2A in Biomedicine & Biotechnology

Research papers using 2A, plus reviews discussing the use of 2A

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Comment upon Sommer et al., (2009). iPS cell generation using a single lentiviral stem cell cassette. Stem Cells 27, 543-549.

'Converting Adult Somatic Cells to Pluripotent Stem Cells using a Single Virus' - Physorg.com

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Comment upon Holst et al., (2006). Rapid analysis of T-cell selection in vivo using T cell-receptor retrogenic mice. Nature Methods 3, 191-197.

'Retroviral TCR Gene Transduction: 2A for Two'. Bosselut, R. (2006). News & Views, Nature Methods 3, 162-164.

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Comment upon Fang et al., (2005). Stable antibody expression at therapeutic levels using the 2A peptide. Nature Biotechnol. 23, 584-590.

'Therapeutic Antibody Gene Transfer' - 'News and Views' discussion the technology for gene therapy in Nature Biotechnology.

'Viral 2A Peptide Stabilizes Monoclonal Antibody at Therapeutic Levels' Gene Therapy Weekly

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Comment upon Szymczak et al., (2004). Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector. Nature Biotechnol. 22, 589-94.

'Put all your Genes in One Basket ' - a highlight of the technology for gene therapy in Nature Methods

'Caught in Translation ' - a highlight of the technology for gene therapy in Nature Immunology

'Multiple Gene products from a single vector; 'self-cleaving 2A peptides' Gene Therapy 11, 1673-1674 (2004).

'Virus Trick Could Turn Cells into Drug Factories' Better Humans

'Genetic Trick Adapted From Viruses Makes Gene Therapy Vectors More Versatile'

Science Daily ..Innovations Report...Charity Wire...St. Judes Childrens Research Hospital.. News-Medical.Net.... Techmonitor.net..bio.com

'Gene Therapy Enhanced by Virus Peptides' .....MedMarkets

 

A new function (translational recoding plus co-translational signal sequence) of cellular "2A-like" sequences that produces dual protein targeting...

Roulston, C., Luke, G.A., de Felipe, P., Ruan, L., Cope, J., Nicholson, J., Sukhodub, A., Tilsner, J. & Ryan, M.D. (2016). ‘2A-Like’ signal sequences mediating translational recoding: A novel form of dual protein targeting. Traffic 17, 923-939.

2023

1558. Gristick, H.B., Hartweger, H., Loewe, M., van Schooten, J., Ramos, V., Oliveira, T.Y., Nishimura, Y., Koranda, N.S., Wall, A., Yao, K.H., Pos8on, D., Gazumyan, A., Wiatr, M., Horning, M., Keeffe, J.R., Hoffmann, M.A.G., Yang, Z., Abernathy, M.E., Dam, K.A., Gao, H., Gnanapragasam, P.N.P., Kakutani, L.M., Pavlovitch-Bedzyk, A.J., Seaman, M.S., Howarth, M., McGuire, A.T., Stamatatos, L., Martin, M.A., West, A.P. Jr, Nussenzweig, M.C. & Bjorkman, P.J. (2023). CD4 binding site immunogens elicit heterologous anti-HIV-1 neutralizing antibodies in transgenic and wild-type animals. Sci Immunol. 2023 Feb 17;8(80):eade6364. doi: 10.1126/sciimmunol.ade6364. Epub 2023 Feb 10. PMID: 36763635.

1557. Hoang, H.D., Said, A., Vaidya, N., Gilchrist, V.H., Malone, K., Kabilan, U., Topshee, S., Xiang, X., Yang, A.D., Olagnier, D., Mossman, K., Beug, S.T., Jafarnejad, S.M., Workenhe, S.T., Graber, T.E. & Alain, T. (2023). Adaptation of transgene mRNA translation boosts the anticancer efficacy of oncolytic HSV1. J Immunother Cancer. 2023 Mar;11(3):e006408. doi: 10.1136/jitc-2022-006408. PMID: 36958764.

1556. Kemmler, C.L., Moran, H.R., Murray, B.F., Scoresby, A., Klem, J.R., Eckert, R.L., Lepovsky, E., Bertho, S., Nieuwenhuize, S., Burger, S., D'Agati, G., Betz, C., Puller, A.C., Felker, A., Ditrychova, K., Bötschi, S., Affolter, M., Rohner, N., Lovely, C.B., Kwan, K.M., Burger, A. & Mosimann C. (2023). Next-generation plasmids for transgenesis in zebrafish and beyond. Development 150(8):dev201531. doi: 10.1242/dev.201531. Epub 2023 Apr 19. PMID: 36975217.

1555. Kirk, N.M., Huang, Q., Vrba, S., Rahman, M., Block, A.M., Murphy, H., White, D.W., Namugenyi, S.B., Ly, H., Tischler, A.D. & Liang, Y. (2023). Recombinant Pichinde viral vector expressing tuberculosis antigens elicits strong T cell responses and protection in mice. Front. Immunol. 14:1127515. doi: 10.3389/fimmu.2023.1127515.

1554. Meurant, S., Mauclet, L., Dieu, M., Arnould, T., Eyckerman, S. & Renard, P. (2023). Endogenous TOM20 proximity labeling: a Swissknife
for the study of mitochondrial proteins in human cells. Preprint doi:10.20944/preprints202212.0304.v2.

1553. Ordon, J., Kiel, N., Becker, D., Kretschmer, C., Schulze-Lefert, P. & Stuttmann, J. (2023). Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two. Plant Methods. 2023 Mar 28;19(1):30. doi: 10.1186/s13007-023-01010-4. PMID: 36978193; PMCID: PMC10053088.

1552. Sherlock, M.E., Baquero Galvis, L., Vicens, Q., Kieft, J.S. & Jagannathan, S. (2023). Principles, mechanisms, and biological implications of translation termination-reinitiation. RNA. 2023 Apr 6:rna.079375.122. doi: 10.1261/rna.079375.122. Epub ahead of print. PMID: 37024263..

1551. Teng, X., Dai, Y., Li, K., Wu, Y., Hou, H. & Li, J. (2023). LiveG4ID-Seq for profiling the dynamic landscape of chromatin G-quadruplexes during cell cycle in living cells. Small Methods. 2023 Feb 5:e2201487. doi: 10.1002/smtd.202201487. Epub ahead of print. PMID: 36739600.

1550. Wang, M., Shang, Liu, X. & Chen, S. (2023). Assembly of nitrogenase biosynthetic pathway in Saccharomyces cerevisiae by using polyprotein strategy. Front. Microbiol. 14:1137355. doi: 10.3389/fmicb.2023.1137355.

1549. Zhu, X., Ricci-Tam, C., Hager, E.R. & Sgro, A.E. (2023) Self-cleaving peptides for expression of multiple genes in Dictyostelium discoideum. PLoS ONE 18(3): e0281211.

2022

1548. Bindics, J., Khan, M., Uhse, S., Kogelmann, B., Baggely, L., Reumann, D., Ingole, K.D., Stirnberg, A., Rybecky, A., Darino, M., Navarrete, F., Doehlemann, G. & Djamei, A. (2022). Many ways to TOPLESS - manipulation of plant auxin signalling by a cluster of fungal effectors. New Phytol. doi: 10.1111/nph.18315. Online ahead of print.

1547. Carpenter, A., Santos, S.R. & Clem, R.J. (2022). Expressing the pro-apoptotic Reaper protein via insertion into the structural open reading frame of Sindbis Virus reduces the ability to infect Aedes aegypti Mosquitoes. Viruses 14, 2035.

1546. Cave, D.D., Buonaiuto, S., Sainz, B. Jr., Fantuz, M., Mangini, M., Carrer, A., Di Domenico, A., Iavazzo, T.T., Andolfi, G., Cortina, C., Sevillano, M., Heeschen, C., Colonna, V., Corona, M., Cucciardi, A., Di Guida, M., Batlle, E., De Luca, A. & Lonardo, E. (2022). LAMC2 marks a tumor-initiating cell population with an aggressive signature in pancreatic cancer. J. Exp. Clin. Cancer Res. 41(1):315.

1545. Cline, H.T. (2022). Imaging structural and functional dynamics in Xenopus neurons. Cold Spring Harb Protoc; doi:10.1101/pdb.top106773.

1544. Duan, X.Y., Tian, Y., Song, Z.Q., Song, L.P., Lin, W.B., Wang, C., Yang, H., Lu, X.Y., Ji, X.J. & Liu, H.H. (2022). High-level de novo biosynthesis of cordycepin by systems metabolic engineering in Yarrowia lipolytica. Bioresour. Technol. 127862. doi: 10.1016/j.biortech.2022.127862. Online ahead of print.

1543. Falcon, C., Smith, L., Al-Obaidi, M., Abu Zaanona, M., Purvis, K., Minagawa, K., Athar, M., Salzman, D., Bhatia, R., Goldman, F. & Di Stasi, A. (2022). Combinatorial suicide gene strategies for the safety of cell therapies. Front. Immunol. 13:975233.

1542. Fang, E., Liu, X., Li, M., Liu, J., Zhang, Z., Liu, X., Li, X., Li, W., Peng, Q., Yu, Y. & Li, Y. (2022). Construction of a Dengue nanoLuc reporter virus for in vivo live imaging in mice. Viruses 14(6):1253.

1541. Hashemi, L., Ormsbee, M.E., Patel, P.J., Nielson, J.A., Ahlander, J. & Padash Barmchi, M. (2022). A Drosophila model of HPV16-induced cancer reveals conserved disease mechanism. PLoS One. 2022 Dec 12;17(12):e0278058. doi: 10.1371/journal.pone.0278058. PMID: 36508448; PMCID: PMC9744332.

1540. Iggo R. (2022) Lentiviral transduction of mammary epithelial cells. In: Mammary Stem Cells, Methods in Molecular Biology. Vivanco M. (ed) vol 2471. Humana, New York, N.Y.

1539. Ishida, K,, Tatsumi, K., Minamigawa, Y., Mori, K., Matsumaru, D., Nagase, H., Kanda, Y., Takuma, K. & Nakanishi, T. (2022). Neuronal differentiation reporter mice as a new methodology for detecting in vivo developmental neurotoxicity. Biochem. Pharmacol. 206:115332.

1538. Jacobsen, R.I., Nair, R.R., Obenhaus, H.A., Donato, F., Slettmoen, T., Moser, M.B. & Moser, E.I. (2022). All-viral tracing of monosynaptic inputs to single birthdate-defined neurons in the intact brain. Cell Rep. Methods 2(5):100221.

1537. Javaid, N. & Choi, S. (2022). An alternative approach to generate induced pluripotent stem cells with precise CRISPR/Cas9 tool. Stem Cells Int. Article ID 4537335.

1536 Joest, E.F., Winter, C., Wesalo, J.S., Deiters, A. & Tampé, R. (2022). Efficient amber suppression via ribosomal skipping for in situ synthesis of photoconditional nanobodies. ACS Synth Biol. doi: 10.1021/acssynbio.1c00471. Online ahead of print.

1535. Kauth, L., Buhl, E.M., Luka, J., Richter, K., Commandeur, U. & Dickmeis, C. (2022). Plug-and-display photo-switchable systems on plant virus nanoparticles. BioTech (Basel). 11(4):49.

1534. Lajevardi, M.S., Taheri, T., Gholami, E., Seyed, N. & Rafati, S. (2022). Structural analysis of PpSP15 and PsSP9 sand fly salivary proteins designed with a self-cleavable linker as a live vaccine candidate against cutaneous leishmaniasis. Parasit Vectors Oct 19;15(1):377.

1533. Li, H., Tang, Y., Ren, J., Bai, R., Hu, L., Jia, W., Cao, Y., Hong, L., Xu, M., Gao, S., Shi, Y., Pan, S., Wang, L., Zheng, K., Zhao, S. & Wang, H. (2022). Identification of novel B-1 transitional progenitors by B-1 lymphocyte fate-mapping transgenic mouse model Bhlhe41dTomato-Cre. Front. Immunol. 13:946202.

1532. Liu, X., Zhang, P., Zhao, Q. & Huang, A.C. (2022). Making small molecules in plants: A chassis for synthetic biology-based production of plant natural products. J. Integr. Plant. Biol. 2022 Jul 19. doi: 10.1111/jipb.13330. Epub ahead of print.

1531. Moradian, H., Gossen, M. & Lendlein, A. (2022). Co-delivery of genes can be confounded by bicistronic vector design. MRS Comm. https://doi.org/10.1557/s43579-021-00128-7.

1530. Noor, S.M. & Norazit, A. (2022). Fishing for synucleinopathy models. Fisheries Aquatic. Sci. 25, 117-139

1529. Ong, H.K., Nguyen, N.T.B., Bi, J. & Yang, Y. (2022). Vector design for enhancing expression level and assembly of knob-into-hole based FabscFv-Fc bispecific antibodies in CHO cells. Antibody Therapeutics, 2022 1–13.

1528. Pham, H.T.A., Lee, S. & Lee, Y.J. (2022). Bicistronic reporter mice for monitoring of Fgf21 expression. Biochem Biophys Res Commun. 619, 104-109. Online ahead of print.

1527. Philip, A.A. & Patton, J.T. (2022). Generation of recombinant rotaviruses expressing human norovirus capsid proteins. J. Virol. :e0126222.

1526. Podschwadt, P., Malyshkina, A., Windmann, S., Werner, T., Hansen, W. & Bayer, W. (2022). A detailed analysis of F-MuLV- and SFFV-infected cells in Friend virus-infected mice reveals the contribution of both F-MuLV- and SFFV-infected cells to the interleukin-10 host response. Retrovirology. 2022 Dec 16;19(1):29. doi: 10.1186/s12977-022-00613-4. PMID: 36527061; PMCID: PMC9758943.

1525. Sadasivan, J., Vlok, M., Wang, X., Nayak, A., Andino, R. & Jan, E. (2022). Targeting Nup358/RanBP2 by a viral protein disrupts stress granule formation. PLoS Pathog. 2022 Dec 1;18(12):e1010598. doi: 10.1371/journal.ppat.1010598. PMID: 36455064; PMCID: PMC9746944.

1524. Schenstnyi, K., Strauß, A., Dressel, A., Morbitzer, R., Wunderlich, M., Andrade, A.G., Phan, T.T., Aguilera, P.L.A., Brancato, C., Berendzen, K.W. & Lahaye, T. (2022) .The tomato resistance gene Bs4 suppresses leaf watersoaking phenotypes induced by AvrHah1, a transcription activator-like effector from tomato-pathogenic xanthomonads. New Phytol. Sep 2. doi: 10.1111/nph.18456. Online ahead of print.

1523. Singh, R., Chandel, S., Ghosh, A., Gautam, A., Huson, D.H., Ravichandiran, V. & Ghosh, D. (2022). Easy efficient HDR-based targeted knock-in in Saccharomyces cerevisiae genome using CRISPR-Cas9 system. Bioengineered. 2022 Jun;13(6):14857-14871. doi: 10.1080/21655979.2022.2162667. PMID: 36602175..

1522. Smith, L. & Di Stasi, A. (2022). Safety switches used for cellular therapies. In Gene and Cellular Immunotherapy for Cancer, Cancer Drug Discovery and Development pp 91-107. Ghobadi, A. & DiPersio, J.F. (eds.). Springer Nature, Switzerland https://doi.org/10.1007/978-3-030-87849-8_6.

1521. Stern, L.A., Gholamin, S., Moraga, I., Yang, X., Saravanakumar, S., Cohen, J.R., Starr, R., Aguilar, B., Salvary, V., Hibbard, J.C., Kalbasi, A., Shepphird, J.K., O'Hearn, J., Garcia, K.C. & Brown, C.E. (2022). Engineered IL13 variants direct specificity of IL13Ra2-targeted CAR T cell therapy. Proc Natl Acad Sci USA. 119(33):e2112006119.

1520. Strubel, A., Münick, P., Chaikuad, A., Dreier, B., Schaefer, J., Gebel, J., Osterburg, C., Tuppi, M., Schäfer, B., Knapp, S., Plückthun, A. & Dötsch, V. (2022). Designed ankyrin repeat proteins as a tool box for analyzing p63. Cell Death Differ. doi: 10.1038/s41418-022-01030-y. Online ahead of print.

1519. Tavares-Esashika, M.L., Campos, R.N.S., Maeda, M.H.K., Koyama, L.H.H., Hamann, P.R.V., Noronha, E.F. & Nagata, T. (2022). Development of a heterologous gene expression vector in plants based on an infectious clone of a tobravirus, pepper ringspot virus. Annals of App. Biol. 1–10.

1518. Tsai, S.J., Ai, Y., Guo, C. & Gould, S.J. (2022). Degron-tagging of BleoR and other antibioticresistance genes selects for higher expression of linked transgenes and improved exosome engineering, J. Biol. Chem. (in the press). doi: https://doi.org/10.1016/j.jbc.2022.101846.

1517. Ueda, H.H., Nagasawa, Y., Sato, A., Onda, M. & Murakoshi, H. (2022). Chronic neuronal excitation leads to dual metaplasticity in the signaling for structural long-term potentiation. Cell Rep. 38(1):110153

1516. Wang, Z., McWilliams-Koeppen, H.P., Reza, H., Ostberg, J.R., Chen, W., Wang, X., Huynh, C., Vyas, V., Chang, W.C., Starr, R., Wagner, J.R., Aguilar, B., Yang, X., Wu, X., Wang, J., Chen, W., Koelker-Wolfe, E., Seet, C.S., Montel-Hagen, A., Crooks, G.M., Forman, S.J. & Brown, C.E. (2022). 3D-organoid culture supports differentiation of human CAR+ iPSCs into highly functional CAR T cells. Cell Stem Cell. S1934-5909(22)00060-1. doi: 10.1016/j.stem.2022.02.009. Online ahead of print.

1515. Zhang, J.H., Shan, L-L., Liang, F., Du, C-Y. & Li, J-J. (2022). Strategies and considerations for improving recombinant antibody production and quality in Chinese Hamster Ovary cells. Front. Bioeng. Biotech. 10, Article 856049.

1514. Zhang, Y., Ni, L., Chen, S., Qin, Y., Ding, X., Li, J., Pan, Y. & Zhang, X. (2022). Pterostilbene production of tomato transformed with resveratrol synthase and resveratrol O-methyltransferase genes. Plant Sci. 322:111343. doi: 10.1016/j.plantsci.2022.111343. Online ahead of print.

1513. Zhu, X, Ricci-Tam, C. & Sgro, A.E. (2022). Self-cleaving 2A peptides allow for expression of multiple genes in Dictyostelium discoideum. BioRxiv preprint doi: https://doi.org/10.1101/2022.03.08.482734.

2021

1512. Ashley, G.E., Duong, T., Levenson, M.T., Martinez, M.A.Q., Johnson, L.C., Hibshman, J.D., Saeger, H.N., Palmisano, N.J., Doonan, R., Martinez-Mendez, R., Davidson, B.R., Zhang, W., Ragle, J.M., Medwig-Kinney, T.N., Sirota, S.S., Goldstein, B., Matus, D.Q., Dickinson, D.J., Reiner, D.J. & Ward, J.D., (2021). An expanded auxin-inducible degron toolkit for Caenorhabditis elegans. Genetics 217, iyab006.

1511. Bacon, K., Burroughs, M., Blain, A., Menegatti, S. & Rao, B.M. (2021). Screening yeast display libraries against magnetized yeast cell targets enables efficient isolation of membrane protein binders. ACS Comb. Sci. 21, 817-832.

1510. Bahar, M.W., Porta, C., Fox, H., Macadam, A.J., Fry, E.E. & Stuart, D.I. (2021). Mammalian expression of virus-like particles as a proof of principle for next generation polio vaccines. NPJ Vaccines 6(1):5.

1509. Becker, I., Prasad, B., Ntefidou, M., Daiker, V., Richter, P. & Lebert, M. (2021). Agrobacterium tumefaciens-mediated nuclear transformation of a biotechnologically important microalga — Euglena gracilis. Int. J. Mol. Sci. 22, 6299.

1508. Bieri, M., Hendrickx, R., Bauer, M., Yu, B., Jetzer, T., Dreier, B., Mittl, P.R.E., Sobek, J., Plückthun, A., Greber, U.F. & Hemmi, S. (2021). The RGD-binding integrins avß6 and avß8 are receptors for mouse adenovirus-1 and -3 infection. PLoS Pathog. 2021 Dec 15;17(12):e1010083.

1507. Cha, S.E., Kujawski, M.J., Yazaki, P., Brown, C. & Shively, J.E. (2021). Tumor regression and immunity in combination therapy with anti-CEA chimeric antigen receptor T cells and anti-CEA-IL2 immunocytokine. Oncoimmunology 10(1):1899469.

1506. Chandler, R.J., Venturoni, L.E., Liao, J., Hubbard, B.T., Schneller, J.L., Hoffmann, V., Gordo, S., Zang, S.W., Ko, C.W. & Chau, N. (2021). Promoterless, nuclease-free genome editing confers a growth advantage for corrected hepatocytes in mice With methylmalonic acidemia. Hepatology 73, 2223-2237.

1505. Choi, E.H., Suh, S., Einstein, D.E, Leinonen, H., Dong, Z., Rao, S.R., Fliesler, S.J., Blackshaw, S., Yu, M., Peachey, N.S, Palczewski, K. & Kiser, P.D. (2021). An inducible Cre mouse for studying roles of the RPE in retinal physiology and disease. JCI Insight Mar 30;146604.

1504. Chowdhury, E.A., Meno-Tetang, G., Chang, H.Y., Wu, S., Huang, H.W., Jamier, T., Chandran, J. & Shah, D.K. (2021). Current progress and limitations of AAV mediated delivery of protein therapeutic genes and the importance of developing quantitative pharmacokinetic/ pharmacodynamic (PK/PD) models. Adv. Drug Deliv. Rev. 170, 214-237.

1503. Dahlin, L.R & Guarneri, M.T. (2021). Development of the high-productivity marine microalga, Picochlorum renovo, as a photosynthetic protein secretion platform. Algal Res. 54102197.

1502. Dahlke, J., Schott, J.W., Vollmer Barbosa, P., Klatt, D., Selich, A., Lachmann, N. , Morgan, M., Moritz, T. & Schambach, A. (2021). Efficient genetic safety switches for future application of iPSC-derived cell transplants. J. Pers. Med. 11, 565.

1501. Das, S., Alam, M.M., Zhang, R., Hisano, S. & Suzuki, N. (2021). Proof of concept of the Yadokari nature: a capsidless replicase-encoding but replication-dependent positive-sense single-stranded RNA virus hosted by an unrelated double-stranded RNA virus. J. Virol. 95:e0046721.

1500. Dierick, H., Ben-Shahar, Y., Raman, B. & Gabbiani, F. (2021). Genetic and viral approaches to record or manipulate neurons in insects.
Curr Opin Insect Sci. S2214-5745(21)00110-3.

1499. Efimova, V.S., Isaeva, L.V., Orekhov, P.S., Bozdaganyan, M.E., Rubtsov, M.A. & Novikova, L.A. (2021). Using a viral 2A peptide-based strategy to reconstruct the bovine P450scc steroidogenic system in S. cerevisiae Bovine P450scc system expression using 2A peptides. J. Biotechnol. 325, 186-195.

1498. Fang, L., Wei, X.Y., Liu, L.Z., Zhou, L.X., Tian, Y.P., Geng, C. & Li, X.D. (2021). A tobacco ringspot virus-based vector system for gene and microRNA function studies in cucurbits. Plant Physiol. Mar 25:kiab146..

1497. Gallaher, S.D., Craig, R.J., Ganesan, I., Purvine, S.O., McCorkle, S.R., Grimwood, J., Strenkert, D., Davidi, L., Roth, M.S., Jeffers, T.L., Lipton, M.S., Niyogi, K.K., Schmutz, J. Theg, S.M., Blaby-Haas, C.E. & Merchant, S.S. (2021). Widespread polycistronic gene expression in green algae. Proc. Natl. Acad. Sci. USA. 118(7):e2017714118.

1496. Gatina, D.Z., Garanina, E.E., Zhuravleva, M.N., Synbulatova, G.E., Mullakhmetova, A.F., Solovyeva, V.V., Kiyasov, A.P., Rutland, C.S., Rizvanov, A.A. & Salafutdinov, I.I. (2021). Proangiogenic effect of 2A-peptide based multicistronic recombinant constructs encoding VEGF and FGF2 growth factors. Int. J. Mol. Sci. 22(11):5922.

1495. Goldman, D.H., Livingston, N.M., Movsik, J., Wu, B. & Green, R. (2021). Live-cell imaging reveals kinetic determinants of quality control triggered by ribosome stalling. Mol. Cell 81, 1–11.

1494. González-Romero, M.E., Rivera, C., Cancino, K., Geu-Flores, F., Cosio, E.G., Ghislain, M. & Halkier. B.A. (2021). Bioengineering potato plants to produce benzylglucosinolate for improved broad-spectrum pest and disease resistance. Transgenic Res. doi: 10.1007/s11248-021-00255-w. Online ahead of print.

1493. Gualdani, R., Yuan, J.H., Effraim, P.R., Di Stefano, G., Truini, A., Cruccu, G., Dib-Hajj, S.D., Gailly, P. & Waxman, S.G. (2021). Trigeminal neuralgia TRPM8 mutation: enhanced activation, basal [Ca2+]i and menthol response. Neurol. Genet. Jan 11;7(1):e550.

1492. Hudson, A.M., Szabo, N.L., Loughran, G., Wills, N.M., Atkins, J.F. & Cooley, L. (2021). Tissue-specific dynamic codon redefinition in Drosophila. Proc. Natl. Acad. Sci. USA. 118(5):e2012793118.

1491. Jacobsen, R.I., Nair, R.R., Obenhaus, H.A. Donato, F., Slettmoen, T., Moser, M-B., Moser, E.I. (2021). All-viral tracing of monosynaptic inputs to single birthdate-defined neurons in the intact brain. bioRxiv preprint doi: https://doi.org/10.1101/2021.10.18.464781.

1490. Jadav, A. & Truong, K. (2021). Generation of stable cell lines using readthrough expression from lentiviral integration. Biotechnol Lett. doi: 10.1007/s10529-021-03186-y. Online ahead of print.

1489. Jang, H., Park, Y.H., Kim, M-H., You, J., Ko, J-H, & Lee, J.T. (2021). Surface characteristics of porous carbon derived from genetically designed transgenic hybrid poplar for electric double-layer capacitors. App. Surface Sci. 545:148978.

1488. Jeong, Y,S., Ku, H-K., Jung, Y-J., Kim, J.K., Lee, K.B., Kim, J-K., Lim, S-H., Lee, D. & Ha, S.H. (2021). 2A-linked bi-, tri-, and quad-cistrons for the stepwise biosynthesis of ß-carotene, zeaxanthin, and ketocarotenoids in rice endosperm. Metab. Eng. Commun. 12:e00166.

1487. Kaczmarek, M.B, Struszczyk-Swita, K., Xiao, M., Szczesna-Antczak, M., Antczak, T., Gierszewska, M., Steinbüchel, A. & Daroch, M. (2021). Polycistronic expression system for Pichia pastoris composed of chitino- and chitosanolytic enzymes. Fron. Bioeng. Biotech. 9:710922.

1486. Kandul, N.P., Belikoff, E.J., Liu, J., Buchman, A., Li, F., Yamamoto, A., Yang, T., Shriner, I., Scott, M.J. & Akbari, O.S. (2021). Genetically encoded CRISPR components yield efficient gene editing in the invasive pest Drosophila suzukii. CRISPR J. 4, 739-751.

1485. Khosla, A., Rodriguez-Furlan, C., Kapoor, S., Van Norman, J.M. & Nelson, D.C. (2021). A series of dual-reporter vectors for ratiometric analysis of protein abundance in plants. Plant Direct 4, e00231.

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1474. Müller, J.P., Scholl, S., Kunick, C. & Klempnauer, K.H. (2021). Expression of protein kinase HIPK2 is subject to a quality control mechanism that acts during translation and requires its kinase activity to prevent degradation of nascent HIPK2. Biochim. Biophys. Acta Mol. Cel.l Res. 1868(1):118894.

1473. Murakoshi, H. (2021). Optogenetic imaging of protein activity using two-photon fluorescence lifetime imaging microscopy. Optogenetics, 1293, 295-308.

1472. Ohta, T., Morikawa, Y., Sato, M., Konno, A., Hirai, H., Kurauchi, Y., Hisatsune, A., Katsuki, H. & Seki, T. (2021). Therapeutic potential of D-cysteine against in vitro and in vivo models of spinocerebellar ataxia. Exp. Neurol. 343:113791. Research Square https://doi.org/10.21203/rs.3.rs-598844/v1

1471. Philip, A.A. & Patton, J.T. (2021). Rotavirus as an expression platform of the SARS-CoV-2 spike protein. bioRxiv. 2021 Feb 18:2021.02.18.431835.

1470. Pleiner, T., Hazu, M., Tomaleri, G.P., Januszyk, K., Oania, R.S., Sweredoski, M.J., Moradian, A., Guna, A. & Voorhees, R.M. (2021). WNK1 is an assembly factor for the human ER membrane protein complex. Mol Cell. S1097-2765(21)00318-X.

1469. Rand, U., Kubsch, T., Kasmapour, B. and Cicin-Sain, L. (2021). A novel triple-fluorescent HCMV strain reveals gene expression dynamics and anti-herpesviral drug mechanisms. Front. Cell Infect. Microbiol. 10, 536150.

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1467. Rozen-Gagnon, K., Yi, S., Jacobson, E., Novack, S. & Rice, C.M. (2021). A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines. Sci Rep. 11(1):736.

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1465. Sadoine, M., Ishikawa, Y., Kleist, T.J., Wudick, M.M., Nakamura, M., Grossmann, G., Frommer, W.M. & Ho. C-H. (2021). Designs, applications, and limitations of genetically encoded fluorescent sensors to explore plant biology. Plant Physiol. 00, 1-19.

1464. Scarborough. A.M., Flaherty, J.N., Hunter, O.V., Liu, K., Kumar, A., Xing, C., Tu, B.P. & Conrad, N.K. (2021). SAM homeostasis is regulated by CFI(m)-mediated splicing of MAT2A. eLife 10:e64930.

1463. Schwirz, J., Yan, Y., Franta, Z. & Schetelig, M.F. (2021). Bicistronic expression and differential localization of proteins in insect cells and Drosophila suzukii using picornaviral 2A peptides. Insect Biochem. Mol. Biol. 119, 103324.

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1461. Shibata, A.C.E., Ueda, H.H., Eto, K., Onda, M., Sato, A., Ohba, T., Nabekura, J. & Murakoshi, H. (2021). Photoactivatable CaMKII induces synaptic plasticity in single synapses. Nat. Commun. 12(1):751.

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1452. Wang, M., Mou, C., Chen, M. & Chen, Z. (2021). Infectious recombinant Senecavirus A expressing novel reporter proteins. Appl. Microbiol. Biotechnol. 105, 2385-2397.

1451. Whittington, K.B., Prislovsky, A., Beaty, J., Albritton, L., Radic, M. & Rosloniec, E.F. (2021). CD8+ T Cells expressing an HLA-DR1 chimeric antigen receptor target autoimmune CD4+ T cells in an antigen-specific manner and inhibit the development of autoimmune arthritis. J. Immunol. ji2100643.

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2020

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1440. Bohlender, L.L., Parsons, J., Hoernstein, S.N.W., Rempfer, C., Ruiz-Molina, N., Lorenz, T., Rodríguez Jahnke, F., Figl, R., Fode, B., Altmann, F., Reski, R. & Decker, E.L. (2020). Stable Protein Sialylation in Physcomitrella. Front. Plant Sci. 11:610032.

1439. Cai, L., Hu, H., Duan, H., Li, Y., Zou, Z., Luo, K., Zhang, Z., Yang, J., Jin, J., Chen, Y., Ke, Z., Fang, Z., Liu, Q., Hong, X., Hu, S. & Liu, B. (2020). The construction of a new oncolytic herpes simplex virus expressing murine interleukin-15 with gene-editing technology. J. Med. Virol. doi: 10.1002/jmv.25691. Online ahead of print.

1438. Chiale, C., Yarovinsky, T.O., Mason, S.W., Madina, B.R., Menon, M., Krady, M.M., Moshkani, S., Chattopadhyay Pal, A., Almassian, B., Rose, J.K., Robek, M.D. & Nakaar, V. (2020). Modified alphavirus-vesiculovirus hybrid vaccine vectors for homologous prime-boost immunotherapy of chronic hepatitis B. Vaccines (Basel) 8(2):279.

1437. Chu, I-R., Pan, R-L., Yang, C-S. and Wu, L-C. (2020). A doxycycline-inducible C17.2 neural stem cell-based combination of differentiation and suicide gene therapy for an in vitro tumorigenic C6 glioma model. Biotech. Biotechnological Equipment 34, 986-998.

1436. Costello, A., Lao, N.T., Barron, N. & Clynes, M. (2020). Reinventing the wheel: synthetic circular RNAs for mammalian cell engineering. Trends Biotechnol. 38, 217-230.

1435. Crowther, M, D., Dolton, G., Legut, M., Caillaud, M, E., Lloyd, A., Attaf, M., Galloway, S., Rius, C., Farrell, C, P., Szomolay, B., Ager, A., Parker, A, L., Fuller, A., Donia, M., McCluskey, J., Rossjohn, J., Svane, I, M., Phillips, J, D. & Sewell, A, K. (2020). Genome-wide CRISPR-Cas9 screening reveals ubiquitous T cell cancer targeting via the monomorphic MHC class I-related protein MR1. Nat. Immunol. 21,178-185.

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1432. Dehghani, J., Adibkia, K., Movafeghi, A., Pourseif, M, M. & Omidi, Y. (2020). Designing a new generation of expression toolkits for engineering of green microalgae; robust production of human interleukin-2. Bioimpacts 10, 257-266.

1431. Del Papa, J., Clarkin, R, G. & Parks, R, J. (2020). Use of cell fusion proteins to enhance adenoviral vector efficacy as an anti-cancer therapeutic. Cancer Gene Therapy 2020 Jul 1. doi: 10.1038/s41417-020-0192-9

1430. Dhamija, S., Yang, C.M., Seiler, J., Myacheva, K., Caudron-Herger, M., Wieland, A., Abdelkarim, M., Sharma, Y., Riester, M., Groß, M., Maurer, J. & Diederichs, S. (2020). A pan-cancer analysis reveals nonstop extension mutations causing SMAD4 tumour suppressor degradation. Nat. Cell Biol. 22, 999-1010.

1429. Dorrington, R.A, Jiwaji, M., Awando, J.A & de Bruyn, M-M. (2020). Advances in Tetravirus research: new insight into the infectious virus life Cycle and an expanding host range. Curr. Issues Mol. Biol. 34, 145-162.

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1427. Farnworth, M, S., Eckermann, K, N., Ahmed, K, M., Mühlen, D, S., He, B. & Bucher, G. (2020). The red flour beetle as model for comparative neural development: Genome editing to mark neural cells in tribolium brain development. Methods Mol Biol. 2047, 191-217.

1426. Farrera-Sal, M., de Sostoa, J., Nuñez-Manchón, E., Moreno, R., Fillat, C., Bazan-Peregrino, M. & Alemany, R. (2020). Arming oncolytic adenoviruses: Effect of insertion site and splice acceptor on transgene expression and viral fitness. Int. J. Mol. Sci. 21(14):5158.

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1424. Fuller, J., Álvarez-Rodríguez, B., Todd, E., Mankouri, J., Hewson, R. & Barr, J, N. (2020). Hazara Nairovirus Requires COPI Components in both Arf1-Dependent and Arf1-Independent Stages of Its Replication Cycle. J. Virol. 94, e00766-20.

1423. Garcia-Gonzalez, I., Mühleder, S., Fernández-Chacón, M. & Benedito, R. (2020). Genetic tools to study cardiovascular biology. Front Physiol. 11:1084.

1422. Gioseffi, A., Hamerly, T., Van, K., Zhang, N., Dinglasan, R.R., Yates, P.A. & Kima, P.E. (2020). Leishmania-infected macrophages release extracellular vesicles that can promote lesion development. Life Sci Alliance 3(12):e202000742.

1421. Grabert, K., Sehgal, A., Irvine, K.M., Wollscheid-Lengeling, E., Rojo, R., Ozdemir, D., Stables, J., Ryan, M.D., Adamson, A., Humphreys, N.E., Luke, G.A., Sandrock, C.J., Verkasalo, V., Pettit, A.L., Mueller, W., Hohenstein, P., Pridans, C. & Hume, D.A. (2020). A transgenic line that reports CSF1R protein expression provides a definitive marker for the mouse mononuclear phagocyte system. J. Immunol. 205, 3154-3166..

1420. Guseva, S., Milles, S., Jensen, M, R., Kleman, J., Maurin, D., Ruigrok, R. & Blackledge, M. (2020). Measles virus nucleo- and phosphoproteins form liquid-like phase-separated compartments that promote nucleocapsid assembly. Sci Adv. 6, eaaz7095.

1419. Hao, J., Yang, X., Zhang, C., Zhang, X.T., Shi, M., Wang, S.H., Mi, L., Zhao, Y.T., Cao, H. & Wang, Y. (2020). KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells. Cell Prolif. 53(11):e12914.

1418. Han, P., Shichino, Y., Schneider-Poetsch, T., Mito, M., Hashimoto, S., Udagawa, T., Kohno, K., Yoshida, M., Mishima, Y., Inada, T. & Iwasaki, S. (2020). Genome-wide Survey of Ribosome Collision. Cell Reports. 31, (5).

1417. Hannemann, H. (2020). Viral replicons as valuable tools for drug discovery. Drug Discov. Today. 25, 1026-1033.

1416. He, Y., Zhang, T., Sun, H., Zhan, H. & Zhao, Y. (2020). A reporter for noninvasively monitoring gene expression and plant transformation. Hortic. Res. 7:152.

1415. Hinz, S, C., Elter, A., Grzeschik, J., Habermann, J., Becker, B. & Kolmar, H. (2020). Simplifying the detection of surface presentation levels in yeast surface display by intracellular tgfp expression. Methods Mol. Biol. 211-222.

1414. Holmes, D.R., Morbitzer, R., Wunderlich, M., Sun, H., El Kasmi, F., Schneeberger, K. & Lahaye, T. (2020). A shortcut in forward genetics: concurrent discovery of utant phenotype and causal mutation in Arabidopsis M2 families via MAD mapping. bioRxiv. doi: 10.1101/2020.06.29.177808.

1413. Huang, J., Wang, A., Huang, C., Sun, Y., Song, B., Zhou, R. & Li, L. (2020). Generation of marker-free pbd-2 knock-in pigs using the CRISPR/Cas9 and Cre/loxP systems. Genes (Basel) 11(8):951.

1412. Ibrahim, S., Harris-Kawano, A., Haider, I., Mirmira, R, G., Sims, E, K. & Anderson, R, M. (2020). A novel Cre-enabled tetracycline-inducible transgenic system for tissue-specific cytokine expression in the zebrafish: CETI-PIC3. Dis. Model Mech. 13(6).

1411. Jaffri, S.A., Yan, Y., Schwirz, J. & Schetelig, M.F. (2020). Functional characterization of the Drosophila suzukii pro-apoptotic genes reaper, head involution defective and grim. Apoptosis 25, 864-874.

1410. Ji, Y., Li, B., Qiao, M., Li, J., Xu, H., Zhang, L. & Zhang, X. (2020). Advances on the in vivo and in vitro glycosylations of flavonoids. Appl. Microbiol. Biotechnol. 104, 6587-6600.

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