, S.Y. & Hegde, R.S. (2020). Ribosome collisions trigger cis-acting feedback inhibition of translation initiation. eLife 9:e60038.

1406. Khosla, A. & Nelson, D.C. (2020). Ratiometric measurement of protein abundance after transient expression of a transgene in Nicotiana benthamiana. BioProtoc. 10(17):e3747.

1405. Kelly, F.D., Tran, K.D., Hatfield, J., Schmidt, K., Sanchez, M.A. and Landfear, S.M. (2020). A cytoskeletal protein complex is essential for division of intracellular amastigotes of Leishmania mexicana. J. Bio.l Chem. 295, 13106-13122.

1404. Khosla, A., Rodriguez-Furlan, C., Kapoor, S, J., Van Norman, J. & Nelson, D, C. (2020). A series of dual-reporter vectors for ratiometric analysis of protein abundance in plants. Plant Direct. 4, e00231.

1403. Khosla, A., Morffy, N., Li, Q., Faure, L., Chang, S.H., Yao, J., Zheng, J., Cai, M.L., Stanga, J., Flematti, G.R., Waters, M.T. & Nelson, D.C. (2020). Structure-Function Analysis of SMAX1 reveals domains that mediate its Karrikin-induced proteolysis and interaction with the receptor KAI2. Plant Cell 32, 2639-2659.

1402. Kojin, B, B., Biedler, J, K., Tu, Z. & Adelman, Z, N. (2020). Characterization of a female germline and early zygote promoter from the transcription factor bZip1 in the dengue mosquito Aedes aegypti. Parasit. Vectors 13, 353.

1401. Komoto, S., Fukuda, S., Hatazawa, R., Murata, T. & Taniguchi, K. (2020). Generation of recombinant rotaviruses from just 11 cDNAs encoding a viral genome. Virus Res. 286:198075.

1400. Kondo, S., Takahashi, T., Yamagata, N., Imanishi, Y., Katow, H., Hiramatsu, S., Lynn, K., Abe, A., Kumaraswamy, A. & Tanimoto, H. (2020). Neurochemical organization of the drosophila brain visualized by endogenously tagged neurotransmitter receptors. Cell Rep. 30, 284-297.

1399. Krah, S., Günther, S., Becker, S., Zielonka, S. & Rhiel, L. (2020). Chemical modification of the yeast cell surface allows the switch between display and soluble secretion of full-length antibodies. Methods in Microbiology 2070, 335-349.

1398. Krishnamurthy, V, V., Fu, J., Oh, T., Khamo, J., Yang, J. & Zhang, K. (2020). A generalizable optogenetic strategy to regulate receptor tyrosine kinases during vertebrate embryonic development. J. Mol. Biol. 432, 3149-3158.

1397. Kropp, K.A., Srivaratharajan, S., Ritter, B., Yu, P., Krooss, S., Polten, F., Pich, A., Alcami, A. & Viejo-Borbolla, A. (2020). Identification of the cleavage domain within glycoprotein G of Herpes Simplex Virus Type 2. Viruses 12(12):1428.

1396. Lee, J.H., Won, H.J., Oh, E.S., Oh, M.H. & Jung, J.H. (2020). Golden gate cloning-compatible DNA replicon/2A-mediated polycistronic vectors for plants. Front. Plant Sci. 11:559365.

1395. Levin, S, D., Evans, L, S., Bort, S., Rickel, E., Lewis, K, E., Wu, R, P., Hoover, J., MacNeil, S., La, D., Wolfson, D, F., Rixon, M, W., Dillon, S, R., Kornacker, M, G., Swanson, R. & Peng, S, L. (2020). Novel immunomodulatory proteins generated via directed evolution of variant IgSF domains. Front. Immunol. 10, 3086.

1394. Li, H., Zhang, Q., Gu, Y., Wu, Y., Wang, Y., Wang, L., Feng, S., Hu, Y., Zheng, Y., Li, Y., Ye, H., Zhou, B., Lin, L., Liu, M., Yang, H. & Li, D. (2020). Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse. Proc. Natl. Acad. Sci. USA. 117, 33426-33435.

1393. Li, X., Bi, Z., Sun, Y., Li, C., Li, Y. & Liu, Z. (2020). In vivo ectopic Ngn1 and Neurod1 convert neonatal cochlear glial cells into spiral ganglion neurons. FASEB J. 34, 4764-4782.

1392. Li, Y.M., Wang, M., Wang, T.Y., Wei, Y.G., Guo, X., Mi, C.L., Zhao, C.P., Cao, X.X. & Dou, Y.Y. (2020). Effects of different 2A peptides on transgene expression mediated by tricistronic vectors in transfected CHO cells. Mol. Biol. Rep. 47, 469-475.

1391. Licon, M, H. & Yates, P, A. (2020). Purine-responsive expression of the Leishmania donovani NT3 purine nucleobase transporter is mediated by a conserved RNA stem-loop. J. Biochem. 25, 8449-8459.

1390. Lin, Y., Weng, K., Liang, J., Zhou, Y., Hao, Q., Hao, Y., Yao, K. & Zou, J. (2020). The extracellular and intracellular regions of Crb2a play distinct roles in guiding the formation of the apical zonula adherens. Biomed. Pharmacother. 125, 109942.1382.

1389. Lin, Y.Y., Schuphan, J., Dickmeis, C., Buhl, E.M., Commandeur, U., Fischer, H. (2020). Attachment of ultralow amount of engineered plant viral nanoparticles to mesenchymal stem cells enhances osteogenesis and mineralization. Adv. Healthc. Mater. 9(21):e2001245..

1388. Matreyek, K.A., Stephany, J.J., Chiasson, M.A., Hasle, N. & Fowler, D.M. (2020). An improved platform for functional assessment of large protein libraries in mammalian cells. Nuc. Acids Res. 48(1):e1.

1387. Matsunaga, S., Jeremiah, S, S., Miyakawa, K., Kurotaki, D., Shizukuishi, S., Watashi, K., Nishitsuji, H., Kimura, H., Tamura, T., Yamamoto, N., Shimotohno, K., Wakita, T. & Ryo, A. (2020). Engineering cellular biosensors with customizable antiviral responses targeting Hepatitis B Virus. iScience 23:100867.

1386. Mizote, Y., Masumi-Koizumi, K., Katsuda, T. & Yamaji, H. (2020). Production of an antibody Fab fragment using 2A peptide in insect cells. J. Biosci. Bioeng. 130, 205-211.

1385. Mizushima, R., Inoue, K., Fujiwara, H., Iwane, H., Watanabe, T, M. & Kimura, A. (2020). Multiplexed 129 Xe hyperCEST MRI detection of genetically reconstituted bacterial protein nanoparticles in human cancer cells. Contrast Media & Molecular Imaging. 5425934.

1384. Molina-Márquez, A., Vila, M., Rengel, R., Fernández, E., García-Maroto, F., Vigara, J. & León, R. (2020). Validation of a new multicistronic plasmid for the efficient and stable expression of transgenes in microalgae. Int. J. Mol. Sci. 21, 718.

1383. Morgan, R, A., Ma, F., Unti, M, J., Brown, D., Ayoub, P, G., Tam, C., Lathrop, L., Aleshe, B., Kurita, R., Nakamura, Y., Senadheera, S., Wong, R, L., Hollis, R, P., Pellegrini, M. & Kohn, B, D. (2020). Creating new ß-globin-expressing lentiviral vectors by high-resolution mapping of locus control region enhancer sequence. Mol. Ther. Methods Clin. Dev. 18, 999-1013.

1382. Mu, C., Vakharia, V.N., Zhou, Y., Jiang, N., Liu, W., Meng, Y., Li, Y., Xue, M., Zhang, J., Zeng, L., Zhong, Q. and Fan, Y. (2020). A novel subunit vaccine based on outer capsid proteins of Grass Carp Reovirus (GCRV) provides protective immunity against GCRV infection in Rare Minnow (Gobiocypris rarus). Pathogens 9, 945.

1381. Muhammad, B. B., Yusoff, K., Ideris, A., Hair-Bejo, M., Jibril, A., Peeters, B. & Omar, A. (2020). Exploring the prospects of engineered Newcastle Disease Virus in modern vaccinology. Viruses 12(4):451.

1380. Müntjes, K., Philipp, M., Hüsemann, L., Heucken, N., Weidtkamp-Peters, S., Schipper, K., Zurbriggen, M, D. & Feldbrügge, M. (2020). Establishing polycistronic expression in the model microorganism Ustilago maydis. Front. Microbiol. 11,1384.

1379. Murakoshi, H. (2021). Optogenetic imaging of protein activity using two-photon fluorescence lifetime imaging microscopy. Adv. Exp. Med. Biol. 1293, 295-308.

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1273. Salehi Sangani, G., Jajarmi, V., Khamesipour, A., Mahmoudi, M., Fata, A. & Mohebali, M. (2019). Generation of a CRISPR/Cas9-based vector specific for gene manipulation in Leishmania major. Iran J. Parasitol. 14, 78-88.

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1269. Shibuta, M.K., Matsuoka, M. & Matsunaga, S. (2019). 2A Peptides contribute to the co-expression of proteins for imaging and genome editing. Cytologia 84, 107-111.

1268. Sitron, C.S. & Brandman, O. (2019). CAT tails drive degradation of stalled polypeptides on and off the ribosome. Nat. Struct. Mol. Biol. 26(6):450-459.

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1264. Tang, F., Yang, X., Liu, D., Zhang, X., Huang, X., He, X., Shi, J., Li, Z. & Wu, Z. (2019). Co-expression of fat1 and fat2 in transgenic pigs promotes synthesis of polyunsaturated fatty acids.Transgenic Res. 28, 369-379.

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1262. Tian, Y., Li, Y., Zhao, F. & Meng, C. (2019). Engineered Pichia pastoris production of fusaruside, a selective immunomodulator. BMC Biotechnol. 19(1):37. doi: 10.1186/s12896-019-0532-8.

1261. Tzin, V., Snyder, J.H., Yang, D.S., Huhman, D.V., Watson, B.S., Allen, S.N., Tang, Y., Miettinen, K., Arendt, P., Pollier, J., Goossens, A. & Sumner, L.W. (2019). Integrated metabolomics identifies CYP72A67 and CYP72A68 oxidases in the biosynthesis of Medicago truncatula oleanate sapogenins. Metabolomics 15(6):85. doi: 10.1007/s11306-019-1542-1.

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1240. Aznar, A., Chalvin, C., Shih, P.M., Maimann, M., Ebert, B., Birdseye, D., Loqué, D., Scheller, H. (2018). Gene stacking of multiple traits for high yield of fermentable sugars in plant biomass. Biotechnol Biofuels. 11:2. doi: 10.1186/s13068-017-1007-6. eCollection 2018.

1239. Bergiers, I., Andrews, T., Vargel Bölükbasi, Ö., Buness, A., Janosz, E., Lopez-Anguita, N., Ganter, K., Kosim, K., Celen, C., Itir Perçin, G., Collier, P., Baying, B., Benes, V., Hemberg, M. & Lancrin, C. (2018). Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis. Elife. 20;7. pii: e29312.

1238. Berlin, S. & Isacoff, E.Y. (2018). Optical control of glutamate receptors of the NMDA-kind in mammalian neurons, with the use of photoswitchable ligands. In ‘Biochemical Approaches For Glutamatergic Neurotransmission’. Parrot, S. & Denoroy, L. (eds). 130, 293-325.

1237. Boesmans, W., Hao, M.M. & Vanden, Berghe, P. (2018). Optogenetic and chemogenetic techniques for neurogastroenterology. Nat. Rev. Gastroenterol. Hepatol. 15, 21-38.

1236. Boonyalai, N., Collins, C.R., Hackett, F., Withers-Martinez, C. & Blackman, M.J. (2018). Essentiality of Plasmodium falciparum plasmepsin V. PLoS One. 13(12):e0207621.

1235. Cai, H., Liu, M. & Russell, C.J. (2018). Directed evolution of an influenza reporter virus to restore replication and virulence and enhance non-invasive bioluminescence imaging in mice. J. Virol. 2018 Jun 13. pii: JVI.00593-18. doi: 10.1128/JVI.00593-18. [Epub ahead of print]

1234. Chen, A.Y. & Tully, T. (2018). A stress-enhanced model for discovery of disease-modifying gene: Ece1-suppresses the toxicity of a-synuclein A30P. Neurobiol Dis. 114, 153-163.

1233. Chitwood, P.J., Juszkiewicz, S., Guna, A., Shao, S. & Hegde, R.S. (2018). EMC is required to initiate accurate membrane protein topogenesis. Cell 175, 1507-1519.

1232. Cieri, D., Vicario, M., Giacomello, M., Vallese, F., Filadi, R., Wagner, T., Pozzan, T., Pizzo, P., Scorrano, L., Brini, M. & Calì, T. (2018). SPLICS: a split green fluorescent protein-based contact site sensor for narrow and wide heterotypic organelle juxtaposition. Cell Death Differ. 25, 131-1145.

1231. Collmann, F.M., Pijnenburg, R., Schneider, G., Schäfer, C., Folz-Donahue, K., Kukat, C. & Hoehn, M. (2018). Imaging reporter strategy to monitor gene activation of microglia polarisation states under stimulation. J. Neuroimmune Pharmacol. 13, 371-382.

1230. Conrad, S.J., Silva, R.F., Hearn, C.J., Climans, M. & Dunn, J.R. (2018). Attenuation of Marek's disease virus by codon pair deoptimization of a core gene. Virology 516, 219-226.

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1227. Chen, A.Y. & Tully, T. (2018). A stress-enhanced model for discovery of disease-modifying gene: Ece1-suppresses the toxicity of a-synuclein A30P. Neurobiol Dis. 114, 153-163.

1226. Chen, Y., Song, T., Xiao, Y.L., Wan, X., Yang, L., Li, J., Zeng, G., Fang, P., Wang, Z.Z. & Gao, R. (2018). Enhancement of immune response of piglets to PCV-2 vaccine by porcine IL-2 and fusion IL-4/6 gene entrapped in chitosan nanoparticles. Res. Vet. Sci. 117, 224-232.

1225. Chitwood, P.J., Juszkiewicz, S., Guna, A., Shao, S. & Hegde, R.S. (2018). EMC is required to initiate accurate membrane protein topogenesis. Cell 175, 1507-1519.

1224. Conrad, S.J., Silva, R.F., Hearn, C.J., Climans, M. & Dunn, J.R. (2018). Attenuation of Marek's disease virus by codon pair deoptimization of a core gene. Virology 516, 219-226.

1223. Dai, K.Z., Ryan, J.C., Naper, C., Vaage, J.T. (2018). Identification of MHC Class Ib ligands for stimulatory and inhibitory Ly49 receptors and induction of potent NK cell alloresponses in rats. J. Immunol. 200, 2847-2859.

1222. DelBove, C.E., Deng, X.Z. & Zhang, Q. (2018). The fate of nascent APP in hippocampal neurons: a live cell imaging study. ACS Chem Neurosci. 9, 2225-2232.

1221. Deng, H., Gao, R., Liao, X., & Cai, Y. (2018). Characterisation of a monooxygenase in Shiraia bambusicola. Microbiology 164, 1180-1188.

1220. Dranchak, P., Moran, J.J., MacArthur, R., Lopez-Anido, C., Inglese, J. & Svaren, J. (2018). Genome-edited cell lines for high-throughput screening. Methods Mol Biol. 1755:1-17. doi: 10.1007/978-1-4939-7724-6_1.

1219. Fastman, Y., Assaraf, S., Rose, M., Milrot, E., Basore, K., Arasu, B.S., Desai, S.A., Elbaum, M. & Dzikowski, R (2018). An upstream open reading frame (uORF) signals for cellular localization of the virulence factor implicated in pregnancy associated malaria. Nucleic Acids Res. 46, 4919-4932.

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1215. Gunišová, S., Hronová, V., Mohammad, M.P., Hinnebusch, A.G. & Valášek, L.S. (2018). Please do not recycle! Translation reinitiation in microbes and higher eukaryotes. FEMS Microbiol. Rev. 42, 165-192.

1214. Gawdzik, J.C., Yue, M.S., Martin, N.R., Elemans, L.M.H., Lanham, K.A., Heideman, W., Rezendes, R., Baker, T.R., Taylor, M.R. & Plavicki, J.S. (2018). sox9b is required in cardiomyocytes for cardiac morphogenesis and function. Sci. Rep. 8(1):13906. doi: 10.1038/s41598-018-32125-7.

1213. Hadpech, S., Jinathep, W., Saoin, S., Thongkum, W., Chupradit, K., Yasamut, U., Moonmuang, S. & Tayapiwatana, C. (2018). Impairment of a membrane-targeting protein translated from a downstream gene of a "self-cleaving" T2A peptide conjunction. Protein Expr. Purif. 150, 17-25.

1212. Hale, A.J., den Hertog, J. (2018). Shp2-mitogen-activated protein kinase signaling drives proliferation during zebrafish embryo caudal fin fold regeneration. Mol Cell Biol. 38(4). pii: e00515-17.

1211. Hettige, N.C., Manzano-Vargas, K., Jefri, M. & Ernst, C. (2018). Strategies to advance drug discovery in rare monogenic intellectual disability syndromes. Int. J. Neuropsychopharmacol. 21, 201-206.

1210. Hisano, S., Zhang, R., Faruk, I, M., Kondo, H. & Suzuki, N. (2018). A neo-virus lifestyle exhibited by a (+)ssRNA virus hosted in an unrelated dsRNA virus: Taxonomic and evolutionary considerations. Virus Research 15, 75-83.

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1208. Hofacre, A., Yagiz, K., Mendoza, D., Lopez Espinoza, F., Munday, A.W., Burrascano, C., Singer, O., Gruber, H.E., Jolly, D.J. & Lin, A.H. (2018). Efficient therapeutic protein expression using retroviral replicating vector with 2A peptide in cancer models. Hum. Gene Ther. 29, 437-451.

1207. Hou, G., Li, J., Wang, Y., Wang, S., Peng, C., Yu, X., Jin, J. & Jiang, W. (2018). Influenza viral vectors expressing two kinds of HA proteins for bivalent vaccines against clade 2.3.4.4 and clade 2.3.2.1 H5 HPAIVs. Sci. Rep. 8(1):9327. doi: 10.1038/s41598-018-27722-5.

1206. Husa, A.M., Strobl, M.R., Strajeriu, A., Wieser, M., Strehl, S. & Fortschegger, K. (2018). Generation of CD34 fluorescent reporter human induced pluripotent stem cells for monitoring hematopoietic differentiation. Stem Cells Dev. 27, 1376-1384.

1205. Inderberg, E.M., Mensali, N., Oksvold, M.P., Fallang, L.E., Fåne, A., Skorstad, G., Stenvik, G.E., Progida, C., Bakke, O., Kvalheim, G., Myklebust, J.H. & Wälchli, S. (2018). Human c-SRC kinase (CSK) overexpression makes T cells dummy. Cancer Immunol Immunother. 67, 525-536.

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1182. Milles, S., Jensen, M, R., Lazert, C., Guseva, S., Ivashchenko, S., Communie, G., Maurin, D., Gerlier, D., Ruigrok, R. & Blackledge, M. (2018). An ultraweak interaction in the intrinsically disordered replication machinery is essential for measles virus function. Sci Adv. 4(8),eaat7778.

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1176. Park, S-W., Do, H-J., Choi, W., Kim, H.J., Kang, M-J., Seo, H.G. & Kim, J-H. (2018). CRISPR/Cas9 knock-in of GST-tagged human Noggin in the beta-casein gene locus of bovine ear fibroblast cells. Indian J. Biotech. 17, 553-560.

1175. Pennell, C.A., Barnum, J.L., McDonald-Hyman, C.S., Panoskaltsis-Mortari, A., Riddle, M.J., Xiong, Z., Loschi, M., Thangavelu, G., Campbell, H.M., Storlie, M.D., Refaeli, Y., Furlan, S.N., Jensen, M.C., Kean, L.S., Miller, J.S., Tolar, J., Osborn, M.J. & Blazar B.R. (2018). Human CD19-targeted mouse T cells induce B cell aplasia and toxicity in human CD19 transgenic mice. Mol Ther. 26, 1423-1434.

1174. Poliner, E., Pulman, J.A., Zienkiewicz, K., Childs, K., Benning, C. & Farré, E.M. (2018). A toolkit for Nannochloropsis oceanica CCMP1779 enables gene stacking and genetic engineering of the eicosapentaenoic acid pathway for enhanced long-chain polyunsaturated fatty acid production. Plant Biotechnol J. 16, 298-309.

1173. Ponciano, G., Dong, N., Chen, G. & McMahan, C. (2018). A bicistronic transgene system for genetic modification of Parthenium argentatum. Plant Biotech. Rep. 12, 149-155.

1172. Poulin, J.F., Caronia, G., Hofer, C., Cui, Q., Helm, B., Ramakrishnan, C., Chan, C.S., Dombeck, D.A., Deisseroth, K. & Awatramani, R. (2018). Mapping projections of molecularly defined dopamine neuron subtypes using intersectional genetic approaches. Nat. Neurosci. 21, 1260-1271.

1171. Puckette, M., Smith, J.D., Gabbert, L., Schutta, C., Barrera, J., Clark, B.A., Neilan, J.G. & Rasmussen, M. (2018). Production of foot-and-mouth disease virus capsid proteins by the TEV protease. J. Biotechnol. 2018 Jun 10;275:7-12. doi: 10.1016/j.jbiotec.2018.03.012. Epub 2018 Mar 23.

1170. Qin, L., Jiang, X., Dong. Z., Huang, J. & Chen, X. (2018). Identification of two integration sites in favor of transgene expression in Trichoderma reesei. Biotechnol Biofuels. 11:142. doi: 10.1186/s13068-018-1139-3. eCollection 2018.

1169. Raje, H.S., Lieux, M.E. & DiMario, P.J. (2018). R1 retrotransposons in the nucleolar organizers of Drosophila melanogaster are transcribed by RNA polymerase I upon heat shock. Transcription 9, 273-285.

1168. Ranadheera, C., Proulx, R., Chaiyakul, M., Jones, S., Grolla, A., Leung, A., Rutherford, J., Kobasa, D., Carpenter, M. & Czub, M. (2018). The interaction between the Nipah virus nucleocapsid protein and phosphoprotein regulates virus replication. Sci Rep. 8,15994.

1167. Renaud-Gabardos, E., Tatin, F., Hantelys, F., Lebas, B., Calise, D., Kunduzova, O., Masri, B., Pujol, F., Sicard, P., Valet, P., Roncalli, J., Chaufour, X., Garmy-Susini, B., Parini, A. & Prats, A.C. (2018). Therapeutic benefit and gene network regulation by combined gene transfer of Apelin, FGF2, and SERCA2a into ischemic heart. Mol. Ther. 26, 902-916.

1166. Röder, J., Dickmeis, C., Fischer, R. & Commandeur, U. (2018). Systemic infection of Nicotiana benthamiana with potato virus X nanoparticles presenting a fluorescent iLOV polypeptide fused directly to the coat protein. Biomed. Res. Int. 2018 Feb 13;2018:9328671.

1165. Rosowski, S., Becker, S., Toleikis, L., Valldorf, B., Grzeschik, J., Demir, D., Willenbücher, I., Gaa, R., Kolmar, H., Zielonka, S. & Krah, S. (2018). A novel one-step approach for the construction of yeast surface display Fab antibody libraries. Microb Cell Fact. 17(1):3.

1164. Rustanti, L., Jin, H., Li, D., Lor, M., Sivakumaran, H. & Harrich, D. (2018). Differential effects of strategies to improve the transduction efficiency of lentiviral vector that conveys an anti-HIV protein, nullbasic, in human T cells. Virol Sin. 33, 142-152.

1163. Sánchez-Hernández, L., Hernández-Soto, J., Vergara, P., González, R.O. & Segovia, J. (2018). Additive effects of the combined expression of soluble forms of GAS1 and PTEN inhibiting glioblastoma growth. Gene Ther. 25, 439-449.

1162. Seeland, I., Xiong, Y., Orlik, C., Deibel, D., Prokosch, S., Küblbeck, G., Jahraus, B., De Stefano, D., Moos, S., Kurschus, F.C., Arnold, B. & Samstag, Y. (2018). The actin remodeling protein cofilin is crucial for thymic alpha beta but not gamma delta T-cell development. PLoS Biol. 16(7):e2005380. doi: 10.1371/journal.pbio.2005380. eCollection 2018 Jul.

1161. Shi, Y., Dincheva-Vogel, L., Ayemoba, C.E., Fung, J.P., Bergamaschi, C., Pavlakis, G.N., Farzaneh, F. & Gaensler, K.M.L. (2018). IL-15/IL-15Ra/CD80-expressing AML cell vaccines eradicate minimal residual disease in leukemic mice. Blood Adv. 2, 3177-3192.

1160. Shibata, H., Yasumi, T., Shimodera, S., Hiejima, E., Izawa, K., Kawai, T., Shirakawa, R., Wada, T., Nishikomori, R., Horiuchi, H., Ohara, O., Ishii, E. & Heike, T. (2018). Human CTL-based functional analysis shows the reliability of a munc13-4 protein expression assay for FHL3 diagnosis. Blood 131, 2016-2025.

1159. Siripong, W., Wolf, P., Kusumoputri, T.P., Downes, J.J., Kocharin, K., Tanapongpipat, S. & Runguphan, W. (2018). Metabolic engineering of Pichia pastoris for production of isobutanol and isobutyl acetate. Biotechnol Biofuels. 11:1.

1158. Souza-Moreira, T.M., Navarrete, C., Chen, X., Zanelli, C.F., Valentini, S.R., Furlan, M., Nielsen, J. & Krivoruchko, A. (2018). Screening of 2A peptides for polycistronic gene expression in yeast. FEMS Yeast Res. Aug 1;18(5). doi: 10.1093/femsyr/foy036.

1157. Stavrou, M., Philip, B., Traynor-White, C., Davis, C.G., Onuoha, S., Cordoba, S., Thomas, S. & Pule, M. (2018). A rapamycin-activated caspase 9-based suicide gene. Mol. Ther. 26, 1266-1276.

1156. Suphatrakul, A., Duangchinda, T., Jupatanakul, N., Prasittisa, K., Onnome, S., Pengon, J. & Siridechadilok, B. (2018). Multi-color fluorescent reporter dengue viruses with improved stability for analysis of a multi-virus infection. PLoS One 13(3):e0194399.

1155. Vo, T.K., Cho, J.S., Kim, S.S., Ko, J.H. & Kim, J., (2018). Genetically engineered hybrid poplars for the pyrolytic production of bio-oil: Pyrolysis characteristics and kinetics. Energy Conversion and Management 153, 48-59.

1154. Vujadinovic, M., Wunderlich, K., Callendret, B., Koning, M., Vermeulen, M., Sanders, B., van der Helm, E., Gecgel, A., Spek, D., de Boer, K., Stalknecht, M., Serroyen, J., Grazia Pau, M., Schuitemaker, H., Zahn, R., Custers, J. & Vellinga, J. (2018). Adenoviral type 35 and 26 vectors with a bidirectional expression cassette in the E1 region show an improved genetic stability profile and potent transgene-specific immune response. Hum. Gene Ther. 29, 337-351.

1153. Wang, Q., Fang, J., Pan, Q., Wang, Y., Xue, T., Li, L. & Chen, T. (2018). Efficient and stable delivery of multiple genes to fish cells by a modified recombinant baculovirus system. Int J Mol Sci. 19(12). pii: E3767.

1152. Wang, Z., Mehra, V., Simpson, M.T., Maunze, B., Chakraborty, A., Holan, L., Eastwood, E., Blackmore, M.G. & Venkatesh, I. (2018). KLF6 and STAT3 co-occupy regulatory DNA and functionally synergize to promote axon growth in CNS neurons. Sci Rep. 8:12565. doi: 10.1038/s41598-018-31101-5.

1151. Wen, W., Cheng, X., Fu, Y., Meng, F., Zhang, J.P., Zhang, L., Li, X.L., Yang, Z., Xu, J., Zhang, F., Botimer, G.D., Yuan, W., Sun, C., Cheng, T. & Zhang, X.B. (2018). High-level precise knockin of iPSCs by simultaneous reprogramming and genome editing of human peripheral blood mononuclear cells. Stem Cell Reports. 2018 Jun 5;10(6):1821-1834. doi: 10.1016/j.stemcr.2018.04.013. Epub 2018 May 10.

1150. White, K.M., Abreu, P. Jr., Wang, H., De Jesus, P.D., Manicassamy, B., García-Sastre, A., Chanda, S.K., DeVita, R.J. & Shaw, M.L. (2018). Broad spectrum inhibitor of influenza A and B viruses targeting the viral nucleoprotein. ACS Infect Dis. 4, 146-157.

1149. Wu, Q. & Bazzini, A.A. (2018). Systems to study codon effect on post-transcriptional regulation of gene expression. Methods. 2018 Mar 15;137:82-89. doi: 10.1016/j.ymeth.2017.11.006. Epub 2017 Nov 22.

1148. Wu, X., Liu, W. & Ding, H. (2018). A Pdgf-cCreERT2 knock-in mouse model for tracing PDGF-C cell lineages during development. Genesis 56(2). doi: 10.1002/dvg.23092. Epub 2018 Jan 31.

1147. Xue, Y., Chen, B., Win, A.N., Fu, C., Lian, J., Liu, X., Wang, R., Zhang, X. & Chai, Y. (2018). Omega-3 fatty acid desaturase gene family from two w-3 sources, Salvia hispanica and Perilla frutescens: Cloning, characterization and expression. PLoS One Jan 19;13(1):e0191432. doi: 10.1371/journal.pone.0191432. eCollection 2018.

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1145. Yang, Y., You, M., Chen, F., Jia, T., Chen, Y., Zhou, B., Mi, Q., An, Z., Luo, W. & Xia, N. (2018). Efficient development of a stable cell pool for antibody production using a single plasmid. J. Biochemistry 163, 391-398.

1144. Yordanova, M.M., Loughran, G., Zhdanov, A.V., Mariotti, M., Kiniry, S.J., O'Connor, P.B.F., Andreev, D.E., Tzani, I., Saffert, P., Michel, A.M., Gladyshev, V.N., Papkovsky, D.B., Atkins, J.F. & Baranov, P.V. (2018). AMD1 mRNA employs ribosome stalling as a mechanism for molecular memory formation. Nature 553, 356-360.

1143. Zaini, M.N., Patel, S.A., Syafruddin, S.E., Rodrigues, P. & Vanharanta, S. (2018). Endogenous HIF2A reporter systems for high-throughput functional screening. Sci. Rep. 8(1):12063. doi: 10.1038/s41598-018-30499-2.

1142. Zeng, L., Kortschak, R.D., Raison, J.M., Bertozzi, T. & Adelson, D.L. (2018). Superior ab initio identification, annotation and characterisation of TEs and segmental duplications from genome assemblies. PLoS One 13(3):e0193588.

1141. Zhang, K., Zhang, X., Cai, Z., Zhou, J., Cao, R., Zhao, Y., Chen, Z., Wang, D., Ruan, W., Zhao, Q., Liu, G., Xue, Y., Qin, Y., Zhou, B., Wu, L., Nilsen, T., Zhou, Y. & Fu X.D. (2018). A novel class of microRNA-recognition elements that function only within open reading frames. Nat. Struct. Mol. Biol. 25, 1019-1027.

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2017

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1138. Amarnath, S., Stevens, L.M. & Stein, D.S.(2017). Reconstitution of Torso signaling in cultured cells suggests a role for both Trunk and Torso-like in receptor activation. Development 144, 677-686.

1137. Armbruster, N., Krieg, J., Weißenberger, M., Scheller, C. & Steinert, A.F. (2017). Rescued chondrogenesis of mesenchymal stem cells under interleukin 1 challenge by foamyviral interleukin 1 receptor antagonist gene transfer. Front. Pharmacol. 8:255. doi: 10.3389/fphar.2017.00255.

1136. Askou, A.L., Benckendorff, J.N.E., Holmgaard, A., Storm, T., Aagaard, L., Bek, T., Mikkelsen, J.G. & Corydon, T.J. (2017). Suppression of choroidal neovascularization in mice by subretinal delivery of multigenic lentiviral vectors encoding anti-angiogenic microRNAs. Hum. Gene Ther. Methods. 28, 222-233.

1135. Beckmann, J.F., Ronau, J.A. & Hochstrasser, M. (2017). A Wolbachia deubiquitylating enzyme induces cytoplasmic incompatibility. Nat. Microbiol. 2017 Mar 1;2:17007. doi: 10.1038/nmicrobiol.2017.7.

1134. Biswal, J.K., Subramaniam, S., Ranjan, R. & Pattnaik, B. (2017). Uncleaved 2A-peptide of foot-and-mouth disease virus can display foreign epitope-tag at the virion surface. Infect Genet Evol. 54, 324-329.

1133. Brady, J.M., Baltimore, D. & Balazs, A.B. (2017). Antibody gene transfer with adeno-associated viral vectors as a method for HIV prevention. Immunol Rev. 275, 324-333.

1132. Chen, T.H., Hu, C.C., Liao, J.T., Lee, Y.L., Huang, Y.W., Lin, N.S., Lin, Y.L. & Hsu, Y.H. (2017). Production of Japanese Encephalitis Virus antigens in plants using Bamboo Mosaic Virus-based vector. Front. Microbiol. 8, 788. doi: 10.3389/fmicb.2017.00788.

1131. Chen, Y.H., Wu, K.J., Wu, K.L., Wu, K.L., Tsai, H.M., Chen, M.L., Chen, Y.W., Hsieh, W., Lin, C.M. & Wang, Y. (2017). Recombinant adeno-associated virus-mediated expression of methamphetamine antibody attenuates methamphetamine-induced hyperactivity in mice. Sci. Rep. 7:46301. doi: 10.1038/srep46301.

1130. Chisci, E., De Giorgi, M., Zanfrini, E., Testasecca, A., Brambilla, E., Cinti, A., Farina, L., Kutryb-Zajac, B., Bugarin, C., Villa, C., Grassilli, E., Combi, R., Gaipa, G., Cerrito, M.G., Rivolta, I., Smolenski, R.T., Lavitrano, M. & Giovannoni, R. (2017). Simultaneous overexpression of human E5NT and ENTPD1 protects porcine endothelial cells against H2O2-induced oxidative stress and cytotoxicity in vitro. Free Radic. Biol. Med. 108, 320-333.

1129. Choi, J., Park, S.Y., Hong, J., Kim, S. & Kim, Y.G. (2017). Detection of surrogate cells presenting vaccinia virus protein by DNA aptamers. Bull. Korean Chem. Soc. 38, 123-126.

1128. Cortina, C., Turon, G., Stork, D., Hernando-Momblona, X., Sevillano, M., Aguilera, M., Tosi, S., Merlos-Suárez, A., Stephan-Otto Attolini, C., Sancho, E. & Batlle, E.A. (2017). Genome editing approach to study cancer stem cells in human tumors. EMBO Mol Med. 9, 869-879.

1127. Coutinho, P.B.D., Friedl, C., Buchholz, R. & Stute, S.C. (2017). Chemical regulation of Fea1 driven transgene expression in Chlamydomonas reinhardtii. Algal Research-Biomass Biofuels Bioproducts 26, 323-329.

1126. Cruz-Teran, C.A., Tiruthani, K., Mischler, A. & Rao, B.M. (2017). Inefficient ribosomal skipping enables simultaneous secretion and display of proteins in Saccharomyces cerevisiae. ACS Synth Biol. Aug 14. doi: 10.1021/acssynbio.7b00144. [Epub ahead of print]

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1124. Dolan, M.J., Luan, H., Shropshire, W.C., Sutcliffe, B., Cocanougher, B., Scott, R.L., Frechter, S., Zlatic, M., Jefferis, G.S.X.E. & White, B.H. (2017). Facilitating neuron-specific genetic manipulations in Drosophila melanogaster using a split GAL4 repressor. Genetics 206, 775-784.

1123. Dong, B., Hu, H.H., Li, Z.F., Cheng, R.Q., Meng, D.M., Wang, J. & Fan, Z.C. (2017). A novel bicistronic expression system composed of the intraflagellar transport protein gene ift25 and FMDV 2A sequence directs robust nuclear gene expression in Chlamydomonas reinhardtii. Appl. Microbiol. Biotechnol. 2017 Feb 25. doi: 10.1007/s00253-017-8177-9. [Epub ahead of print]

1122. Dong, Y., Ren, Y.C., Yang, M.S., Zhang, J., Qiu, T. & Cui, K.L. (2017). Construction of a new type of multi-gene plant transformation vector and genetic transformation of tobacco. Biologia Plantarum 61, 13-23.

1121. Efimova, V.S., Isaeva, L.V., Makeeva, D.S., Rubtsov, M.A. Novikova, L.A. (2017). Expression of cholesterol hydroxylase/lyase system proteins in yeast S. cerevisiae cells as a self-processing polyprotein. Mol. Biotechnol. 59, 394-406.

1120. Farruggio, A.P., Bhakta, M.S., du Bois, H., Ma, J. P Calos, M. (2017). Genomic integration of the full-length dystrophin coding sequence in Duchenne muscular dystrophy induced pluripotent stem cells. Biotechnol. J. 12(4). doi: 10.1002/biot.201600477. Epub 2017 Mar 15.

1119. Flerin, N.C., Chen, H., Glover, T.D., Lamothe, P.A., Zheng, J.H., Fang, J.W., Ndhlovu, Z.M., Newell, E.W., Davis, M.M., Walker, B.D. & Goldstein, H. (2017). T-Cell receptor (TCR) clonotype-specific differences in inhibitory activity of HIV-1 cytotoxic T-cell clones is not mediated by TCR alone. J. Virol. 91(6). pii: e02412-16. doi: 10.1128/JVI.02412-16. Print 2017 Mar 15.

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1091. Qin, X., Park, B.O., Liu, J., Chen, B., Choesmel-Cadamuro, V,, Belguise, K., Heo, W.D. & Wang, X. (2017). Cell-matrix adhesion and cell-cell adhesion differentially control basal myosin oscillation and Drosophila egg chamber elongation. Nat Commun. Apr 13;8:14708. doi: 10.1038/ncomms14708.

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1089. Röder, J., Fischer, R. & Commandeur, U. (2017). Adoption of the 2A ribosomal skip principle to Tobacco Mosaic Virus for peptide display. Front. Plant Sci. 8:1125. doi: 10.3389/fpls.2017.01125. eCollection 2017.

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1086. Schuetze, T. & Meyer, V. (2017). Polycistronic gene expression in Aspergillus niger. Microb Cell Fact. 16(1):162. doi: 10.1186/s12934-017-0780-z.

1085. Schwarzhans, J-P., Lutterman, T., Geier, M., Kalinowski, J. & Friehs, K. (2017). Towards systems metabolic engineering in Pichia pastoris. Biotech. Adv. https://doi.org/10.1016/j.biotechadv.2017.07.009.

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1083. Serra-Soriano, M., Antonio Navarro, J. & Pallás V. (2017). Dissecting the multifunctional role of the N-terminal domain of the Melon necrotic spot virus coat protein in RNA packaging, viral movement and interference with antiviral plant defence. Mol Plant Pathol. 18, 837-849.

1082. Shemetov, A.A., Oliinyk, O.S. & Verkhusha, V.V. (2017). How to increase brightness of near-infrared fluorescent proteins in mammalian cells. Cell Chem. Biol. 2017 Jun 7. pii: S2451-9456(17)30181-2. doi: 10.1016/j.chembiol.2017.05.018. [Epub ahead of print]

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85. Sun, Q. & Han, H. (2004). Design and efficacy of a gene trap system for secretory proteins. Prog. Biochem. Biophys. 31, 328-333.

84. Szymczak, A.L., Workman, C.J., Wang, Y., Vignali, K.M., Dilioglou, S., Vanin, E.F. & Vignali, D.A. (2004). Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector. Nature Biotech. 22, 589-94.

83. Takeda, A.,  Kaido, M., Okuno, T. & Mise, K. (2004). The C terminus of the movement protein of Brome mosaic virus controls the requirement for coat protein in cell-to-cell movement and plays a role in long-distance movement. J. Gen. Virol. 85, 1751-1761.

82. Tessitore, A., del P Martin, M., Sano, R., Ma, Y., Mann, L., Ingrassia, A., Laywell, E.D., Steindler, D.A., Hendershot, L.M. & d'Azzo, A. (2004). GM1-ganglioside-mediated activation of the unfolded protein response causes neuronal death in a neurodegenerative gangliosidosis. Mol Cell. 15, 753-66.



2003

81. Baranov, P.V., Gurvich, O.L., Hammer, A.W., Gesteland, R.F. & Atkins, J.F. (2003). RECODE 2003. Nucleic Acids Res. 31, 87-89.

80. Binder, J.J., Hoffman, M.A. & Palmenberg, A.C. (2003). Genetic stability of attenuated mengovirus vectors with duplicate primary cleavage sequences. Virology 312, 481-494.

79. de Felipe, P. & Izquierdo, M. (2003). Construction and characterization of pentacistronic retrovirus vectors. J. Gen. Virol.  84, 12811285.

78. de Felipe, P. Hughes, L.E. Ryan, M.D. & Brown, J.D. (2003). Co-translational, intraribosomal cleavage of polypeptides by the foot-and-mouth disease virus 2A peptide. J. Biol. Chem. 278, 11441-11448.

77. Harvey, T.J., Anraku, I., Linedale, R., Harrich, D., Mackenzie, J., Suhrbier, A. & Khromykh, A.A. (2003). Kunjin virus replicon vectors for human immunodeficiency virus vaccine development. J Virol. 77, 7796-7803.

76. Jackson, C.A, Messinger, J., Palmer, M.T., Peduzzi, J.D. & Morrow, C.D. (2003). Gene expression in the muscle and central nervous system following intramuscular inoculation of encapsidated or naked poliovirus replicons. Virology 314, 45-61.

75. Martin, E., Kamath, A.T., Briscoe, H., & Britton, W.J. (2003) The combination of plasmid interleukin-12 with a single DNA vaccine is more effective than Mycobacterium bovis (bacille Calmette-Guerin) in protecting against systemic Mycobacterim avium infection.  Immunology 109, 308-314.

74. Nakaya, Y., Zheng, H. & Garcia-Sastre, A. (2003) Enhanced cellular immune responses to SIV Gag by immunization with influenza and vaccinia virus recombinants. Vaccine 21, 2097-2106.

73. Novak, M.J., Moldoveanu, Z., Huang, W.Q., Jackson, C.A., Palmer, M.T., McPherson, S.A. & Morrow, C.D. (2003) Intramuscular immunization with poliovirus replicons primes for a humoral and cellular immune response to soluble antigen. Viral Immunology 16, 169-182.

72. Schiedlmeier, B., Klump, H., Will, E., Arman-Kalcek, G., Li, Z., Wang, Z., Rimek, A., Friel, J., Baum, C. & Ostertag, W. (2003). High-level ectopic HOXB4 expression confers a profound in vivo competitive growth advantage on human cord blood CD34+ cells, but impairs lymphomyeloid differentiation. Blood 101, 1759-1768.

71. Shi, P-Y. (2003)/ Genetic systems of West Nile virus and their potential applications. Curr. Op. in Investigational Drugs 4, 959-965.

70. Straathof, K.C., Spencer, D.M., Sutton, R.E. & Rooney, C.M. (2003). Suicide genes as safety switches in T lymphocytes. Cytotherapy 5, 227-230.

69. Thomas, J.M., Klimstra, W.B., Ryman, K.D. & Heidner, H.W. (2003) Sindbis virus vectors designed to express a foreign protein as a cleavable component of the viral structural polyprotein. J. Virol. 77, 5598-5606.

68. Ward, S.M., Tindle, R.W., Khromykh, A.A. & Gowans, E.J. Generatino of CTL responses using Kunjin replicon RNA. Immunol. Cell Biol. 81, 73-78.


2002

67. Anraku, I., Harvey, T.J., Linedale, R., Gardner, J., Harrich, D., Suhrbier, A. & Khromykh, A.A. (2002). Kunjin virus replicon vaccine vectors induce protective CD8+ T-cell immunity. J. Virol. 76, 3791-3799.

66. Awram, P., Gardner, R.C., Forster, R.L. & Bellamy, A.R. (2002). The potential of plant viral vectors and transgenic plants for subunit vaccine production. Adv. Virus Res. 58, 81-124.

65. Contag, C.H. & Bachmann, M.H. (2002). Advances in in vivo bioluminescence imaging of gene expression. Annu. Rev. Biomed. Eng. 4, 235-260.

64. de Felipe, P. (2002). Polycistronic viral vectors. Curr Gene Ther. 2, 355-378.

63. François, I.E.J.A., Broekaert, W.F. & Cammue, B.P.A. (2002a). Different approaches for multi-transgene-stacking in plants. Plant Sci. 163, 281-295.

62. François, I.E., De Bolle, M.F., Dwyer, G., Goderis, I.J., Woutors, P.F., Verhaert, P.D., Proost, P., Schaaper, W.M., Cammue, B.P. & Broekaert, W.F. (2002). Transgenic expression in Arabidopsis of a polyprotein construct leading to production of two different antimicrobial proteins. Plant Physiol. 128, 1346-1358.

61. Hope, J.C., Kwong, L.S., Entrican, G., Wattegedera, S., Vordermeier, H.M., Sopp, P. & Howard, C.J. (2002) Development of detection methods for ruminant interleukin (IL)-12.   J. Immunological Methods 266,  117-126.

60. Kinsella, T.M., Ohashi, C.T., Harder, A.G., Yam, G.C., Li, W., Peelle, B., Pali, E.S., Bennett, M.K., Molineaux, S.M., Anderson, A., Masuda, E.S. & Payan, D.G. (2002) Retrovirally delivered random cyclic peptide libraries yield inhibitors of interleukin-4 signalling in human B cells. J. Biol. Chem. 277, 37512-37518.

59. Liu, W.J., Sedlak, P.L., Kondratieva, N. & Khromykh, A.A. (2002). Complementation analysis of the flavivirus Kunjin NS3 and NS5 proteins defines the minimal regions essential for formation of a replication complex and shows a requirement of NS3 in cis for virus assembly. J. Virol. 76, 10766-10755.

58. Ma, C. & Mitra, A. (2002) Expressing multiple genes in  a single open reading frame with the 2A region of foot-and-mouth disease virus as a linker. Molecular Breeding 9, 191-199.

57. Ma, C & Mitra, A. (2002) Instrinsic direct repeats generate consistent post-transcriptional gene silencing in tobacco. The Plant Journal 31, 37-49.

56. Mlotshwa, S., Verver, J., Sithole-Niang, I., Gopinath, K., Carette, J., van Kammen, A. & Wellink J. (2002). Subcellular location of the helper component-proteinase of cowpea aphid-borne mosaic virus. Virus Genes 25, 207-216.

55. Palendira, U., Kamath, A.T., Feng, C.G., Martin, E., Chaplin, P.J., Triccas, J.A. & Britton, W.J. (2002). Coexpression of interleukin-12 chains by a self-splicing vector increases the protective cellular immune response of DNA and Mycobacterium bovis BCG vaccines against Mycobacterium tuberculosis. Infection and Immunity 70,  1949-1956.

54. Pouwels, J., Carette, J.E., van Lent, J. & Wellink, J. (2002). Cowpea mosiac virus: effects on host cell processes. Mol. Plant. Pathol. 3, 411-418.

53. Rayner, J.O., Dryga, S.A. & Kamrud, K.I. (2002). Alphavirus vectors and vaccination. Rev. Med. Virol. 12, 279-296.

52. Ryan, M.D., Luke, G., Hughes, L.E., Cowton, V.M., ten Dam, E., Li, X., Donnelly, M.L.L., Mehrotra, A. & Gani, D. (2002). The aphtho- and cardiovirus ‘primary’ 2A/2B polyprotein ‘cleavage’. In ‘Molecular Biology of Picornaviruses’ Semler, B. and Wimmer, E. (eds.) pp. 213-223. ASM Press, Washington, USA.

51. Takasuka, N., Enami, M., Itamura, S. & Takemori, T. (2002). Intranasal inoculation of a recombinant influenza virus containing exogenous nucleotides in the NS segment induces  mucosal immune response against the exogenous gene product in mice. Vaccine 20, 1579-1585.

50. Triccas, J.A., Sun, L., Palendira, U. & Britton, W.J. (2002) Comparative affects of plasmid-encoded interleukin 12 and interleukin 18 on the protective efficacy of DNA vaccination against Mycobacterium tuberculosis.  Immunol. Cell Biol. 80  346-350.

49. Yi, M.K., Bodola, F. & Lemon, S.M. (2002) Subgenomic hepatitis C virus replicons inducing expression of a secreted enzymatic reporter protein. Virology 304, 197-210.


2001

48. Ansardi, D.C., Porter, D.C., Jackson, C.A., Gillespie, G.Y. & Morrow, C.D. (2001). RNA replicons derived from poliovirus are directly oncolytic for human tumor cells of diverse origins. Cancer Res. 61, 8470-8479.

47. Essenberg, M. (2001). Prospects for strengthening plant defenses through phytoalexin engineering. Physiol. Mol. Plant Pathol. 59, 71-81.

46. Furler, S., Paterna, J.C., Weibel, M., & Bueler H. (2001). Recombinant AAV vectors containing the foot and mouth disease virus 2A sequence confer efficient bicistronic gene expression in cultured cells and rat substantia nigra neurons. Gene Therapy 8, 864-873.

45. Halpin, C., Barakate, A., Askari, B., Abbott, J. & Ryan, M.D. (2001). Enabling technologies for manipulating multiple genes on complex pathways. Plant Mol. Biol.  47, 295-310.

44. Hunt, A.G. & Maiti, I.B. (2001). Strategies for expressing multiple foreign genes in plants as polycistronic constructs. In Vitro Cell. Dev. Biol. 37, 313-320.

43. Jackson, C. A., Cobbs, C., Peduzzi, J.D., Novak, M. & Morrow, C. D. (2001). Repetitive intrathecal injections of poliovirus replicons result in gene expression in neurons of the Central Nervous System without pathogenesis. Hum. Gene Ther. 12, 1,827-1,841.

42. Klump, H., Schiedlmeier, B., Vogt, B., Ryan, M.D, Ostertag, W. & Baum, C. (2001). Retroviral vector-mediated expression of HoxB4 in hematopoietic cells using a novel coexpression strategy. Gene Therapy  8, 811-817.

41. Luke, G. & Ryan M.D. (2001). Translating the message. Biologist 48, 79-82.

40. Pang, X., Zhang, M. &  Dayton, A.I  (2001). Development of dengue virus replicons expressing HIV-1 gp120 and other heterologous genes: a potential future tool for dual vaccination against dengue virus and HIV. BMC Microbiol. 1 1-28.

39. Sanchez-Navarro, J., Miglino, R., Ragozzino, A. & Bol, J.F. (2001). Engineering of Alfalfa mosaic virus RNA 3 into an expression vector. Arch. Virol. 146, 923-939.


2000

38. Bramel-Verheije, M.H.G., Rottier, P.J.M., & Meulenberg, J.J.M. (2000). Expression of a foreign epitope by porcine reproductive and respiratory syndrome virus. Virology  278, 380-389.

37. de Felipe, P. & Izquierdo, M. (2000). Tricistronic and tetracistronic retroviral vectors for gene transfer. Human Gene Therapy  11, 1921-1931.

36. de Rose, R., Scheerlinck, J.P.Y., Casey, G,, Wood, P.R., Tennent, J,M,, & Chaplin, P.J. (2000). Ovine interleukin-12: Analysis of biologic function and species comparison.   J. Interferon & Cytokine Res. 20, 557-564.

35. García-Sastre, A. (2000). Transfectant influenza viruses as antigen delivery vectors. Adv. Virus Res. 55, 579-597.

34. Gopinath, K., Wellink, J., Porta, C., Taylor, K.M., Lomonossoff, G.P., & van Kammen, A. (2000). Engineering cowpea mosaic virus RNA-2 into a vector to express heterologous proteins in plants. Virology 267, 159-173.

33. Hewson, R. RNA viruses: emerging vectors for vaccination and gene therapy. Mol. Med. Today 6, 28-35.

32. Khromykh, A.A. (2000). Replicon-based vectors of positive strand RNA viruses. Curr. Opin. Mol. Therapeutics 2, 555-569.

31. O'Brien, G.J., Bryant, C.J., Voogd, C., Greenberg, H.B., Gardner, R.C, & Bellamy, A.R. (2000). Rotavirus VP6 expressed by PVX vectors in Nicotiana benthamiana coats PVX rods and also assembles into virus like particles. Virology 270, 444-453.

30. Suzuki, N., Geletka, L.M., & Nuss, D.L. (2000). Essential and dispensable virus-encoded replication elements revealed by efforts to develop hypoviruses as gene expression vectors. J. Virol. 74, 7568-7577.

29. Thomas, C.L. & Maule, A.J. (2000). Limitations on the use of fused green fluorescent protein to investigate structure-function relationships for the cauliflower mosaic virus movement protein. J. Gen. Virol. 81, 1851-1855.

28. Varnavski, A.N., Young, P.R. & Khromykh, A.A. (2000). Stable high-level expression of heterologous genes in vitro and in vivo by noncytopathic DNA-based Kunjin virus replicon vectors.  J. Virol. 74, 4394-4403.

27. Wang, T.H., Rijnbrand, R.C.A. & Lemon, S.M. (2000). Core protein-coding sequence, but not core protein, modulates the efficiency of cap-independent translation directed by the internal ribosome entry site of hepatitis C virus. J. Virol. 74, 11,347-11,358.



1999

26. Chaplin, P.J., Camon, E.B., Villarreal-Ramos, B., Flint, M., Ryan, M.D. & Collins, R.A. (1999). Production of interlukin-12 as a self-processing polypeptide. J. Interferon & Cytokine Res. 19, 235-241.

25. de Felipe, P., Martín, V., Cortés, M.L., Ryan, M.D. & Izquierdo, M. (1999). Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy. Gene Therapy 6, 198-208.

24. Halpin, C., Cooke, S.E., Barakate, A., El Amrani, A. & Ryan, M.D. (1999). Self-processing polyproteins - a system for co-ordinate expression of multiple proteins in transgenic plants. Plant J. 17, 453-459.

23. Kokuho, T., Watanabe, S., Yokomizo, Y. & Inumaru, S. (1999). Production of biologically active, heterodimeric porcine interleukin-12 using a monocistronic baculoviral expression system.  Vet. Immunol.  Immunopathol. 72, 289-302.

22. Smerdou, C. & Liljestrom, P. (1999). Two-helper RNA system for production of recombinant Semliki Forest Virus particles. J. Virol. 73, 1092-1098.

21. Turpen, T.H. (1999). Tobaco mosaic virus and the virescence of biotechnology. Phil. Trans. R. Soc. Lond. B 354, 665-673.

20. Varnavski, A.N. & Khromykh. A,A. (1999). Noncytopathic flavivirus replicon RNA-based system for expression and delivery of heterologous genes. Virology 255, 366-375.



1998

19. Bock, M., Heinkelein, M., Lindemann, D. & Rethwilm, A. (1998). Cells expressing the human foamy virus (HFV) accessory Bet protein are resistant to productive HFV superinfection. Virology 250, 194-204.

18.Collins, R.A., Camon, E.B., Chaplin, P.J. & Howard, C.J. (1998). Influence of IL-12 on interferon-gamma production by bovine leucocyte subsets in response to bovine respiratory syncytial virus. Vet. Imunol. Immunopath. 63, 69-72.

17. Halpin, C. & Ryan, M.D. (1998). Expression of self processing polyproteins in transgenic plants. U.S. Patent 5,846,767. Assignee - Zeneca Limited, London (PCT No. PCT/ GB94/ 02765).

16. Porta, C. & Lomonossoff, G.P. (1998). Scope for using plant viruses to present epitopes from animal pathogens. Rev. Med. Virol. 8, 25-41.

15. Scheerlinck, J.-P. Y., Chaplin, P.J. & Wood, P.R. (1998). Ovine cytokines and their role in the immune response. Vet. Res. 29, 369-383.

14. Smolenska, L., Roberts, L.M., Learmonth, D., Porter, A.J., Harris, W.J., Wilson, T.M.A., & Cruz, S. S. (1998). Production of a functional single chain antibody attached to the surface of a plant virus. Febs Letters 441, 379-382.

13. van der Ryst, E., Nakasone, T., Habel, A., Venet, A.,  Gomard, E., Altmeyer,R., Girard, M, & Borman, A.M. (1998). Study of the immunogenicity of different recombinant Mengo viruses expressing HIV1 and SIV epitopes. Res. in Virol. 149, 5-20.



1997

12. Camon, E.B., Chaplin. P.J., Howard, C.J. & Collins, R.A. (1997).  Modulation of bovine T cell responses by IL-12 and the influence of IL-4 on interferon-gamma responses to respiratory syncytial virus. Biochem. Soc. Trans. 25, S291.

11. Jonson, J., Lin, T. & Lomonossoff, G. (1997). Presentation of heterologous peptides on plant viruses: genetics, structure, and function. Ann. Rev. Phytopathol. 35, 67-86.

10. Nestler, U., Heinkelein, M., Lucke, M., Meixensberger, J., Scheurlen, W., Kretschmer, A. & Rethwilm, A. (1987). Foamy virus vectors for suicide gene therapy. Gene Therapy 4, 1270-1277.

9. Roberts, A.G., Santa Cruz, S., Roberts, I M., Prior, D.A.M., Turgeon, R. and Oparka, K.J. (1997). Phloem unloading in sink leaves of Nicotiana benthamiana: Comparison of a fluorescent solute with a fluorescent virus. The Plant Cell 9, 1381-1396.



1996

8. Cruz, S.S., Chapman, S., Roberts, A.G., Roberts, I.M., Prior, D.A.M., & Oparka,K.J. (1996). Assembly and movement of a plant-virus carrying a green fluorescent protein overcoat. Proc. Natl. Acad. Sci. U.S.A. 93, 6286-6290.

7. Mattion, N.M., Harnish, E.C., Crowley, J.C. & Reilly, P.A. (1996). Foot-and-Mouth Disease Virus 2A protease mediates cleavage in attenuated Sabin 3 poliovirus vectors engineered for delivery of foreign antigens. J. Virol. 70, 8124-8127.



1995

6. García-Sastre, A. & Palese, P. (1995). Influenza virus vectors. Biologicals 23, 171-178.

5. Oparka, K.J., Roberts, A.G., Prior, D.A.M., Chapman, S., Baulcombe, D. & Santa Cruz, S. (1995). Imaging the green fluorescent protein in plants - viruses carry the torch. Protoplasma 189, 133-141.

4. Precious, B., Young, D.F., Bermingham, A., Fearns, R., Ryan, M.D. & Randall, R.E. (1995). Inducible expression of the P, V, and NP genes of the paramyxovirus simian virus 5 in cell lines and an examination of the NP-P and NP-V interactions. J. Virol. 69, 8001-8010.

3. Schmidt, M. & Rethwilm, A. (1995). Replicating Foamy Virus-based vectors directing high-level expression of foreign genes. Virology 210, 167-178.



1994

2. Percy, N., Barclay, W.S., Garcia-Sastre, A. & Palese, P. (1994). Expression of a foreign protein by influenza A virus. J. Virol. 68, 44486-4492.

1. Ryan, M.D. & Drew, J. (1994). Foot-and-mouth disease virus 2A oligopeptide mediated cleavage of an artificial polyprotein. EMBO J. 134, 928-933.


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