Click on the construct maps shown below to access images of the fluorescence patterns

Plasmid	Proteins Comprising the Single, Long, ORF
pPDF20
pPDF29
pPDF46
pPDF68
pPDF19
pPDF97	(darker pink region indicates the signal-anchor sequence of the HN protein)
pPDF18
pPDF17
pPDF96	(darker pink region indicates the signal-anchor sequence of the HN protein)
pPDF61
pPD58
pPDF95
pPDF59
pPDF21
pPDF25
pPDF42
pPDF56
pPDF63
pPDF67
pPDF85
pPDF93

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Abbreviations

(i) Genes: EYFP - enhanced yellow fluorescent protein; ECFP - enhanced cyan fluorescent protein; HN - heamagluttinin/neuraminidase gene from virus SV5 (type II signal-anchor sequence); pac - puromycin resistance

2A - self-cleaving 23aa sequence, 2Amut - inactive, point mutant of 2A.

(ii) Targeting Signals: MT - mitochondrial targeting sequence; NC - nuclear localisation signal; GT - GalT Golgi targeting (type II signal-anchor sequence); CR - calreticulin endoplasmic reticulum (ER) targeting type I (cleaved) signal sequence.

The constucts shown below generate multiple proteins from a single gene.

Cells are transfected with a single plasmid.

Cell-lines expressing all of the proteins can be rapidly and efficiently established by puromycin selection - resistance being conferred by the pac gene.

Funded by the Wellcome Trust (Grant ref. 064814/Z/01/Z)