2A in Biomedicine & Biotechnology
Research papers using 2A, plus reviews discussing the use of 2A
Comment upon Sommer et al., (2009). iPS cell generation using a single lentiviral stem cell cassette. Stem Cells 27, 543-549.
'Converting Adult Somatic Cells to Pluripotent Stem Cells using a Single Virus' - Physorg.com
Comment upon Holst et al., (2006). Rapid analysis of T-cell selection in vivo using T cell-receptor retrogenic mice. Nature Methods 3, 191-197.
'Retroviral TCR Gene Transduction: 2A for Two'. Bosselut, R. (2006). News & Views, Nature Methods 3, 162-164.
Comment upon Fang et al., (2005). Stable antibody expression at therapeutic levels using the 2A peptide. Nature Biotechnol. 23, 584-590.
'Therapeutic Antibody Gene Transfer' - 'News and Views' discussion the technology for gene therapy in Nature Biotechnology.
'Viral 2A Peptide Stabilizes Monoclonal Antibody at Therapeutic Levels' Gene Therapy Weekly
Comment upon Szymczak et al., (2004). Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector. Nature Biotechnol. 22, 589-94.
'Put all your Genes in One Basket ' - a highlight of the technology for gene therapy in Nature Methods
'Caught in Translation ' - a highlight of the technology for gene therapy in Nature Immunology
'Multiple Gene products from a single vector; 'self-cleaving 2A peptides' Gene Therapy 11, 1673-1674 (2004).
'Virus Trick Could Turn Cells into Drug Factories' Better Humans
'Genetic Trick Adapted From Viruses Makes Gene Therapy Vectors More Versatile'
Science Daily ..Innovations Report...Charity Wire...St. Judes Childrens Research Hospital.. News-Medical.Net.... Techmonitor.net..bio.com
'Gene Therapy Enhanced by Virus Peptides' .....MedMarkets
2011
487. Akitake, C.M., Macurak, M., Halpern, M.E. & Goll, M.G. (2011). Transgenerational analysis of transcriptional silencing in zebrafish. Dev. Biol. 352, 191-201.
486. Artegiani, B., Lindemann, D. & Calegari, F. (2011). Overexpression of cdk4 and cyclinD1 triggers greater expansion of neural stem cells in the adult mouse brain. J. Exp. Med. 208, 937-948.
485. Boersma, M.C., Dresselhaus, E.C., De Biase, L.M., Mihalas, A.B., Bergles, D.E. & Meffert, M.K. (2011). A requirement for nuclear factor-{kappa}B in developmental and plasticity-associated synaptogenesis. J. Neurosci. 31, 5414-5425.
484. Bonini, C., Brenner, M.K., Heslop, H.E. & Morgan, R.A. (2011). Genetic modification of T cells. Biol. Blood Marrow Transplant. 17, S15-20.
483. Bower, D.V., Sato, Y. & Lansford, R. (2011). Dynamic lineage analysis of embryonic morphogenesis using transgenic quail and 4D multispectral imaging. Genesis 49, 619-463.
482. Broxmeyer, H.E., Lee, M.R., Hangoc, G., Cooper, S., Prasain, N., Kim, Y.J., Mallett, C., Ye, Z., Witting, S., Cornetta, K., Cheng, L. & Yoder, M.C. (2011). Hematopoietic stem/progenitor cells, generation of induced pluripotent stem cells, and isolation of endothelial progenitors from 21- to 23.5-year cryopreserved cord blood. Blood 117, 4773-4777
481. Camper, N., Byrne, T., Burden, R.E., Lowry, J., Gray, B., Johnston, J.A., Migaud, M.E., Olwill, S.A., Buick, R.J. & Scott, C.J. (2011). Stable expression and purification of a functional processed Fab' fragment from a single nascent polypeptide in CHO cells expressing the mCAT-1 retroviral receptor. J. Immunol. Methods 372, 30-41.
480. Chinnasamy, N., Wargo, J.A., Yu, Z., Rao, M., Frankel, T.L., Riley, J.P., Hong, J.J., Parkhurst, M.R., Feldman, S.A., Schrump, D.S., Restifo, N.P., Robbins, P.F., Rosenberg, S.A & Morgan, R.A. (2011). A TCR targeting the HLA-A*0201-restricted epitope of MAGE-A3 recognizes multiple epitopes of the MAGE-A antigen superfamily in several types of cancer. J. Immunol. 186, 685-696.
479. Clyde, K. & Glaunsinger, B.A. (2011). Deep sequencing reveals direct targets of gammaherpesvirus-induced mRNA decay and suggests that multiple mechanisms govern cellular transcript escape. PLoS One. 6, e19655.
478. Deng, W., Yang, D., Zhao, B., Ouyang, Z., Song, J., Fan, N., Liu, Z., Zhao, Y., Wu, Q., Nashun, B., Tang, J., Wu, Z., Gu, W. & Lai, L. (2011). Use of the 2A peptide for generation of multi-transgenic pigs through a single round of nuclear transfer. PLoS One 6, e19986
477. Di Stasi, A., Tey, S.K., Dotti, G., Fujita, Y., Kennedy-Nasser, A., Martinez, C., Straathof, K., Liu, E., Durett, A.G., Grilley, B., Liu, H., Cruz, C.R., Savoldo, B., Gee, A.P., Schindler, J., Krance, R.A., Heslop, H.E., Spencer, D.M., Rooney, C.M. & Brenner, M.K. (2011). Inducible apoptosis as a safety switch for adoptive cell therapy. New England J. Med. 365, 1673-1683.
476. Fisicaro, N., Londrigan, S.L., Brady, J.L., Salvaris, E., Nottle, M.B., O'Connell, P.J., Robson, S.C., d'Apice, A.J., Lew, A.M. & Cowan, P.J. (2011). Versatile co-expression of graft-protective proteins using 2A-linked cassettes. Xenotransplantation 18, 121-130.
475. Fujita, M., Cha, Y.R., Pham, V.N., Sakurai, A., Roman, B.L., Gutkind, J.S. & Weinstein, B.M. (2011). Assembly and patterning of the vascular network of the vertebrate hindbrain. Development 138, 1705-1715.
474. González, F., Boué, S. & Belmonte, J.C. (2011). Methods for making induced pluripotent stem cells: reprogramming à la carte. Nat. Rev. Genet. 12, 231-242
473. Gonzalez, M., Martin-Ruiz, I., Jimenez, S., Pirone, L., Barrio, R. & Sutherland, J.D. (2011). Generation of stable Drosophila cell lines using multicistronic vectors. Scentific Reports 1 Article Number: 75 DOI: 10.1038/srep00075 .
472. Heldens, L., Hensen, S.M., Onnekink, C., van Genesen, S.T., Dirks, R.P. & Lubsen, N.H. (2011). An atypical unfolded protein response in heat shocked cells. PLoS One 6, e23512.
471. Hertel, F., Switalski, A., Mintert-Jancke, E., Karavassilidou, K., Bender, K., Pott, L. & Kienitz, M.C. (2011) . A genetically encoded tool kit for manipulating and monitoring membrane phosphatidylinositol 4,5-bisphosphate in intact cells. PLoS One 6(6):e20855.
470. Li, H-y., Sun, Y., Zhang, X-j., Chang, T-m., Wang, X-p., He, F., Huang, J. & Qiu, H-j. (2011). Generation and immunogenicity of a recombinant adenovirus co-expressing the E2 protein of classical swine fever virus and the GP5 protein of porcine reproduction and respiratory syndrome virus. Agri. Sci. In China 10, 1781-1791.
469. Husseiny, M.I. & Lacey, S.F. (2011). Development of infectious recombinant BK virus. Virus Res. 161, 150-161.
468. Khodosevich, K., Watanabe, Y. & Monyer, H. (2011). EphA4 preserves postnatal and adult neural stem cells in an undifferentiated state in vivo. J. Cell Sci. 15,1268-1279.
467. Kerrigan, J.J., Xie, Q., Ames, R.S. & Lu, Q. (2011). Production of protein complexes via co-expression. Protein Expr Purif. 75, 1-14.
466. Kim, H.Y., Tsai, S., Lo, S.C., Wear, D.J. & Izadjoo, M.J. (2011). Production and characterization of chimeric monoclonal antibodies against Burkholderia pseudomallei and B. mallei using the DHFR expression system. PLoS One 6(5):e19867.
465. Kim, J.H., Lee, S.R., Li, L.H., Park, H.J., Park, J.H., Lee, K.Y., Kim, M.K., Shin, B.A. & Choi, S.Y. (2011). High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice. PLOS ONE 6, e18556.
464. Kovalskaya, N., Zhao, Y. & Hammond, R.W. (2011). Antibacterial and antifungal activity of a snakin-defensin hybrid protein expressed in tobacco and potato plants. Open Plant Sci. J. 5, 29-42.
463. Lebbink, R.J., Lowe, M., Chan, T., Khine, H., Wang, X. & McManus, M.T. (2011). Polymerase II promoter strength determines efficacy of microRNA adapted shRNAs. PLoS One 6 :e26213. Epub 2011 Oct 21.
462. Lin, Y., Cheung, P., Roth, J.C., Wilson, D.L. & Gerson S.L. (2011). Imaging stem cell-derived persistent foci after in vivo selection of lentiviral MGMT-P140K transduced murine bone marrow cells. Mol. Ther. 19, 1342-1352.
461. Ma, J., Guo, L., Fiene, S.J., Anson, B.D., Thomson, J.A., Kamp, T..J, Kolaja, K.L., Swanson, B.J. & January, C.T. (2011). High purity human-induced pluripotent stem cell-derived cardiomyocytes: electrophysiological properties of action potentials and ionic currents. Am. J. Physiol. Heart Circ. Physiol. 301, H2006-2017.
460. Mackanos, M.A. & Contag, C.H. (2011). Pulse duration determines levels of Hsp70 induction in tissues following laser irradiation. J. Biomed. Opt. 16:078002.
459. Maier, P., von Kalle, C. & Laufs, S. (2011). Retroviral vectors for gene therapy. Future Microbiol. 5, 1507-1523.
458. Montserrat, N., Garreta, E., González, F., Gutiérrez, J., Eguizábal, C., Ramos, V., Borrós, S. & Izpisua Belmonte, J.C. (2011). Simple generation of human induced pluripotent stem cells using poly-{beta}-amino esters as the non-viral gene delivery system. J. Biol. Chem. 286, 12417-12428.
457. Nefzger, C.M., Haynes, J.M. & Pouton, C.W. (2011). Directed expression of gata2, mash1, and foxa2 synergize to induce the serotonergic neuron phenotype during in vitro differentiation of embryonic stem cells. Stem Cells 29, 928-939.
456. Ochi, T., Fujiwara, H. & Yasukawa, M. (2011). Requisite considerations for successful adoptive immunotherapy with engineered T-lymphocytes using tumor antigen-specific T-cell receptor gene transfer. Expert Opin. Biol. Ther. 11, 699-713.
455. Osakada, F., Mori, T., Cetin, A.H., Marshel, J.H., Virgen, B. & Callaway, E.M. (2011). New rabies virus variants for monitoring and manipulating activity and gene expression in defined neural circuits. Neuron 71, 617-631
454. Osborn, M.J., DeFeo, A.P., Blazar, B.R. & Tolar, J. (2011). Synthetic zinc finger nuclease design and rapid assembly. Hum. Gene Ther. 22, 1155-1165.
453. Pincha, M., Salguero, G., Wedekind, D., Sundarasetty, B.S., Lin, A., Kasahara, N., Brugman, M.H., Jirmo, A.C., Modlich, U., Gutzmer, R., Büsche, G., Ganser, A. & Stripecke, R. (2011). Lentiviral vectors for induction of self-differentiation and conditional ablation of dendritic cells. Gene Ther. 18, 750-764.
452. Pollier, J., Moses, T. & Goossens, A. (2011).Combinatorial biosynthesis in plants: A (p)review on its potential and future exploitation. Natural Product Rep. 28, 1897-1916.
451. Quirin, C., Rohmer, S., Fernández-Ulibarri, I., Behr, M., Hesse, A., Engelhardt, S., Erbs, P., Enk, A.H. & Nettelbeck, D.M. (2011). Selectivity and efficiency oflate transgene expression by transcriptionally targeted oncolytic adenoviruses are dependent on the transgene insertion strategy. Hum. Gene Ther. 22, 389-404.
450. Robbins, P.F., Morgan, R.A., Feldman, S.A., Yang, J.C., Sherry, R.M., Dudley, M.E., Wunderlich, J.R., Nahvi, A.V., Helman, L.J., Mackall, C.L., Kammula, U.S., Hughes, M.S., Restifo, N.P., Raffeld, M., Lee, C.C., Levy, C.L., Li, Y.F., El-Gamil, M., Schwarz, S.L., Laurencot, C. & Rosenberg, S.A. (2011). Tumor regression in patients with metastatic synovial cell sarcoma and melanoma using genetically engineered lymphocytes reactive with NY-ESO-1. J. Clin. Oncol. 29, 917-924
449. Robinson, M., Tian, Y., Delaney, W.E. 4th & Greenstein, A.E. (2011). Preexisting drug-resistance mutations reveal unique barriers to resistance for distinct antivirals. Proc. Natl. Acad. Sci. U.S.A. 108, 10290-10295.
448. Rogan, S.C. & Roth, B.L. (2011). Remote control of neuronal signaling. Pharmacol Rev. 63, 291-315.
447. Sempere, R.N., Gómez, P., Truniger, V. & Aranda, M.A. (2011). Development of expression vectors based on pepino mosaic virus. Plant Methods 7, 6.
446. Sharma, N.R., Mateu, G., Dreux, M., Grakoui, A., Cosset, F.L. & Melikyan, G.B. (2011). Hepatitis C virus is primed by CD81 protein for low pH-dependent fusion. J. Biol. Chem. 286, 30361-30376.
445. Shi, C., Jia, T., Mendez-Ferrer, S., Hohl, T.M., Serbina, N.V., Lipuma, L., Leiner, I., Li, M.O., Frenette, P.S. & Pamer, E.G. (2011). Bone marrow mesenchymal stem and progenitor cells induce monocyte emigration in response to circulating Toll-like receptor ligands. Immunity 34, 590-601.
444. Shimada, I.S., Borders, A., Aronshtam, A. & Spees, J.L. (2011). Proliferating reactive astrocytes are regulated by Notch-1 in the peri-infarct area after stroke. Stroke 42, 3231-3237.
443. Shioi, G., Kiyonari, H., Abe, T., Nakao, K., Fujimori, T., Jang, C.W., Huang, C.C., Akiyama, H., Behringer, R.R. & Aizawa, S. (2011). A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging. Genesis 49, 570-578.
442. Singhal, R., Deng, X., Chenchik, A.A. & Kandel, E.S. (2011). Long-distance effects of insertional mutagenesis. PLoS ONE 6, e15832.
441. Sia, K.C., Huynh, H., Chinnasamy, N., Hui, K.M. & Lam, P.Y.P. (2011). Suicidal gene therapy in the effective control of primary human hepatocellular carcinoma as monitored by noninvasive bioimaging. Gene Therapy [E-pub ahead of print].
440. Suter, D.M., Molina, N., Gatfield, D., Schneider, K., Schibler, U. & Naef, F. (2011). Mammalian genes are transcribed with widely different bursting kinetics. Science 332, 472-474.
439. Tatineni, S., McMechan, A.J., Hein, G.L. & French, R. (2011). Efficient and stable expression of GFP through Wheat streak mosaic virus-based vectors in cereal hosts using a range of cleavage sites: formation of dense fluorescent aggregates for sensitive virus tracking. Virology 410, 268-281.
438. van Loenen, M.M., de Boer, R., Hagedoorn, R.S., van Egmond, E.H., Falkenburg, J.H. & Heemskerk, M.H. (2011). Optimization of the HA-1-specific T-cell receptor for gene therapy of hematologic malignancies. Haematologica 96, 477-481
437. Verrier, J.D., Madorsky, I., Coggin, W.E., Geesey, M., Hochman, M., Walling, E., Daroszewski, D., Eccles, K.S., Ludlow, R. & Semple-Rowland, S.L. (2011). Bicistronic lentiviruses containing a viral 2A cleavage sequence reliably co-express two proteins and restore vision to an animal model of LCA1. PLoS ONE 6, e20553.
436. Wang, Q.J., Hanada, K., Feldman, S.A., Zhao, Y., Inozume, T. & Yang, J.C. (2011). Development of a genetically-modified novel T-cell receptor for adoptive cell transfer against renal cell carcinoma. J Immunol Methods 366, 43-51.
435. Wang, X., Berger, C., Wong, C.W., Forman, S.J., Riddell, S.R. & Jensen, M.C. (2011). Engraftment of human central memory-derived effector CD8+ T cells in immunodeficient mice. Blood 117, 1888-1898
434. Wu, H.L., Wang, Y., Zhang, P., Li, S.F., Chen, X., Chen, Y.K., Li, J.G., Yang, S.M., Su, Y.P., Wang, J.P. & Chen, B. (2011). Reversible immortalization of rat pancreatic ß cells with a novel immortalizing and tamoxifen-mediated self-recombination tricistronic vector. J. Biotechnol. 151, 231-241.
433. Yi, Z., Sperzel, L., Nürnberger, C., Bredenbeek, P.J., Lubick, K.J., Best, S.M., Stoyanov, C.T., Law, L.M.J. Yuan, Z. Rice, C.M. & MacDonald, M.R. (2011). Identification and characterization of the host protein DNAJC14 as a broadly active flavivirus replication modulator. PLoS Pathog. 7, e1001255.
2010
432. Bednarczyk, D. (2010). Fluorescence-based assays for the assessment of drug interaction with the human transporters OATP1B1 and OATP1B3. Anal. Biochem. 405, 50-58.
431. Benabdallah, B.F., Allard, E., Yao, S., Friedman, G., Gregory, P.D., Eliopoulos, N., Fradette, J., Spees, J.L., Haddad, E., Holmes, M.C. & Beauséjour, C.M. (2010). Targeted gene addition to human mesenchymal stromal cells as a cell-based plasma-soluble protein delivery platform. Cytotherapy 12, 394-399
430. Bendle, G.M., Linnemann, C., Hooijkaas, A.I., Bies, L., de Witte, M.A., Jorritsma, A., Kaiser, A.D., Pouw, N., Debets, R., Kieback, E., Uckert, W., Song, J.Y., Haanen, J.B. & Schumacher, T.N. (2010). Lethal graft-versus-host disease in mouse models of T cell receptor gene therapy. Nat. Med. 16, 565-570.
429. Bennett, M.S., Joseph, A., Ng, H.L., Goldstein, H. & Yang, O.O. (2010). Fine-tuning of T-cell receptor avidity to increase HIV epitope variant recognition by cytotoxic T lymphocytes. AIDS 24, 2619-2628.
428. Bonderoff, J.M. & Lloyd, R.E. (2010). Time-dependent increase in ribosome processivity. Nuc. Acids Res. 38, 7054-7067.
427. Brusko, T.M., Koya, R.C., Zhu, S., Lee, M.R., Putnam, A.L., McClymont, S.A., Nishimura, M.I., Han, S., Chang, L.J., Atkinson, M.A., Ribas, A. & Bluestone, J.A. (2010). Human antigen-specific regulatory T cells generated by T cell receptor gene transfer. PLoS One 5(7):e11726.
426. Burton, A.R., Baquet, Z., Eisenbarth, G.S., Tisch, R., Smeyne, R., Workman, C.J. & Vignali, D.A. (2010). Central nervous system destruction mediated by glutamic acid decarboxylase-specific CD4(+) T cells. J. Immunol. 184, 4863-4870.
425. Casales, E., Aranda, A., Quetglas, J.I., Ruiz-Guillen, M., Rodriguez-Madoz, J.R., Prieto, J. & Smerdou, C. (2010). A novel system for the production of high levels of functional human therapeutic proteins in stable cells with a Semliki Forest virus noncytopathic vector. New Biotech. 27, 138-148.
424. Coccoris, M., Straetemans, T., Govers, C., Lamers, C., Sleijfer, S. & Debets, R. (2010). T cell receptor (TCR) gene therapy to treat melanoma: lessons from clinical and preclinical studies. Expert Opin Biol Ther. 10, 547-562
423. Davis, J.L., Theoret, M.R., Zheng, Z., Lamers, C.H., Rosenberg, S.A. & Morgan, R.A. (2010). Development of human anti-murine T-cell receptor antibodies in both responding and nonresponding patients enrolled in TCR gene therapy trials. Clin Cancer Res. 16, 5852-5861.
422. Dirks, R.P., van Geel, R., Hensen, S.M., van Genesen, S.T. & Lubsen, N.H. (2010). Manipulating heat shock factor-1 in Xenopus tadpoles: neuronal tissues are refractory to exogenous expression. PLoS One 5(4):e10158.
421. de Felipe, P., Luke, G.A., Brown, J.D. & Ryan, M.D. (2010). Inhibition of 2A-mediated 'cleavage' of certain artificial polyproteins bearing N-terminal signal sequences. Biotechnol. J. 5, 213-223.
420. Edmonds, T.G., Ding, H., Yuan, X., Wei, Q., Smith, K.S., Conway, J.A., Wieczorek, L., Brown, B., Polonis, V., West, J.T., Montefiori, D.C., Kappes, J.C. & Ochsenbauer, C. (2010). Replication competent molecular clones of HIV-1 expressing Renilla luciferase facilitate the analysis of antibody inhibition in PBMC. Virology 408, 1-13.
419. Frankel, T.L., Burns, W.R., Peng, P.D., Yu, Z., Chinnasamy, D., Wargo, J.A., Zheng, Z., Restifo, N.P., Rosenberg, S.A. & Morgan, R.A. (2010). Both CD4 and CD8 T cells mediate equally effective in vivo tumor treatment when engineered with a highly avid TCR targeting tyrosinase. J Immunol. 184, 5988-5998.
418. Freel, S,A., Lamoreaux, L., Chattopadhyay, P.K., Saunders, K., Zarkowsky, D., Overman, R.G., Ochsenbauer, C., Edmonds, T.G., Kappes, J.C., Cunningham, C.K., Denny, T.N., Weinhold, K.J., Ferrari, G., Haynes, B.F., Koup, R.A., Graham, B.S., Roederer, M. & Tomaras, G.D. (2010). Phenotypic and functional profile of HIV-inhibitory CD8 T cells elicited by natural infection and heterologous prime/boost vaccination. J. Virol. 84, 4998-5006.
417. Goedhart, J., van Weeren, L., Hink, M.A., Vischer, N.O., Jalink, K., Gadella, T.W. Jr. (2010). Bright cyan fluorescent protein variants identified by fluorescence lifetime screening. Nat Methods. 7, 137-139
416. Goff, S.L., Johnson, L.A., Black, M.A., Xu, H., Zheng, Z., Cohen, C.J., Morgan, R.A., Rosenberg, S.A. & Feldman. S.A. (2010). Enhanced receptor expression and in vitro effector function of a murine-human hybrid MART-1-reactive T cell receptor following a rapid expansion. Cancer Immunol Immunother. 59, 1551-1560.
415. Gradinaru, V., Zhang, F., Ramakrishnan, C., Mattis, J., Prakash, R., Diester, I., Goshen, I., Thompson, K.R. & Deisseroth, K. (2010). Molecular and cellular approaches for diversifying and extending optogenetics. Cell 141, 154-165.
414. Ha, S.H., Liang, Y.S., Jung, H., Ahn, M.J., Suh, S.C., Kweon, S.J., Kim, D.H., Kim, Y.M. & Kim, J.K. (2010). Application of two bicistronic systems involving 2A and IRES sequences to the biosynthesis of carotenoids in rice endosperm. Plant Biotechnol J. 8, 928-938.
413. Ha, S.P., Klemen, N.D., Kinnebrew, G.H., Brandmaier, A.G., Marsh, J., Hangoc, G., Palmer, D.C., Restifo, N.P., Cornetta, K., Broxmeyer, H.E. & Touloukian, C.E. (2010). Transplantation of mouse HSCs genetically modified to express a CD4-restricted TCR results in long-term immunity that destroys tumors and initiates spontaneous autoimmunity. J. Clin. Invest. 120, 4273-4288.
412. Haque, R., Lei, F., Xiong, X., Wu, Y & Song, J. (2010). FoxP3 and Bcl-xL cooperatively promote regulatory T cell persistence and prevention of arthritis development. Arthritis Research & Therapy 12, R66-77.
411. Haubensak, W., Kunwar, P.S., Cai, H., Ciocchi, S., Wall, N.R., Ponnusamy, R., Biag, J., Dong, H.W., Deisseroth, K., Callaway, E.M., Fanselow, M.S., Lüthi, A. & Anderson, D.J. (2010). Genetic dissection of an amygdala microcircuit that gates conditioned fear. Nature 468, 270-276
410. Hinrichs, C.S., Borman, Z.A., Gattinoni, L., Yu, Z., Burns, W.R., Huang, J., Klebanoff, C.A., Johnson, L.A., Kerkar, S.P., Yang, S., Muranski, P., Palmer, D.C., Scott, C.D., Morgan, R.A., Robbins, P.F., Rosenberg, S.A. & Restifo, N.P. (2010).Human effector CD8+ T cells derived from naive rather than memory subsets possess superior traits for adoptive immunotherapy. Blood. 117, 808-814.
409. Hu, W.G., Phelps, A.L., Jager, S., Chau, D., Hu, C.C., O'Brien, L.M., Perkins, S.D., Gates, A.J., Phillpotts, R.J. & Nagata, L.P. (2010). A recombinant humanized monoclonal antibody completely protects mice against lethal challenge with Venezuelan equine encephalitis virus. Vaccine 28, 5558-5564.
408. Hudecek, M., Schmitt, T.M., Baskar, S., Lupo-Stanghellini, M.T., Nishida, T., Yamamoto, T.N., Bleakley, M., Turtle, C.J., Chang, W.C., Greisman, H.A., Wood, B., Maloney, D.G., Jensen, M.C., Rader, C. & Riddell, S.R. (2010). The B-cell tumor-associated antigen ROR1 can be targeted with T cells modified to express a ROR1-specific chimeric antigen receptor. Blood 116, 4532-4541.
407. Kerkar, S.P., Muranski, P., Kaiser, A., Boni, A., Sanchez-Perez, L., Yu, Z., Palmer, D.C., Reger, R.N., Borman, Z.A., Zhang, L., Morgan, R.A., Gattinoni, L., Rosenberg, S.A., Trinchieri, G. & Restifo, N.P. (2010). Tumor-specific CD8+ T cells expressing interleukin-12 eradicate established cancers in lymphodepleted hosts. Cancer Res. 70, 6725-6734.
406. Kieback, E. & Uckert, W. (2010). Enhanced T cell receptor gene therapy for cancer. Expert Opin Biol Ther. 10, 749-762.
405. Kochenderfer, J.N., Wilson, W.H., Janik, J.E., Dudley, M.E., Stetler-Stevenson, M., Feldman, S.A., Maric, I., Raffeld, M., Nathan, D.A., Lanier, B.J., Morgan, R.A. & Rosenberg, S.A. (2010). Eradication of B-lineage cells and regression of lymphoma in a patient treated with autologous T cells genetically engineered to recognize CD19. Blood 116, 4099-4102.
404. Kuhns, M.S., Girvin, A.T., Klein, L.O., Chen, R., Jensen, K.D., Newell, E.W., Huppa, J.B., Lillemeier, B.F., Huse, M., Chien, Y.H., Garcia, K.C. & Davis, M.M. (2010). Evidence for a functional sidedness to the alphabetaTCR. Proc. Natl. Acad. Sc.i USA 107, 5094-5099.
403. Jang, C.W., Gao, L., Dickinson, M.E. & Behringer, R.R. (2010). Bmp4-directed nuclear cyan fluorescent protein provides a tool for live imaging and reveals cellular resolution of Bmp4 expression patterns during embryogenesis. Int. J. Dev. Biol. 54, 931-938.
402. Jasenosky, L.D., Neumann, G. & Kawaoka, Y. (2010). Minigenome-based reporter system suitable for high-throughput screening of compounds able to inhibit Ebolavirus replication and/or transcription. Antimicrobial Agents and Chemother. 54, 3007-3010.
401. Jazirehi, A.R., Baritaki, S., Koya, R.C., Bonavida, B. & Economou, J.S. (2010). Molecular mechanism of MART-1+/A*0201+ human melanoma resistance to specific CTL-killing despite functional tumor-CTL interaction. Cancer Res. 2010 Dec 15. [Epub ahead of print].
400. Jia, F., Wilson, K.D., Sun, N., Gupta, D.M., Huang, M., Li, Z., Panetta, N.J., Chen, Z.Y., Robbins, R.C., Kay, M.A., Longaker, M.T. & Wu, J.C. (2010). A nonviral minicircle vector for deriving human iPS cells. Nat. Methods 7,197199
399. Jincho, Y., Araki, R., Hoki, Y., Tamura, C., Nakamura, M., Ando, S., Kasama, Y. & Abe, M. (2010). Generation of genome integration-free induced pluripotent stem cells from fibroblasts of C57BL/6 mice without c-Myc transduction. J. Biol. Chem. 285, 26384-26389
398. Joseph, A., Zheng, J.H., Chen, K., Dutta, M., Chen, C., Stiegler, G., Kunert, R., Follenzi, A. & Goldstein, H. (2010). Inhibition of in vivo HIV infection in humanized mice by gene therapy of human hematopoietic stem cells with a lentiviral vector encoding a broadly neutralizing anti-HIV antibody. J Virol. 84, 6645-6653.
397. Kajita, M., Hogan, C., Harris, A.R., Dupre-Crochet, S., Itasaki, N., Kawakami, K., Charras, G., Tada, M. & Fujita, Y. (2010). Interaction with surrounding normal epithelial cells influences signalling pathways and behaviour of Src-transformed cells. J. Cell Sci. 123, 171-180.
396. Kazuki, Y., Hiratsuka, M., Takiguchi, M., Osaki, M., Kajitani, N., Hoshiya, H., Hiramatsu, K., Yoshino, T., Kazuki, K., Ishihara, C., Takehara, S., Higaki, K., Nakagawa, M., Takahashi, K., Yamanaka, S. & Oshimura, M. (2010). Complete genetic correction of ips cells from Duchenne muscular dystrophy. Mol. Ther. 18, 386-393.
395. Kieback, E. & Uckert, W. (2010). Enhanced T cell receptor gene therapy for cancer. Expert Opin Biol Ther. 10, 749-762.
394. Koya, R.C., Mok, S., Comin-Anduix, B., Chodon, T., Radu, C.G., Nishimura, M.I., Witte, O.N. & Ribas, A. (2010). Kinetic phases of distribution and tumor targeting by T cell receptor engineered lymphocytes inducing robust antitumor responses. Proc. Natl. Acad. Sci. USA. 107, 14286-14291.
393. Kuhns, M.S., Girvin, A.T., Klein, L.O., Chen, R., Jensen, K.D., Newell, E.W., Huppa, J.B., Lillemeier, B.F., Huse, M., Chien, Y.H., Garcia, K.C. & Davis, M.M. (2010). Evidence for a functional sidedness to the alphabetaTCR. Proc. Natl. Acad. Sci. USA 107, 5094-5099.
392. Lau, H.D., Yaegashi, J., Zaro, B.W. & Pratt, M.R. (2010). Precise control of protein concentration in living cells. Angew Chem Int Ed Engl. 49, 8458-8461.
391. Lee, S.J., Kang, S.W., Do, H.J., Han, I., Shin, D.A., Kim, J.H. & Lee, S.H. (2010). Enhancement of bone regeneration by gene delivery of BMP2/Runx2 bicistronic vector into adipose-derived stromal cells. Biomaterials 31, 5652-5659.
390. Leisegang, M., Turqueti-Neves, A., Engels, B., Blankenstein, T., Schendel, D.J., Uckert, W. & Noessner, E. (2010). T-cell receptor gene-modified T cells with shared renal cell carcinoma specificity for adoptive T-cell therapy. Clin. Cancer Res. 16, 2333-2343.
389. Leisegang, M, Wilde, S., Spranger, S., Milosevic, S., Frankenberger, B., Uckert, W. & Schendel, D.J. (2010). MHC-restricted fratricide of human lymphocytes expressing survivin-specific transgenic T cell receptors. J. Clin. Invest. 120, 3869-3877.
388. Li, H.Y., Sun, Y., Zhang, X.J., Chang, T.M., Wang, X.P., He, F., Huang, J.H. & Qiu, H.J. (2011). Generation and immunogenicity of a recombinant adenovirus co-expressing the E2 protein of Classical Swine Fever Virus and the GP5 protein of Porcine Reproduction and Respiratory Syndrome Virus. Agricultural Sciences in China 10, 1781-1791.
387. Li, M.A., Pettitt, S.J., Yusa, K. & Bradley, A. (2010). Genome-wide forward genetic screens in mouse ES cells. Methods Enzymol. 477, 217-242.
386. Li, W., Wang, D., Qin, J., Liu, C., Zhang, Q., Zhang, X., Yu, X., Lahn, B.T., Mao, F.F. & Xiang, A.P. (2010). Generation of functional hepatocytes from mouse induced pluripotent stem cells. J. Cell Physiol. 222, 492-501.
385. Lobbestael, E., Reumers, V., Ibrahimi, A., Paesen, K., Thiry, I., Gijsbers, R., Van den Haute, C., Debyser, Z., Baekelandt, V. & Taymans, J.M. (2010). Immunohistochemical detection of transgene expression in the brain using small epitope tags. BMC Biotechnol. 10, 16-26.
384. Madisen, L., Zwingman, T.A., Sunkin, S.M., Oh, S.W., Zariwala, H.A., Gu, H., Ng, L.L., Palmiter, R.D., Hawrylycz, M.J., Jones, A.R., Lein, E.S. & Zeng, H. (2010). A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat. Neurosci. 13, 133-140.
383. Maetzig, T., Galla, M., Brugman, M.H., Loew, R., Baum, C. & Schambach, A. (2010). Mechanisms controlling titer and expression of bidirectional lentiviral and gammaretroviral vectors. Gene Therapy 17, 400-411.
382. Maier P, von Kalle, C. & Laufs, S. (2010). Retroviral vectors for gene therapy. Future Microbiol. 5, 1507-1523.
381. Mak, A.B., Ni, Z., Hewel, J.A., Chen, G.I., Zhong, G., Karamboulas, K., Blakely, K., Smiley, S., Marcon, E., Roudeva, D., Li, J., Olsen, J.B., Wan, C., Punna, T., Isserlin, R., Chetyrkin, S., Gingras, A.C., Emili, A., Greenblatt, J. & Moffat, J. (2010). A lentiviral functional proteomics approach identifies chromatin remodeling complexes important for the induction of pluripotency. Mol Cell Proteomics. 9, 811-823.
380. Manicassamy, B., Manicassamy, S., Belicha-Villanueva, A., Pisanelli, G., Pulendran, B. & Garcia-Sastre, A. (2010). Analysis of in vivo dynamics of influenza virus infection in mice using a GFP reporter virus. Proc. Natl. Acad. Sci. USA. 107, 11531-11536.
379. Markley, J.C. & Sadelain, M. (2010). IL-7 and IL-21 are superior to IL-2 and IL-15 in promoting human T cell-mediated rejection of systemic lymphoma in immunodeficient mice. Blood 115, 3508-3519
378. Marton, I., Zuker, A., Shklarman, E., Zeevi, V., Tovkach, A., Roffe, S., Ovadis, M., Tzfira, T. & Vainstein, A. (2010). Non-transgenic genome modification in plant cells. Plant Physiol. 154, 1079-1087.
377. Massé, N., Davidson, A., Ferron, F., Alvarez, K., Jacobs, M., Romette, J.L., Canard, B. & Guillemot, J.C. (2010). Dengue virus replicons: production of an interserotypic chimera and cell lines from different species, and establishment of a cell-based fluorescent assay to screen inhibitors, validated by the evaluation of ribavirin's activity. Antiviral Res. 86, 296-305.
376. Mastrocola AS, Heinen CD. (2010). Lynch syndrome-associated mutations in MSH2 alter DNA repair and checkpoint response functions in vivo. Hum Mutat. 31, 1699-1708.
375. Mikkelsen, M.D., Olsen, C.E. & Halkier, B.A. (2010). Production of the cancer-preventive glucoraphanin in tobacco. Mol Plant. 3, 751-759
374. Morgan, R.A., Dudley, M.E. & Rosenberg, S.A. (2010). Adoptive cell therapy: genetic modification to redirect effector cell specificity. Cancer J. 16, 336-341.
373. Nishimura-Sakurai, Y., Sakamoto, N., Mogushi, K., Nagaie, S., Nakagawa, M., Itsui, Y., Tasaka-Fujita, M., Onuki-Karakama, Y., Suda, G., Mishima, K., Yamamoto, M., Ueyama, M., Funaoka, Y., Watanabe, T., Azuma, S., Sekine-Osajima, Y., Kakinuma, S., Tsuchiya, K., Enomoto, N., Tanaka, H. & Watanabe, M. (2010). Comparison of HCV-associated gene expression and cell signaling pathways in cells with or without HCV replicon and in replicon-cured cells. J Gastroenterol. 45, 523-536.
372. Norell, H., Zhang, Y., McCracken, J., Martins da Palma, T., Lesher, A., Liu, Y., Roszkowski, J.J., Temple, A., Callender, G.G., Clay, T., Orentas, R., Guevara-Patiño, J. & Nishimura, M.I. (2010). CD34-based enrichment of genetically engineered human T cells for clinical use results in dramatically enhanced tumor targeting. Cancer Immunol Immunother. 59, 851-862
371. Oberst, A., Pop, C., Tremblay, A.G., Blais, V., Denault, J.B., Salvesen, G.S. & Green, D.R. (2010). Inducible dimerization and inducible cleavage reveal a requirement for both processes in caspase-8 activation. J. Biol. Chem. 285, 16632-16642.
370. Okita, K., Hong, H., Takahashi. K. & Yamanaka, S. (2010). Generation of mouse-induced pluripotent stem cells with plasmid vectors. Nat. Protoc. 5, 418-428.
369. O'Leary, D.A., Vargas, L., Sharif, O., Garcia, M.E., Sigal, Y.J., Chow, S.K., Schmedt, C., Caldwell, J.S., Brinker, A. & Engels, I.H. (2010). HTS-Compatible Patient-Derived Cell-Based Assay to Identify Small Molecule Modulators of Aberrant Splicing in Myotonic Dystrophy Type 1. Curr Chem Genomics 4, 9-18.
368. Osakabe, K., Osakabe, Y. & Toki, S. (2010). Site-directed mutagenesis in Arabidopsis using custom-designed zinc finger nucleases. Proc. Natl. Acad. Sci. USA. 107, 12034-12039.
367. Osterwalder, M., Galli, A., Rosen, B., Skarnes, W.C., Zeller, R. & Lopez-Rios J. (2010). Dual RMCE for efficient re-engineering of mouse mutant alleles. Nat Methods 7, 893-895.
366. Quetglas, J.I., Ruiz-Guillen, M., Aranda, A., Casales, E., Bezunartea, J. & Smerdou, C. (2010). Alphavirus vectors for cancer therapy. Virus Res. 153, 179-196.
365. Ray, S., Chhabra, A., Chakraborty, N.G., Hegde, U., Dorsky, D.I., Chodon, T., von Euw, E., Comin-Anduix, B., Koya, R.C., Ribas, A., Economou, J.S., Rosenberg, S.A. & Mukherji, B. (2010). MHC-I-restricted melanoma antigen specific TCR-engineered human CD4+ T cells exhibit multifunctional effector and helper responses, in vitro. Clin Immunol. 136, 338-347.
364. Ribas, A. & Koya, R.C. (2010). Adoptive cell transfer of T-cell receptor-engineered lymphocytes: lessons from recent modeling. Future Oncol. 6, 1671-1673.
363. Roongsawang, N., Promdonkoy, P., Wongwanichpokhin, M., Sornlake, W., Puseenam, A., Eurwilaichitr, L. & Tanapongpipat, S. (2010). Coexpression of fungal phytase and xylanase utilizing the cis-acting hydrolase element in Pichia pastoris. FEMS Yeast Res. 10, 909-916.
362. Rothwell, D.G., Crossley, R., Bridgeman, J.S., Sheard, V., Zhang, Y., Sharp, T., Hawkins, R.E., Gilham, D. & McKay, T.R. (2010). Functional expression of secreted proteins from a bicistronic retroviral cassette based on FMDV 2A can be position-dependent. Hum. Gene Ther. 21, 1631-1637.
361. Sainsbury, F., Canizares, M.C. & Lomonossoff, G.P. (2010). Cowpea mosaic Virus: The plant virus-based biotechnology workhorse. Ann. Rev. Phytopath. 48, 437-455.
360. Sauers, D.J., Temburni, M.K., Biggins, J.B., Ceo, L.M., Galileo, D.S. & Koh, J.T. (2010). Light-activated gene expression directs segregation of co-cultured cells in vitro. ACS Chem. Biol. 5, 313-320.
359. Shalapour, S., Deiser, K., Sercan, O., Tuckermann, J., Minnich, K., Willimsky, G., Blankenstein, T., Hämmerling, G.J., Arnold, B. & Schüler, T. (2010). Commensal microflora and interferon-gamma promote steady-state interleukin-7 production in vivo. Eur. J. Immunol. 40, 2391-2400.
358. Sharma, P. (2010). Cancer immunotherapy: in vivo imaging of adoptively transferred T cells in an immunocompetent host. Proc. Natl. Acad. Sci. U.S.A. 107, 13977-13978.
357. Shao, L. & Wu, W.S. (2010). Gene-delivery systems for iPS cell generation. Expert Opin. Biol. Ther. 10, 231-242.
356. Sia, K.C., Chong, W.K., Ho, I.A., Yulyana, Y., Endaya, B., Huynh, H. & Lam, P.Y. (2010). Hybrid herpes simplex virus/Epstein-Barr virus amplicon viral vectors confer enhanced transgene expression in primary human tumors and human bone marrow-derived mesenchymal stem cells. J Gene Med. 12, 848-858.
355. Sommer, C.A., Sommer, A.G., Longmire, T.A., Christodoulou, C., Thomas, D.D., Gostissa, M., Alt, F.W., Murphy, G.J., Kotton, D.N. & Mostoslavsky, G. (2010). Excision of reprogramming transgenes improves the differentiation potential of iPS cells generated with a single excisable vector. Stem Cells 28, 64-74.
354. Stadtfeld, M., Maherali, N., Borkent, M., Hochedlinger, K.A. (2010). Reprogrammable mouse strain from gene-targeted embryonic stem cells. Nat. Methods 7, 53-55.
353. Suffner, J., Hochweller, K., Kühnle, M.C., Li, X., Kroczek, R.A., Garbi, N. &Hämmerling G.J. (2010 ). Dendritic cells support homeostatic expansion of Foxp3+ regulatory T cells in Foxp3.LuciDTR mice. J Immunol. 184, 1810-1820.
352. Tan, Y., Liang, H., Chen, A. & Guo, X. (2010). Coexpression of double or triple copies of the rabies virus glycoprotein gene using a 'self-cleaving' 2A peptide-based replication-defective human adenovirus serotype 5 vector. Biologicals 38, 586-593.
351. Tchieu, J., Kuoy, E., Chin, M.H., Trinh, H., Patterson, M., Sherman, S.P., Aimiuwu, O., Lindgren, A., Hakimian, S., Zack, J.A., Clark, A.T., Pyle, A.D., Lowry, W.E. & Plath, K. (2010). Female human iPSCs retain an inactive X chromosome. Cell Stem Cell 7, 329-342.
350. Torres, V., Barra, L., Garcés, F., Ordenes, K., Leal-Ortiz, S., Garner, C.C., Fernandez, F. & Zamorano, P. (2010). A bicistronic lentiviral vector based on the 1D/2A sequence of foot-and-mouth disease virus expresses proteins stoichiometrically. J Biotechnol. 146, 138-142.
349. Udyavar, A. & Geiger, T.L. (2010). Rebalancing immune specificity and function in cancer by T-cell receptor gene therapy. Arch. Immunol. Ther. Exp. (Warsz.) 58, 335-346.
348. Uhde-Holzem, K., Schlösser, V., Viazov, S., Fischer, R. & Commandeur, U. (2010). Immunogenic properties of chimeric potato virus X particles displaying the hepatitis C virus hypervariable region I peptide R9. J Virol Methods 166,12-20.
347. van de Sande, W.W.J., Lo-Ten-Foe, J.R., van Belkum, A., Netea, M.G., Kullberg, B.J. & Vonk, A.G. (2010). Mycoviruses: future therapeutic agents of invasive fungal infections in humans? Eur. J. Clin. Micro. Infectious Dis. 29, 755-763.
346. van Loenen, M.M., de Boer, R., Amir, A.L., Hagedoorn, R.S., Volbeda, G.L., Willemze, R., van Rood, J.J., Falkenburg, J.H. & Heemskerk, M.H. (2010). Mixed T cell receptor dimers harbor potentially harmful neoreactivity. Proc Natl Acad Sci USA. 107,10972-10977.
345. Waddington, S.N., Crossley, R., Sheard, V., Howe, S.J., Buckley, S.M.K., Coughlan, L., Gilham, D.E., Hawkins, R.E. & McKay, T.R. (2010). Gene delivery of a mutant TGFb3 reduces markers of scar tissue formation after cutaneous wounding. Mol. Ther. 18, 2104-2111
344. Wall, N.R., Wickersham, I.R., Cetin, A., De La Parra, M. & Callaway, E.M. (2010). Monosynaptic circuit tracing in vivo through Cre-dependent targeting and complementation of modified rabies virus. Proc. Natl. Acad. Sci. U.S.A. 107, 21848-21853.
343. Wang, H., Holst, J., Woo, S.R., Guy, C., Bettini, M., Wang, Y., Shafer, A., Naramura, M., Mingueneau, M., Dragone, L.L., Hayes, S.M., Malissen, Band, H. & Vignali, D.A. (2010 ). Tonic ubiquitylation controls T-cell receptor:CD3 complex expression during T-cell development. EMBO J. 29, 1285-1298.
342. Weible AP, Schwarcz L, Wickersham IR, Deblander L, Wu H, Callaway EM, Seung HS, Kentros CG. (2010). Transgenic targeting of recombinant rabies virus reveals monosynaptic connectivity of specific neurons. J. Neurosci. 30, 16509-16513.
341. Wilkie, S., Burbridge, S.E., Chiapero-Stanke, L., Pereira, A.C., Cleary, S., van der Stegen, S.J., Spicer, J.F., Davies, D.M. & Maher, J. (2010). Selective expansion of chimeric antigen receptor-targeted T-cells with potent effector function using interleukin-4. J.Biol Chem. 285, 25538-25544.
340. Wu, G., Zhang, Q.,Yan, X., Hou, J., Zhao, Z., Cui, L., Li, J. & Zhu, H. (2010). Construction of co-expressing plasmid of FMDV P1-2A gene with porcine IFN-gamma gene and expression in BHK-21 Cells. Acta Agriculturae Boreali-Sinica, 2010-05.
339. Xue, S.A., Gao, L., Thomas, S., Hart, D.P., Xue, J.Z., Gillmore, R., Voss, R.H., Morris, E. & Stauss, H.J. (2010). Development of a Wilms' tumor antigen-specific T-cell receptor for clinical trials: engineered patient's T cells can eliminate autologous leukemia blasts in NOD/SCID mice. Haematologica 95, 126-134.
338. Yan, J., Wang, H., Xu, Q., Jain, N., Toxavidis, V., Tigges, J., Yang, H., Yue, G. & Gao, W. (2010). Signal sequence is still required in genes downstream of "autocleaving" 2A peptide for secretary or membrane-anchored expression. Anal. Biochem. 399, 144-146.
337. Yang, D., Wang, C.E., Zhao, B., Li, W., Ouyang, Z., Liu, Z., Yang, H., Fan, P., O'Neill, A., Gu, W., Yi, H., Li, S., Lai, L. & Li, X.J. (2010). Expression of Huntington's disease protein results in apoptotic neurons in the brains of cloned transgenic pigs. Hum Mol Genet. 19, 3983-3994.
336. Zhang, C, (2010). DNA-based viral vector for soybean functional genomics. Plant Physiol Preview. DOI:10.1104/pp.109.151639
335. Zhang, C., Bradshaw, J.D., Whitham, S.A. & Hill, J.H. (2010). The development of an efficient multipurpose bean pod mottle virus viral vector set for foreign gene expression and RNA silencing. Plant Physiol. 153, 52-65.
334. Zhang, F., Maeder, M.L., Unger-Wallace, E., Hoshaw, J.P., Reyon, D., Christian, M., Li, X., Pierick, C.J., Dobbs, D., Peterson, T., Joung, J.K. & Voytas, D.F. (2010). High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases. Proc. Natl. Acad. Sci. USA 107, 12028-12033.
2009
333. Billeter, M.A., Naim, H.Y. & Udem, S.A. (2009). Measles: History and basic biology. Curr. Topics Microbiol. Immunol. 329, 129-162
332. Brown, C,E., Starr, R., Martinez, C., Aguilar, B., D'Apuzzo, M., Todorov, I., Shih, C.C. & Badie, B. (2009). Recognition and killing of brain tumor stem-like Initiating cells by CD8(+) cytolytic T cells. Cancer Res. 69, 8886-8893.
331. Carey, B.W., Markoulaki, S., Hanna, J., Saha, K., Gao, Q., Mitalipova, M. & Jaenisch, R. (2009). Reprogramming of murine and human somatic cells using a single polycistronic vector. Proc. Natl. Acad. Sci. USA 106, 157-162.
330. Chang, C.W., Lai, Y.S., Pawlik, K.M., Liu, K., Sun, C.W., Li, C., Schoeb, T.R. & Townes, T.M. (2009). Polycistronic lentiviral vector for "hit and run'' reprogramming of adult skin fibroblasts to induced pluripotent stem cells. Stem Cells 27, 1042-1049.
329. Chinnasamy, N., Shaffer, J. & Chinnasamy, D. (2009). Production of multicistronic HIV-1 based lentiviral vectors. Methods Mol. Biol. 515, 137-150
328. Dey, A., Kumar, U., Sharma, P. & Singh, S. (2009). Immunogenicity of candidate chimeric DNA vaccine against tuberculosis and leishmaniasis. Vaccine 7, 5152-5160.
327. Dentzer, T.G., Lorenz, I.C., Evans, M.J. & Rice, C.M. (2009). Determinants of the hepatitis C virus nonstructural protein 2 protease domain required for production of infectious virus. J Virol. 83, 12702-12713.
326. Dossett, M.L., Teague, R.M., Schmitt, T.M., Tan, X., Cooper, L.J., Pinzon, C. & Greenberg, P.D. (2009). Adoptive immunotherapy of disseminated leukemia with TCR-transduced, CD8+ T cells expressing a known endogenous TCR. Mol Ther. 17, 742-749.
325. Engert, S., Liao, W.P., Burtscher, I. & Lickert, H. (2009). Sox17-2A-iCre: a knock-in mouse line expressing Cre recombinase in endoderm and vascular endothelial cells. Genesis 47, 603-610.
324. Fragkoudis, R., Tamberg, N., Siu, R., Kiiver, K., Kohl, A. , Merits, A. & Fazakerley, J.K. (2009). Neurons and oligodendrocytes in the mouse brain differ in their ability to replicate Semliki Forest virus. J. Neurovirol. 15, 57-70.
323. Feng, L., Li, F., Liu, Y., Zheng, X., Zhang, B. & Chen, L. (2009). A convenient plasmid-based system containing three reporter genes for real-time and quantitative analysis of messenger RNA silencing. Anal Biochem. 394, 284-286.
322. Frank, R.T., Edmiston, M., Kendall, S.E., Najbauer, J., Cheung, C.W., Kassa, T., Metz, M.Z., Kim, S.U., Glackin, C.A., Wu, A.M., Yazaki, P.J. & Aboody, K.S. (2009). Neural stem cells as a novel platform for tumor-specific delivery of therapeutic antibodies. PLoS One. 4:e8314.
321. Froelich, S., Ziegler, L., Stroup, K. & Wang, P. (2009). Targeted gene delivery to CD117-expressing cells in vivo with lentiviral vectors co-displaying stem cell factor and a fusogenic molecule. Biotechnol Bioeng. 104, 206-215.
320. Gandillet, A., Serrano, A.G., Pearson, S., Lie-A-Ling, M., Lacaud, G. & Kouskoff, V. (2009). Sox7-sustained expression alters the balance between proliferation and differentiation of hematopoietic progenitors at the onset of blood specification. Blood 114, 4813-4822.
319. Geu-Flores, F., Olsen, C.E. & Halkier, B.A. (2009). Towards engineering glucosinolates into non-cruciferous plants. Planta 229, 261-270.
318. Geu-Flores, F., Nielsen, M.T., Nafis,i M., Møldrup, M.E., Olsen, C.E., Motawia, M.S. & Halkier, B.A. (2009). Glucosinolate engineering identifies a gamma-glutamyl peptidase. Nat. Chem. Biol. 5, 575-577.
317. Gonzalez, F., Monasterio, M.B., Tiscornia, G., Pulido, N.M., Vassena, R., Morera, L.B., Piza,I.R. & Belmonte, J.C.I. (2009). Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector. Proc. Natl. Acad. Sci. USA 106, 8918-8922.
316. Hanna, J., Saha, K., Pando, B., van Zon, J., Lengner, C.J., Creyghton, M.P., van Oudenaarden, A. & Jaenisch, R. (2009). Direct cell reprogramming is a stochastic process amenable to acceleration. Nature 462, 595-601.
315. Hoang-Le, D., Smeenk, L., Anraku, I., Pijlman, G.P., Wang, X.J., de Vrij, J., Liu, W.J., Le, T.T., Schroder, W.A., Khromykh, A.A. & Suhrbier, A. (2009). A Kunjin replicon vector encoding granulocyte macrophage colony-stimulating factor for intra-tumoral gene therapy. Gene Therapy 16, 190–199.
314. Hockemeyer, D., Soldner, F., Beard, C., Gao, Q., Mitalipova, M., DeKelver, R.C., Katibah, G.E., Amora, R., Boydston, E.A., Zeitler, B., Meng, X., Miller, J.C., Zhang, L., Rebar, E.J., Gregory, P.D., Urnov, F.D. & Jaenisch, R. (2009. Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Nat. Biotechnol. 27, 851-857.
313. Hohl, T.M., Rivera, A., Lipuma, L., Gallegos, A., Shi, C., Mack, M. & Pamer, E.G. (2009). Inflammatory monocytes facilitate adaptive CD4 T cell responses during respiratory fungal infection. Cell Host Microbe 6, 470-481.
312. Hu, T., Fu, Q., Chen, P., Zhang, K. & Guo, D. (2009). Generation of a stable mammalian cell line for simultaneous expression of multiple genes by using 2A peptide-based lentiviral vector. Biotechnol. Lett. 31, 353-359.
311. Hu, W.G., Jager, S., Chau, D., Mah, D. & Nagata, L.P. (2009). Generation of a recombinant full-length human antibody binding to botulinum neurotoxin A. Appl. Biochem. Biotechnol. 160, 1206-1216.
310. Ibrahimi, A., Velde, G.V., Reumers, V., Toelen, J., Thiry, I., Vandeputte, C., Vets, S., Deroose, C., Bormans, G., Baekelandt, V., Debyser, Z. & Gijsbers, R. (2009). Highly efficient multicistronic lentiviral vectors with peptide 2A sequences. Human Gene Therapy 20, 845-860.
309. Iizuka, A., Takayama, K., Torashima, T., Yamasaki, M., Koyama, C., Mitsumura, K., Watanabe, M. & Hirai, H. (2009. Lentiviral vector-mediated rescue of motor behavior in spontaneously occurring hereditary ataxic mice. Neurobiol. Dis. 35, 457-465.
308. James, S.E., Orgun, N.N., Tedder, T.F., Shlomchik, M.J., Jensen, M.C., Lin, Y., Greenberg, P.D. & Press, O.W. (2009). Antibody-mediated B-cell depletion before adoptive immunotherapy with T cells expressing CD20-specific chimeric T-cell receptors facilitates eradication of leukemia in immunocompetent mice. Blood 114, 5454-5463
307. Johnson, L.A., Morgan, R.A., Dudley, M.E., Cassard, L., Yang, J.C., Hughes, M.S., Kammula, U.S., Royal, R.E., Sherry, R.M., Wunderlich, J.R., Lee, C.C., Restifo, N.P., Schwarz, S.L., Cogdill, A.P., Bishop, R.J., Kim, H., Brewer, C.C., Rudy, S.F, VanWaes, C., Davis, J.L., Mathur, A., Ripley, R.T., Nathan, D.A., Laurencot, C.M. & Rosenberg, S.A. (2009). Gene therapy with human and mouse T-cell receptors mediates cancer regression and targets normal tissues expressing cognate antigen. Blood 114, 535-546.
306. Jones, S., Peng, P.D., Yang, S., Hsu, C., Cohen, C.J., Zhao, Y., Abad, J., Zheng, Z., Rosenberg, S.A. & Morgan, R.A. (2009). Lentiviral vector design for optimal T cell receptor gene expression in the transduction of peripheral blood lymphocytes and tumor-infiltrating lymphocytes. Human Gene Therapy 20, 630-640.
305. Kaji, K., Norrby, K., Paca, A., Mileikovsky, M., Mohseni, P. & Woltjen. K. (2009). Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Nature 458, 771-775.
304. Kang, K., Park, S., Kim, Y.S., Lee, S. & Back, K. (2009). Biosynthesis and biotechnological production of serotonin derivatives. Appl. Microbiol. Biotechnol. 83, 27-34.
303. Karner, E., Unger, C., Cerny, R., Ahrlund-Richter, L., Ganss, B., Dilber, M.S. & Wendel, M. (2009). Differentiation of human embryonic stem cells into osteogenic or hematopoietic lineages: A dose-dependent effect of osterix over-expression. J. Cellular Physiol. 218, 323-333.
302. Kitchen, S.G., Bennett, M., Galic, Z., Kim, J., Xu, Q., Young, A., Lieberman, A., Joseph, A., Goldstein, H., Ng, H., Yang, O. & Zack, J.A. (2009). Engineering antigen-specific T cells from genetically modified human hematopoietic stem cells in immunodeficient mice. PLoS One. Dec 7;4(12):e8208.
301. Kojaoghlanian, T., Joseph, A., Follenzi, A., Zheng, J.H., Leiser, M., Fleischer, N., Horwitz, M.S., DiLorenzo, T.P. & Goldstein, H. (2009). Lentivectors encoding immunosuppressive proteins genetically engineer pancreatic beta-cells to correct diabetes in allogeneic mice. Gene Ther. 16, 340-348.
300. Kunes, Y.Z., Gion, W.R., Fung, E., Salfeld, J.G., Zhu, R.R., Sakorafas, P. & Carson, G.R. (2009). Expression of antibodies using single-open reading frame vector design and polyprotein processing from mammalian cells. Biotechnol Prog. 25, 735-744.
299. Kuzmenkin, A., Liang, H., Xu, G., Pfannkuche, K., Eichhorn, H., Fatima, A., Luo, H., Saric, T., Wernig, M., Jaenisch, R. & Hescheler, J. (2009). Functional characterization of cardiomyocytes derived from murine induced pluripotent stem cells in vitro. FASEB J. 23, 4168-4180.
298. Kwong, G.A., Radu, C.G., Hwang, K., Shu, C.J., Ma, C., Koya, R.C., Comin-Anduix, B., Hadrup, S.R., Bailey, R.C., Witte, O.N., Schumacher, T.N., Ribas, A. & Heath, J.R. (2009). Modular nucleic acid assembled p/MHC microarrays for multiplexed sorting of antigen-specific T cells. J. Am. Chem. Soc. 131, 9695-9703.
297. Larochelle, A., Choi, U., Shou, Y., Naumann, N., Loktionova, N.A., Clevenger, J.R., Krouse, A., Metzger, M., Donahue, R.E., Kang, E., Stewart, C., Persons, D., Malech, H.L., Dunbar, C.E. & Sorrentino, B.P. (2009). In vivo selection of hematopoietic progenitor cells and temozolomide dose intensification in rhesus macaques through lentiviral transduction with a drug resistance gene. J. Clin. Invest. 119, 1952-1963.
296. Lennon, G.P., Bettini, M., Burton, A.R., Vincent, E., Arnold, P.Y., Santamaria, P. & Vignali, D. (2009). T cell islet accumulation in type 1 diabetes is a tightly regulated, cell-autonomous event. Immunity 31, 643-653.
295. Li, J.X., Jiang, P., Li, Y.F., Wang, X.L., Cao, J., Wang, X.W. & Zeshan, B. (2009). HSP70 fused with GP3 and GP5 of porcine reproductive and respiratory syndrome virus enhanced the immune responses and protective efficacy against virulent PRRSV challenge in pigs. Vaccine 27, 825-832.
294. Luo, X.M., Maarschalk, E., O'Connell, R.M., Wang, P., Yang, L, & Baltimore, D. (2009). Engineering human hematopoietic stem/progenitor cells to produce a broadly neutralizing anti-HIV antibody after in vitro maturation to human B lymphocytes. Blood 113, 1422-1431.
293. Matyas, G.R., Wieczorek, L., Beck, Z., Ochsenbauer-Jambor, C., Kappes, J.C., Michael, N.L., Polonis, V.R. & Alving, C.R. (2009). Neutralizing antibodies induced by liposomal HIV-1 glycoprotein 41 peptide simultaneously bind to both the 2F5 or 4E10 epitope and lipid epitopes. AIDS 23, 2069-2077.
292. Mir, F.A., Kaufmann, S.H. & Eddine, A.N. (2009). A multicistronic DNA vaccine induces significant protection against tuberculosis in mice and offers flexibility in the expressed antigen repertoire. Clin. Vaccine Immunol. 16, 1467-75.
291. Nakazawa, Y., Huye, L.E., Dotti, G., Foster, A.E., Vera, J.F., Manuri, P.R., June, C.H., Rooney, C.M. &Wilson, M.H. (2009). Optimization of the PiggyBac transposon system for the sustained genetic modification of human T lymphocytes. J. Immunother. 32, 826-836.
290. Niiya, M., Endo, M., Shang, D., Zoltick, P.W., Muvarak, N. E., Cao, W., Jin, S-Y., Skipwith, C.G., Motto, D.G., Flake, A.W. & Zheng, X.L. (2009). Correction of ADAMTS13 deficiency by in utero gene transfer of lentiviral vector encoding ADAMTS13 genes. Mol. Therapy 17, 34-41.
289. Nishiumi, F., Sone, T., Kishine, H., Thyagarajan, B., Kogure, T., Miyawaki, A., Chesnut, J.D. & Imamoto, F. (2009). Simultaneous single cell stable expression of 2-4 cDNAs in HeLaS3 using 31 integrase system. Cell Struct. Function 34, 47-59.
288. Obro, J., Borkhardt, B., Harholt, J., Skjot, M., Willats, W.G.T. & Ulvskov, P. (2009). Simultaneous in vivo truncation of pectic side chains. Transgenic Res. 18, 961-969.
287. O'Malley J, Woltjen K, Kaji K. (2009). New strategies to generate induced pluripotent stem cells. Curr. Opin. Biotechnol. 20, 516-521.
286. Pacini, L., Graziani, R., Bartholomew, L., De Francesco, R. & Paonessa, G. (2009). Naturally occurring hepatitis C virus subgenomic deletion mutants replicate efficiently in Huh-7 cells and are trans-packaged in vitro to generate infectious defective particles. J. Virol. 83, 9079-9093.
285. Papapetrou, E.P., Kovalovsky, D., Beloeil, L., Sant'angelo, D. & Sadelain, M. (2009). Harnessing endogenous miR-181a to segregate transgenic antigen receptor expression in developing versus post-thymic T cells in murine hematopoietic chimeras. J. Clin. Invest. 119, 157-168.
284. Papapetrou, E.P., Tomishima, M.J., Chambers, S.M., Mica, Y., Reed, E., Menon, J., Tabar, V., Mo, Q., Studer, L. & Sadelain, M. (2009). Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation. Proc. Natl. Acad. Sci. USA 106, 12759-12764.
283. Park, S., Kang, K., Kim, Y.S. & Back, K. (2009). Endosperm-specific expression of tyramine N-hydroxycinnamoyltransferase and tyrosine decarboxylase from a single self-processing polypeptide produces high levels of tyramine derivatives in rice seeds. Biotech. Lett. 31, 911-915.
282. Peng, P.D., Cohen, C.J., Yang, S., Hsu, C., Jones, S., Zhao, Y., Zheng, Z., Rosenberg, S.A. & Morgan, R.A. (2009). Efficient nonviral Sleeping Beauty transposon-based TCR gene transfer to peripheral blood lymphocytes confers antigen-specific antitumor reactivity. Gene Ther. 16, 1042-1049.
281. Pluta, K. & Kacprzak, M.M. (2009). Use of HIV as a gene transfer vector. Acta Biochim Pol. 56, 531-595.
280. Réjiba, S., Bigand, C., Parmentier C. & Hajri, A. (2009). Gemcitabine-based chemogene therapy for pancreatic cancer using Ad-dCK::UMK GDEPT and TS/RR siRNA strategies. Neoplasia 11, 637–650.
279. Riley, J.L. (2009). PD-1 signaling in primary T cells. Immunol. Rev. 229, 114-125.
278. Robinson, M., Li, B., Ge, Y., Ko, D., Yendluri, S., Harding, T., VanRoey, M., Spindler, K.R. & Jooss, K. (2009). A novel immune-competent murine tumor model for the evaluation of conditionally replication-competent (oncolytic) murine adenoviral vectors. J. Virol. 83, 3450-3462.
277. Rosa, A., Spagnoli, F.M. & Brivanlou, A.H. (2009). The miR-430/427/302 family controls mesendodermal fate specification via species-specific target selection. Dev Cell. 16, 517-527.
276. Sadelain M. (2009). T-cell engineering for cancer immunotherapy.Cancer J. 15, 451-455.
275. Samsa, M.M., Mondotte, J.A., Iglesias, N.G., Assunção-Miranda, .I, Barbosa-Lima, G. & Da Poian, A. (2009). Dengue virus capsid protein usurps lipid droplets for viral particle formation. PLoS Pathog. Oct;5(10):e1000632. Epub 2009 Oct 23.
274. Schmitt, T.M., Ragnarsson, G.B. & Greenberg, P.D. (2009). T Cell Receptor Gene Therapy for Cancer. Hum. Gene Ther. 20, 1240-1248.
273. Schmitz, R., Hansmann, M.L., Bohle, V., Martin-Subero, J.I., Hartmann, S., Mechtersheimer, G., Klapper, W., Vater, I., Giefing, M., Gesk, S., Stanelle, J., Siebert, R. & Küppers, R. (2009). TNFAIP3 (A20) is a tumor suppressor gene in Hodgkin lymphoma and primary mediastinal B cell lymphoma. J. Exp. Med. 206, 981-989.
272. Schrauf, S., Mandl, C.W., Bell-Sakyi, L. & Skern, T. (2009). Extension of flaviviral protein C differentially affects early RNA synthesis and growth in mammalian and arthropod host cells. J. Virol. 83, 11201-11210.
271. Shao, L., Feng, W., Sun, Y., Bai, H., Liu, J., Currie, C., Kim, J., Gama, R., Wang, Z., Qian, Z., Liaw, L. & Wu, W-S. (2009). Generation of iPS cells using defined factors linked via the self-cleaving 2A sequences in a single open reading frame. Cell Res. 19, 296-306.
270. Shi, Y., Ryabov, E.V., van Wezel, R., Li, C., Jin, M.,Wang, W., Fan, Z. & Hong, Y. (2009). Suppression of local RNA silencing is not sufficient to promote cell-to-cell movement of Turnip crinkle virus in Nicotiana benthamiana. Plant Signal Behav. 4, 15–22.
269. Shin, D., Lee, H., Kim, Yoon, Y. & Kim, M. (2009). Optimization of linear double-stranded RNA for the production of multiple siRNAs targeting hepatitis C virus. RNA 15, 898-910.
268. Sommer, C.A., Stadtfeld, M., Murphy, G.J., Hochedlinger, K., Kotton, D.N. & Mostoslavsk, G. (2009). iPS cell generation using a single lentiviral stem cell cassette. Stem Cells 27, 543-549.
267. Stewart, M.D., Jang, C.W., Hong, N.W., Austin, A.P. & Behringer, R.R. (2009). Dual fluorescent protein reporters for studying cell behaviors in vivo. Genesis 47, 708-717.
266. Suhr, S.T., Chang, E.A., Rodriguez, R.M., Wang, K., Ross, P.J., Beyhan, Z., Murthy, S. & Cibelli, J.B. (2009). Telomere dynamics in human cells reprogrammed to pluripotency. PLoS One. 4, e8124
265. Sun, Q., Choi, G.H. & Nuss, D.L. (2009). A single Argonaute gene is required for induction of RNA silencing antiviral defense and promotes viral RNA recombination. Proc. Natl. Acad. Sci. USA 106, 17927–17932.
264. Tang, W., Ehrlich, I., Wolff, S.B., Michalski, A.M., Wölfl, S., Hasan, M.T., Lüthi, A. & Sprengel, R. (2009). Faithful expression of multiple proteins via 2A-peptide self-processing: a versatile and reliable method for manipulating brain circuits. J. Neurosci. 29, 8621-8629.
263. Torashima, T., Iizuka, A., Horiuchi, H., Mitsumura, K., Yamasaki, M., Koyama, C., Takayama, K., Iino, M., Watanabe, M. & Hirai, H. (2009). Rescue of abnormal phenotypes in delta2 glutamate receptor-deficient mice by the extracellular N-terminal and intracellular C-terminal domains of the delta2 glutamate receptor. Eur J Neurosci. 30, 355-365 .
262. Thomas, J.M., Moen, S.T., Gnade, B.T., Vargas-Inchaustegui , D.A., Foltz, S.M., Suarez, G., Heidner, H.W., König, R., Chopra, A.K. & Peterson, J.W. (2009). Recombinant sindbis virus vectors designed to express protective antigen of Bacillus anthracis protect animals from anthrax and display synergy with ciprofloxacin. Clin. Vaccine Immunol. 16, 1696-1699.
261. Uckert, W. & Schumacher, T.N. (2009). TCR transgenes and transgene cassettes for TCR gene therapy: status in 2008. Cancer Immunol. Immunother 58, 809-822.
260. Ukita, K., Hirahara, S., Oshima, N., Imuta, Y., Yoshimoto, A., Jang, C.W., Oginuma, M., Saga, Y., Behringer, R.R., Kondoh, H. & Sasaki, H. (2009). Wnt signaling maintains the notochord fate for progenitor cells and supports the posterior extension of the notochord. Mech Dev. 126, 791-803.
259. von Reyn, C.R., Spaethling, J.M., Mesfin, M.N., Ma, M., Neumar, R.W., Smith, D.H., Siman, R. & Meaney, D.F. (2009). Calpain mediates proteolysis of the voltage-gated sodium channel alpha-subunit. J. Neurosci. 29, 10350-10356.
258. Wang, W., Bradley, A. & Huang, Y. (2009). A piggyBac transposon-based genome-wide library of insertionally mutated Blm-deficient murine ES cells. Genome Res. 19, 667-673.
257. Wargo, J.A, Robbins, P.F, Li, Y., Zhao, Y.B, El-Gamil, M., Caragacianu, D., Zheng, Z.L., Hong, J., Downey, S., Schrump, D., Rosenberg, S. & Morgan, R. (2009). Recognition of NY-ESO-1+tumor cells by engineered lymphocytes is enhanced by improved vector design and epigenetic modulation of tumor antigen expression. Cancer Immunol. Immunother. 58, 383-394.
256. Wilde S, Sommermeyer D, Frankenberger B, Schiemann M, Milosevic S, Spranger S, Pohla H, Uckert W, Busch DH, Schendel DJ. (2009). Dendritic cells pulsed with RNA encoding allogeneic MHC and antigen induce T cells with superior antitumor activity and higher TCR functional avidity. Blood 114, 2131-2139.
255. Woltjen, K., Michael1, I.P., Mohseni, P., Desai, R., Mileikovsky, M., Hamalainen, R., Cowling, R., Wang, W., Liu, P., Gertsenstein, M., Kaji, K., Sung,H-K. & Nagy, A. (2009). PiggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature 458, 766-770.
254. Xu, X., Zhu, D., Zhao, Q., Ao, G., Ma, C. & Yu, J. (2009). RNA silencing mediated by direct repeats in maize: A potential tool for functional genomics. Mol. Biotech. 41, 213-223.
253. Yang, Z. & Moss, B. (2009). Interaction of the vaccinia virus RNA polymerase-associated 94-kilodalton protein with the early transcription factor. J Virol. 83,12018-12026.
252. Yang, H., Joo, K.I., Ziegler, L. & Wang, P. (2009). Cell type-specific targeting with surface-engineered lentiviral vectors co-displaying OKT3 antibody and fusogenic molecule. Pharm Res. 26, 1432-1445.
251. Yoshii, K. & Holbrook, M.R. (2009). Sub-genomic replicon and virus-like particles of Omsk hemorrhagic fever virus. Virus Res. 154, 573-580.
250. Yin, Y.X., Lin, F., Zhuang, Q., Liu, L. & Qian, C. (2009). Generation of full-length functional antibody against preS2 of hepatitis B virus in hepatic cells in vitro from bicistrons mediated by gutless adenovirus. Biodrugs 23, 391-397.
249. Yun, T., Ni, Z., Yu, B., Chen, L., Hua, J., Wang, G. & Liu, G. (2009). Construction and immunogenicity of recombinant adenovirus co-expressing the GP5 and M protein of porcine reproduction and respriratory syndrome virus in mice. Sheng Wu Gong Cheng Xue Bao 25, 488-495. (Article in Chinese).
248. Yusa, K., Rad, R., Takeda, J. & Bradley, A. (2009). Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon. Nat Methods. 6, 363-369.
247. Zheng, M., Jin, N., Liu, Q., Huo, X., Li, Y., Hua, B., Ma, H., Zhu, Z., Cong, Y., Li, X., Jin, M. & Zhu, G. (2009). Immunogenicity and protective efficacy of Semliki forest virus replicon-based DNA vaccines encoding goatpox virus structural proteins. Virology 391, 33–43.
246. Zhao, B., Song, A., Haque, R., Lei, F., Weiler, L., Xiong, X., Wu, Y., Croft, M. & Song, J. (2009). Cooperation between molecular targets of costimulation in promoting T cell persistence and tumor regression. J. Immunol. 182, 6744-6752.
245. Zhou, Z., Callaway, K.A., Weber, D.A. & Jensen, P.E. (2009). Cutting edge: HLA-DM functions through a mechanism that does not require specific conserved hydrogen bonds in class II MHC-peptide complexes. J. Immunol. 183, 4187- 4191.
2008
244. Alli, R., Nguyen, P. & Geiger, T.L. (2008). Retrogenic modeling of experimental allergic encephalomyelitis associates T cell frequency but not TCR functional affinity with pathogenicity. J. Immunol. 181, 136-145.
243. Anrakua, I., Mokhonovb, V.V., Rattanasenaa, P., Mokhonovab, E.I., Leungb, J., Pijlmanb, G., Carac, A., Schrodera, W.A., Khromykhb, A.A. & Suhrbiera, A. (2008). Kunjin replicon-based simian immunodeficiency virus gag vaccines. Vaccine 26, 3268-3276.
242. Basu, S., Golovina, T., Mikheeva, T., June, C.H. & Riley, J.L. (2008). Foxp3-mediated induction of Pim 2 allows human T regulatory cells to preferentially expand in rapamycin. J. Immunol. 180, 5794-5798.
241. Burton, A.R., Vincent, E., Arnold, P.Y., Lennon, G.P., Smeltzer, M., Li, C.S., Haskins, K., Hutton, J., Tisch, R.M., Sercarz, E.E., Santamaria, P., Workman, C.J. & Vignali, D.A. (2008). On the pathogenicity of autoantigen-specific T-cell receptors. Diabetes 57, 1321-1330.
240. Chaparro, R.J., Burton, A.R., Serreze, D.V., Vignali, D.A. & DiLorenzo, T.P. (2008). Rapid identification of MHC class I-restricted antigens relevant to autoimmune diabetes using retrogenic T cells. J. Immunol. Methods 335, 106-115.
239. Chhabra, A., Yang, L., Wang, P., Comin-Anduix, B., Das, R., Chakraborty, N.G., Ray, S., Mehrotra, S., Yang, H., Hardee, C.L., Hollis, R., Dorsky, D.I., Koya, R., Kohn, D.B., Ribas, A., Economou, J.S., Baltimore, D. & Mukherji, B. (2008). CD4+ CD25- T Cells transduced to express MHC class I-restricted epitope-specific TCR synthesize Th1 cytokines and exhibit MHC class I-restricted cytolytic effector function in a human melanoma model. J. Immunol. 181, 1063-1070
238. d'Apice, A.J. & Cowan, P.J. (2008). Gene-modified pigs. Xenotransplantation. 15, 87-90.
237. Fan, Z.C. & Bird, R.C. (2008). Generation and characterization of an N-pro-disrupted marker bovine viral diarrhea virus derived from a BAC cDNA. J. Virol. Methods 151, 257-263.
236. Fan, Z.C, Dennis, J.C. & Bird, R.C. (2008). Bovine viral diarrhea virus is a suitable viral vector for stable expression of heterologous gene when inserted in between N-pro and C genes. Virus Res. 138, 97-104.
235. Gajavelli, S., Castellanos, D.A,, Furmanski, O., Schiller, P.C. & Sagen, J. (2008). Sustained analgesic peptide secretion and cell labeling using a novel genetic modification. Cell Transplant. 17, 445-455.
234. Griffioen, M., van Egmond, H.M., Barnby-Porritt, H., van der Hoorn, M.A., Hagedoorn, R.S., Kester, M.G., Schwabe, N., Willemze, R., Falkenburg, J.H. & Heemskerk, M.H. (2008). Genetic engineering of virus-specific T cells with T-cell receptors recognizing minor histocompatibility antigens for clinical application. Haematologica 93, 1535-1543.
233. Hart, D.P., Xue, S-A., Thomas, S., Cesco-Gaspere, M., Tranter, M., Willcox, B., Lee, S.P., Steven, N., Morris, E.C. & Stauss, H.J. (2008). Retroviral transfer of a dominant TCR prevents surface expression of a large proportion of the endogenous TCR repertoire in human T cells. Gene Therapy 15, 625-631.
232. Herold, M.J., van den Brandt, J., Seibler, J. & Reichardt, H.M. (2008). Inducible and reversible gene silencing by stable integration of an shRNA-encoding lentivirus in transgenic rats. Proc. Natl. Acad. Sci. USA 105, 18507–18512.
231. Hickerson, R.P., Smith, F.J.D., Reeves, R.E., Contag, C.H., Leake, D., Leachman, S.A., Milstone, L.M., Leonard, M., McLean, W.H.I. & Kaspar, R.L. (2008). Single-nucleotide-specific siRNA targeting in a dominant-negative skin model. J. Invest. Dermatol. 128, 594-605.
230. Hsiao, E.C., Yoshinaga, Y., Nguyen, T.D., Musone, S.L., Kim, J.E., Swinton, P., Espineda, I., Manalac, C., deJong, P.J. & Conklin, B.R. (2008). Marking embryonic stem cells with a 2A self-cleaving peptide: A NKX2-5 emerald GFP BAC reporter. PLoS ONE. 3(7): e2532.
229. Holst, J., Wang, H., Eder, K.D, Workman, C.J., Boyd, K.L., Baquet, Z., Singh, H., Forbes, K., Chruscinski, A., Smeyne, R., van Oers, N.S.C., Utz, P.J. & Vignali, D.A.A. (2008). Scalable signaling mediated by T cell antigen receptor– CD3 ITAMs ensures effective negative selection and prevents autoimmunity. Nature Immunol. 9, 658-666.
228. Jost, S., Turelli, P., Mangeat, B., Protzer, U. & Trono, D. (2007). Induction of antiviral cytidine deaminases does not explain the inhibition of hepatitis B virus replication by interferons. J. Virol. 81, 10588-10596.
227. Kang, S.-J., Liang, H.-E., Reizis, B., & Locksley, R.M. (2008). Regulation of hierarchical clustering and activation of innate immune cells by dendritic cells. Immunity 29, 819-833.
226. Lee, Z.H., Dennis, J.E. & Gerson, S.L. (2008). Imaging stem cell implant for cellular-based therapies. Exp. Biol. Med. 233, 930-940.
225. Leisegang, M., Engels, B., Meyerhuber, P., Kieback, E., Sommermeyer, D., Xue, S-A., Reuß, S., Stauss, H. & Uckert, W. (2008). Enhanced functionality of T cell receptor-redirected T cells is defined by the transgene cassette. J. Mol. Med. 86, 573-583.
224. Li,J., Jiang, P., Li, Y. Wang, X., Cao, J., Wang, X. & Zeshan, B. (2008). HSP70 fused with GP3 and GP5 of porcine reproductive and respiratory syndrome virus enhanced the immune responses and protective efficacy against virulent PRRSV challenge in pigs. Vaccine 27, 825-832.
223. Lico, C., Chen, Q. & Santi, L. (2008). Viral vectors for production of recombinant proteins in plants. J. Cell. Physiol. 216, 366-377.
222. Liston, A., Nutsch, K.M., Farr, A.G., Lund, J.M., Rasmussen, J.P., Koni, P.A. & Rudensky, A.Y. (2008). Differentiation of regulatory Foxp3+ T cells in the thymic cortex. Proc Natl Acad Sci USA. 105, 11903-11908.
221. Ma, M.X., Jin, N., Shen, G., Zhu, G., Liu, H.J., Zheng, M., Lu, H., Huo, X., Jin, M., Yin, G., Ma, H., Li, X., Ji, Y. & Jin, K. (2008). Immune responses of swine inoculated with a recombinant fowlpox virus co-expressing P12A and 3C of FMDV and swine IL-18. Vet Immunol. Immunopathol. 121, 1-7.
220. Miller, J.C., Holmes, M.C., Wang, J., Guschin, D.Y., Lee, Y.L., Rupniewski, I., Beausejour, C.M., Waite, A.J., Wang, N.S., Kim, K.A., Gregory, P.D., Pabo, C.O. & Rebar, E.J. (2007). An improved zinc-finger nuclease architecture for highly specific genome editing. Nature Biotech. 25, 778-785.
219. Moisini, I., Nguyen, P., Fugger, L. & Geiger. T.L. (2008). Redirecting therapeutic T cells against myelin-specific T lymphocytes using a humanized myelin basic protein-HLA-DR2-zeta chimeric receptor. J. Immunol. 180, 3601-3611.
218. Mulloy, J.C., Wunderlich, M., Zheng, Y., Wei, J.P. (2008). Transforming human blood stem and progenitor cells: A new way forward in leukemia modeling. Cell Cycle 7, 3314-3319.
217. O'Connell-Rodwell, C.E., Mackanos, M.A., Simanovskii, D., Cao, Y.A., Bachmann, M.H., Schwettman, H.A. & Contag, C.H. (2008). In vivo analysis of heat-shock-protein-70 induction following pulsed laser irradiation in a transgenic reporter mouse. J. Biomed. Optics 13, Article # 030501.
216. Okita, K., Nakagawa, M., Hyenjong,H., Ichisaka, T. & Yamanaka, S. (2008). Generation of mouse induced pluripotent stem cells without viral vectors. Science 322, 949-953.
215. Park, M., Kang, K., Park, S., Kim, Y.S., Ha, S.H., Lee, S.W., Ahn, M.J., Bae, J.M. & Back, K. (2008). Expression of serotonin derivative synthetic genes on a single self-processing polypeptide and the production of serotonin derivatives in microbes. App. Microbiol. Biotech. 81, 43-49.
214. Pfuetzner, W. (2008). Retroviral bicistronic vectors. Drug News & Perspectives 21, 473-480
213. Richardson, M.W., Carroll, R.G., Stremlau, M., Korokhov, N., Humeau, L.M., Silvestri, G., Sodroski, J., & Riley, J.L. (2008). Mode of transmission affects the sensitivity of human immunodeficiency virus type 1 to restriction by rhesus TRIM5alpha. J. Virol. 82, 1117–11128.
212. Robbins, P.F., Li, Y.F., El-Gamil, M., Zhao, Y., Wargo, J.A., Zheng, Z., Xu, H., Morgan, R.A., Feldman, S.A., Johnson, L.A., Bennett, A.D., Dunn, S.M., Mahon, T.M., Jakobsen, B.K. & Rosenberg, S.A. (2008). Single and dual amino acid substitutions in TCR CDRs can enhance antigen-specific T cell functions. J. Immunol. 180, 6116-6131.
211. Rosenberg, S.A., Restifo, N.P., Yang, J.C., Morgan, R.A. & Dudley, M.E. (2008). Adoptive cell transfer: a clinical path to effective cancer immunotherapy. Nat Rev Cancer 8, 299-308.
210. Sabatino, J.J., Shires, J., Altman, J.D., Ford, M.L. & Evavold, B.D. (2008). Loss of IFN-gamma enables the expansion of autoreactive CD4(+) T cells to induce experimental autoimmune encephalomyelitis by a nonencephalitogenic myelin variant antigen. J. Immunol. 180, 4451-4457.
209. Samalova, M., Fricker, M. & Moore, I. (2008). Quantitative and qualitative analysis of plant membrane traffic using fluorescent proteins. Meth. Cell Biol. 85, 353-380.
208. Simmons, A.D., Moskalenko, M., Creson, J,. Fang, J.M., Yi, S.L., VanRoey, M.J., Allison, J.P. & Jooss, K. (2008). Local secretion of anti-CTLA-4 enhances the therapeutic efficacy of a cancer immunotherapy with reduced evidence of systemic autoimmunity. Cancer Immunol. Immunother. 57, 1263-1270.
207. Stern, P., Astrof, S., Erkeland, S.J., Schustak, J., Sharp, P.A. & Hynes, R.O. (2008). A system for cre-regulated RNA interference in vivo. Proc. Natl. Acad. Sci. USA 105, 13895-13900.
206. Trichas, G., Begbie, J. & Srinivas, S. (2008). Use of the viral 2A peptide for bicistronic expression in transgenic mice. BMC Biology 6, Article #40.
205. Ueno, Y. (2008). Functionalization of viral protein assemblies by self-assembly reactions. J. Materials Chem. 18, 3741-3745.
204. Unger, C., Kärner, E., Treschow, A., Stellan, B., Felldin, U., Concha, H., Wendel, M., Hovatta, O., Aints, A., Ahrlund-Richter, L. & Dilber, M.S. (2008). Lentiviral-mediated HoxB4 expression in human embryonic stem cells initiates early hematopoiesis in a dose-dependent manner but does not promote myeloid differentiation. Stem Cells 26, 2455-2466.
203. Varela-Rohena, A., Carpenito, C., Perez, E. E., Richardson, M., Parry, R.V., Milone, M., Scholler, J., Hao, X., Mexas, A., Carroll, R.G.. June, C.H. & Riley, J.L. (2008). Genetic engineering of T cells for adoptive immunotherapy. Immunologic Res. 42, 166-181.
202. Wei, J., Wunderlich, M., Fox, C., Alvarez, S., Cigudosa, J.C., Wilhelm, J.S., Zheng, Y., Cancelas, J.A., Gu, Y., Jansen, M., Dimartino, J.F. & Mulloy, J.C. (2008). Microenvironment determines lineage fate in a human model of MLL-AF9 leukemia. Cancer Cell 13, 483–495.
201. Yang, S., Cohen, C.J., Peng, P.D., Zhao, Y., Cassard, L., Yu, Z., Zheng, Z., Jones, S., Restifo, N.P., Rosenberg, S.A. & Morgan, R.A. (2008). Development of optimal bicistronic lentiviral vectors facilitates high-level TCR gene expression and robust tumor cell recognition. Gene Therapy 15, 1411-1423.
200. Yang, H., Ziegler, L., Joo, K-I., Cho, T., Lei, Y. & Wang, P. (2008). Gamma-retroviral vectors enveloped with an antibody and an engineered fusogenic protein achieved antigen-specific targeting. Biotechnol. Bioeng. 101, 357-368.
199. Yun, T., Ni, Z., Yu, B., Chen, L., Hua, J., Wang, G. & Liu, G. (2009). Construction and immunogenicity of recombinant adenovirus co-expressing the GP5 and M protein of porcine reproduction and respriratory syndrome virus in mice. Sheng Wu Gong Cheng Xue Bao. 25, 488-495. (Article in Chinese)
198. Zhang, X & Nuss, D.L. (2009).A host dicer is required for defective viral RNA production and recombinant virus vector RNA instability for a positive sense RNA virus. Proc. Natl. Acad. Sci. USA. 105, 16749–16754.
197. Zhang, Y.J., Weady, P., Duggal, R., & Hao, W. (2008). Novel chimeric genotype 1b/2a hepatitis C virus suitable for high-throughput screening. Antimicrobial Agents Chemother. 52, 666-674.
2007
196. Bontkes, H.J., Kramer, D., Ruizendaal, J.J., Kueter, E.W.M., van Tendeloo, V.F.I., Meijer, C.J.L.M. & Hooijberg, E. (2007). Dendritic cells transfected with interleukin-12 and tumor-associated antigen messenger RNA induce high avidity cytotoxic T cells. Gene Therapy 14, 366-375.
195. Carette, N., Engelkamp, H., Akpa, E., Pierre, S.J., Cameron, N.R., Christianen, P.C.M., Maan, J.C., Thies, J.C., Weberskirch, R., Rowan, A.E., Nolte, R.J.M., Michon, T. & Van Hest, J.C.M. (2007). A virus-based biocatalyst. Nature Nanotechnol. 2, 226 - 229.
194. Chang, C.L., Tsai, Y.C., He, L., Wu, T.C. & Hung, C.F. (2007). Cancer immunotherapy using irradiated tumor cells secreting heat shock protein 70. Cancer Res. 67, 10047-10057.
193. Collison, L.W., Workman, C.J., Kuo, T.T., Boyd, K., Wang, Y., Vignali, K.M., Cross, R., Sehy, D., Blumberg, R.S. & Vignali, D.A. (2007). The inhibitory cytokine IL-35 contributes to regulatory T-cell function. Nature 450, 566-569.
192. Contag, C.H. (2007). In vivo pathology: Seeing with molecular specificity and cellular resolution in the living body Ann. Rev. Pathology-Mechansims of Dis. 2, 277-305.
191. Deroose, C., Gijsbers, R., Vande Velde, G., Vandeputte, C., Reumers, V., Thiry, I., Baekelandt, V., Mortelmans, L. & Debyser, Z. (2007). Systematic evaluation of bicistronic lentiviral vector constructs for reporter gene imaging. J. Nucl Med. 48 (Supplement 2):245P
190. Dobbs, A.K., Yang, T., Farmer, D., Kager, L., Parolini, O. & Conley, M.E. (2007). Cutting edge: a hypomorphic mutation in Igbeta (CD79b) in a patient with immunodeficiency and a leaky defect in B cell development. J. Immunol. 179, 2055-2059.
189. Dumas, E., Masante, C., Astier-Gin, T., Lapaillerie, D. & Ventura, M. (2007). The hepatitis C virus minigenome: A new cellular model for studying viral replication. J. Virol. Meth. 142, 59-66.
188. Engels, B. & Uckert, W. (2007). Redirecting T lymphocyte specificity by T cell receptor gene transfer—a new era for immunotherapy. Mol Aspects Med. 28, 115–142.
187. Fang, J.M., Yi, S.L., Simmons, A., Tu, G.H., Nguyen, M., Harding, T.C., VanRoey, M. & Jooss, K. (2007). An antibody delivery system for regulated expression of therapeutic levels of monoclonal antibodies in vivo. Mol. Therapy 15, 1153-1159.
186. Folimonov, A.S., Folimonova, S.Y., Bar-Joseph, M. & Dawson, W.O. (2007). A stable RNA virus-based vector for citrus trees. Virology 368, 205-216.
185. Hasegawa, K., Cowan, A.B., Nakatsuji, N. & Suemori, H. (2007). Efficient multicistronic expression of a transgene in human embryonic stem cells. Stem Cells 25, 1707-1712.
184. Hao, W.D., Herlihy, K.J., Zhang, N.J., Fuhrman, S.A., Doan, C., Patick, A.K. & Duggal, R. (2007). Development of a novel dicistronic reporter-selectable hepatitis C virus replicon suitable for high-throughput inhibitor screening. Antimicrob. Agents & Chemotherapy 51, 95-102.
183. Hong,Q., Qian,P., Li, X-M., Yu, X-L. & Chen, H-C. (2007). A recombinant pseudorabies virus co-expressing capsid proteins precursor P1-2A of FMDV and VP2 protein of porcine parvovirus: a trivalent vaccine candidate. Biotech. Lett. 29, 1677-1683.
182. Ito-Harashima, S., Kuroha, K., Tatematsu, T. & Inada, T. (2007). Translation of the poly(A) tail plays crucial roles in nonstop mRNA surveillance via translation repression and protein destabilization by proteasome in yeast. Genes & Dev. 21, 519-524.
181. Jost, S., Turelli, P., Mangeat, B., Protzer, U. & Trono, D. (2007). Induction of antiviral cytidine deaminases does not explain the inhibition of hepatitis B virus replication by interferons. J. Virol. 81, 10588-10596.
180. Kelly, B.J., Fleeton, M.N. & Atkins, G.J. (2007). Potential of alphavirus vectors in the treatment of advanced solid tumors. Recent Patents Anti-Cancer Drug Disc. 2, 159-166.
179. Kuhns, M.S. & Davis, M.M. (2007). Disruption of extracellular interactions impairs T cell receptor-CD3 complex stability and signaling. Immunity 26, 357-369.
178. Liu, B.S., Liu, X.Y. & Qian, C. (2007). An efficient tool for the construction of multiple-cistronic vectors: FMDV 2A. Sheng Wu Gong Cheng Xue Bao 23, 765-9. [Article in Chinese]
177. Lyons, J.A., Sheahan, B.J., Galbraith, S.E., Mehra, R., Atkins, G.J. & Fleeton, M.N. (2007). Inhibition of angiogenesis by a Semliki Forest virus vector expressing VEGFR-2 reduces tumour growth and metastasis in mice. Gene Therapy 14, 503-513.
176. Miloud, T., Henrich, C. & Hämmerling, G.J. (2007). Quantitative comparison of click beetle and firefly luciferases for in vivo bioluminescence imaging. J. Biomed. Opt. 12, 054018.
175. Provost, E., Rhee, J. & Leach, S.D. (2007). Viral 2A peptides allow expression of multiple proteins from a single ORF in transgenic zebrafish embryos. Genesis 45, 625-629.
174. Quintarelli, C., Vera, J.F., Savoldo, B., Attianese, G.M.P.G., Pule, M., Foster, A.E., Heslop, H.E., Rooney, C.M., Brenner, M.K. & Dotti, G. (2007). Co-expression of cytokine and suicide genes to enhance the activity and safety of tumor-specific cytotoxic T lymphocytes. Blood 110, 2793-2802.
173. Santi, L., Huang, Z. & Mason, H. (2006). Virus-like particles production in green plants. Methods 40, 66-76.
172. Shustov, A.V., Mason, P.W & Frolov, I. (2007). Production of pseudoinfectious Yellow Fever Virus with a two-component genome. J. Virol. 81, 11737–11748.
171. Stephan, M.T., Ponomarev, V., Brentjens, R.J., Chang, A.H., Dobrenkov, K.V., Heller, G. & Sadelain, M. (2007). T cell-encoded CD80 and 4-1BBL induce auto- and transcostimulation, resulting in potent tumor rejection. Nature Med. 13, 14401449.
170. Teh, O-K & Moore, I. (2007). An ARF-GEF acting at the Golgi and in selective endocytosis in polarized plant cells. Nature 448, 493-497.
169. Thomas, S., Hart, D.P., Xue, S.A., Cesco-Gaspere, M. & Stauss, H.J. (2007). T-cell receptor gene therapy for cancer: the progress to date and future objectives. Exp. Op. Biol. Ther. 7, 1207-1218.
168. van den Born, E., Posthuma, C.C., Knoops, K. & Snijder, E.J. (2007). An infectious recombinant equine arteritis virus expressing green fluorescent protein from its replicase gene. J. Gen. Virol. 88, 1196-1205.
167. Wang, Q., Ilves, H., Chu, P., Contag, C.H., Leake, D., Johnston, B.H. & Kaspar, R.L. (2007). Delivery and inhibition of reporter genes by small interfering RNAs in a mouse skin model. J. Invest. Dermatology 127, 2577-2584.
166. Wang, S., Yao, Q., Tao, J., Qiao, Y. & Zhang, Z. (2007). Co-ordinate expression of glycine betaine synthesis genes linked by the FMDV 2A region in a single open reading frame in Pichia pastoris. App. Micro. & Biotech. 77, 891-899.
2006
165. Cañizares, M.C., Liu, L., Perrin, Y., Tsakiris, E & Lomonossoff, G.P. (2006). A bipartite system for the constitutive and inducible expression of high levels of foreign proteins in plants. Plant Biotech. J. 4,183–193.
164. Chinnasamy, D., Milsom, M.D., Shaffer, J., Neuenfeldt, J., Shaaban, A.F., Margison, G.P., Fairbairn, L.J. & Chinnasamy, N. (2006). Multicistronic lentiviral vectors containing the FMDV 2A cleavage factor demonstrate robust expression of encoded genes at limiting MOI. Virology J. 3, 14.
163. Chikkanna-Gowda, C.P., McNally,S., Sheahan, B.J., Fleeton, M.N. & Atkins, G.J. (2006). Inhibition of urine K-BALB and CT26 tumour growth using a Semliki Forest virus vector with enhanced expression of IL-18. Oncology Rep.16, 713-719.
162. de Felipe, P., Luke, G.A., Hughes, L.E., Gani, D., Halpin, C. & Ryan, M.D. (2006). E unum pluribus: multiple proteins from a self-processing polyprotein. Trends in Biotech. 24, 68-75.
161. Fricker,M., Runions, J. & Moore, I. (2006). Quantitative fluorescence microscopy: for art to science. Ann. Rev. Plant Biol. 57, 79-107.
160. Friel, J., Schiedlmeier, B., Geldmacher, M. & Ostertag, W. (2006). Stromal cells selectively reduce the growth advantage of human committed CD34+ hematopoietic cells ectopically expressing HOXB4. Growth Factors 24, 97-105.
159. Graham, A., Walker, R., Baird, P., Hahn, C.N. & Fazakerley, J.K. (2006). CNS Gene Therapy Applications of the Semliki Forest Virus 1 Vector are Limited by Neurotoxicity. Molecular Therapy 13, 631–635.
158. Holden, K.L., Stein, D.A., Pierson, T.C., Ahmed, A.A., Clyde, K., Iversen, P.L. & Harris, E. (2006). Inhibition of dengue virus translation and RNA synthesis by a morpholino oligomer targeted to the top of the terminal 3 ' stem-loop structure. Virology 344, 439-452.
157. Holst, J., Szymczak-Workman, A.L., , K.M., Burton, A.R., Workman, C.J. & Vignali, D.A.A. (2006). Generation of T-cell receptor retrogenic mice. Nature Protocols 1, 406-417.
156. Holst, J., Vignali, K.M., Burton, A.R. & Vignali, D.A.A. (2006). Rapid analysis of T-cell selection in vivo using T cell-receptor retrogenic mice. Nature Methods 3, 191-197.
155. Huang, X., Wilber, A.C., Bao, L., Tuong, D., Tolar, J., Orchard, P.J., Levine, B.L., June, C.H., McIvor, R.S., Blazar, B.R., & Zhou, X.Z. (2006). Stable gene transfer and expression in human primary T cells by the Sleeping Beauty transposon system. Blood 107, 483-491.
154. Johnson, L.A., Heemskerk, B., Powell, D.J. Jr., Cohen, C.J., Morgan, R.A., Dudley, M.E., Robbins, P.F. & Rosenberg, S.A. (2006). Gene transfer of tumor-reactive TCR confers both high avidity and tumor reactivity to nonreactive peripheral blood mononuclear cells and tumor-infiltrating lymphocytes. J Immunol. 177, 6548-6559.
153. Kim, M., Shin, D., Kim, S.I. & Park, M. (2006). Inhibition of hepatitis C virus gene expression by small interfering RNAs using a tri-cistronic full-length viral replicon and a transient mouse model. Virus Res. 122, 1-10.
152. Marconi, G., Albertini, E., Barone, P., De Marchis, F., Lico, C., Marusic, C., Rutili, D., Veronesi, F. & Porceddu, A. (2006). In planta production of two peptides of the Classical Swine Fever Virus (CSFV) E2 glycoprotein fused to the coat protein of potato virus X. BMC Biotechnology 6, 29.
151. Monger, W., Alamillo, J.M., Sola, I., Perrin, Y., Bestagno, M., Burrone, O.R., Sabella, P., Plana-Duran, J., Enjuanes, L., Garcia, J.A. & Lomonossoff, G.P. (2006). An antibody derivative expressed from viral vectors passively immunizes pigs against transmissible gastroenteritis virus infection when supplied orally in crude plant extracts. Plant Biotech. J. 4, 623–631.
150. Park, J.H., Kim, S.J., Oem, J.K., Lee, K.N., Kim, Y.J., Kye, S.J., Park,J.Y. and Joo, Y.S. (2006). Enhanced immune response with foot and mouth disease virus VP1 and interleukin-1 fusion genes. J. Vet. Sci., 7, 257–262.
149. Pijlman, G., Suhrbier, A & Khromykh, A.A. (2006). Kunjin virus replicons; an RNA-based, non-cytopathic viral vector system for protein production, vaccine and gene therapy applications. Expert Opin. Biol. Ther. 6, 135-145.
148. Premraj, A., Sreekumar, E., Jain, M. & Rasool, T.J. (2006). Buffalo (Bubalus bubalis) interleukin-12: Analysis of expression profiles and functional cross-reactivity with bovine system. Mol. Immunol. 43, 822-829.
147. Puig-Basagoiti, F., Tilgner, M., Forshey, B.M., Philpott, S.M., Espina, N.G., Wentworth, D.E., Goebel, S.J., Masters, P.S., Falgout, B., Ren, P., Ferguson, D.M. & Shi, P.Y. (2006) Triaryl pyrazoline compound inhibits flavivirus RNA replication. Antimicrob. Agents Chemother. 50, 1320-1329.
146. Roan, N.R. & Starnbach, M.N. (2006). Antigen-specific CD8+ T cells respond to Chlamydia trachomatis in the genital mucosa. J. Immunol. 177, 7974-7979.
145. Samalova, M., Fricker, M. & Moore, I. (2006). Ratiometric fluorescence-imaging assays of plant membrane traffic using polyproteins. Traffic 7, 1701-1723.
144. Santi, L., Huang, Z. & Mason, H. (2006). Virus-like particles production in green plants. Methods 40, 66-76.
143. Scholten, K.B., Kramer, D., Kueter, E.W., Graf, M., Schoedl, T., Meijer, C.J., Schreurs, M.W. & Hooijberg, E. (2006). Codon modification of T cell receptors allows enhanced functional expression in transgenic human T cells. Clin. Immunol. 119, 135-145.
142. Southgate, T.D., Garside, E., Margison, G.P. & Fairbairn, L.J. (2006). Dual agent chemoprotection by retroviral co-expression of either MDR1 or MRP1 with the P140K mutant of O6-methylguanine-DNA-methyl transferase. J. Gene Med. 8, 972-979.
141. Sun, J.C., Lehar, S.M. & Bevan, M.J. (2006). Augmented IL-7 signaling during viral infection drives greater expansion of effector T cells but does not enhance memory. J. Immunol. 177, 4458-4463.
140. Will, E., Speidel, D., Wang, Z., Ghiaur, G., Rimek, A., Schiedlmeier, B., Williams, D.A., Baum, C., Ostertag, W. & Klump, H. (2006). HOXB4 inhibits cell growth in a dose-dependent manner and sensitizes cells towards extrinsic cues. Cell Cycle 5, 14-22.
139. Wimmer, V.C., Horstmann, H., Groh, A. & Kuner, T. (2006). Donut-like topology of synaptic vesicles with a central cluster of mitochondria wrapped into membrane protrusions: a novel structure-function module of the adult calyx of Held. J. Neurosci. 26, 109-16.
138. Woolford, L.B., Southgate, T.D, Margison, G.P., Milsom, M.D. & Fairbairn, L.J. (2006). The P140K mutant of human O-6-methylguanine-DNA-methyttransferase (MGMT) confers resistance in vitro and in vivo to temozolomide in combination with the novel MGMT inactivator O6-(4-bromothenyl)guanine. J. Gene Med. 8, 29-34.
137. Wozniak, T.M., Ryan, A.A., Triccas, J.A. &. Britton, W.J. (2006). Plasmid interleukin-23 (IL-23), but not plasmid IL-27, enhances the protective efficacy of a DNA vaccine against Mycobacterium tuberculosis Infection. Infec. Immunity, 74, 557-565
136. Wozniak, T.M., Ryan, A.A., Britton, W.J. (2006). Interleukin-23 restores immunity to Mycobacterium tuberculosis infection in IL-12p40-deficient mice and is not required for the development of IL-17-secreting T cell responses. J. Immunol. 177, 8684-8692.
135. Xiaowu, P., Fu, W.C., Guo, Y.H., Zhang, L.S., Xie, T.P. & Xinbin, G. (2006). Construction of recombinant yellow fever virus 17D containing 2A fragment as a vaccine vector. Sheng Wu Gong Cheng Xue Bao. 22, 492-498. [Article in Chinese].
134. Yusibov, V., Rabindran, S., Commandeur, U., Twyman, R. M. & Fischer, R. (2006). The potential of plant virus vectors for vaccine production. Drugs in R & D. 7, 203-217.
133. Zelada, A.M., Calamante, G., de la Paz Santangelo, M., Bigi,F., Verna, F., Mentaberry, A. & Cataldi, Á. (2006). Expression of tuberculosis antigen ESAT-6 in Nicotiana tabacum using a potato virus X-based vector. Tuberculosis 86, 263–267.
2005
132. Afonso, E.D.S., Escriou, N., Leclercq, I., van der Werf, S. & Naffakh, N. (2005). The generation of recombinant influenza A viruses expressing a PB2 fusion protein requires the conservation of a packaging signal overlapping the coding and noncoding regions at the 5 ' end of the PB2 segment. Virology 341, 34-46.
131. Alvarez, D.E., Ezcurra, A.L.D., Fucito, S. & Gamarnik, A.V. (2005). Role of RNA structures present at the 3 ' UTR of dengue virus on translation, RNA synthesis, and viral replication. Virology 339, 200-212.
130. Amendola, M., Venneri, M.A., Biffi, A., Vigna, E. & Naldini, L. (2005). Coordinate dual-gene transgenesis by lentiviral vectors carrying synthetic bidirectional promoters. Nature Biotech. 23, 108-16.
129. Cañizares, M.C., Nicholson, L. & Lomonossoff, G.P. (2005). Use of viral vectors for vaccine production in plants. Immunol. Cell Biol. 83, 263.
128. Cao, Y.A., Bachmann, M.H., Beilhack, A., Yang, Y., Tanaka, M., Swijnenburg, R.J., Reeves, R., Taylor-Edwards, C., Schulz, S., Doyle, T.C., Fathman, C.G., Robbins, R.C., Herzenberg, L.A., Negrin, R.S. & Contag, C.H. (2005). Molecular imaging using labeled donor tissues reveals patterns of engraftment, rejection, and survival in transplantation. Transplantation 80, 134-139.
127. Chikkanna-Gowda, C.P., Sheahan, B.J., Fleeton, M.N. & Atkins,G.J. (2005). Regression of mouse tumours and inhibition of metastases following administration of a Semliki Forest virus vector with enhanced expression of IL-12. Gene Therapy 12, 1253-1263.
126. Dodding, M.P., Bock, M., Yap, M.W. & Stoye, J.P. (2005). Capsid processing requirements for abrogation of Fv1 and Ref1 restriction. J. Virol. 79, 10571-10577.
125. Duxbury, R., Gilham,D.E., Russell, A., Woods, E., Ferguson, M.W.J., McKay, T.R. & Hawkins, R.E. (2005). A gene therapeutic approach to scar reduction at the site of cutaneous wounding. European Cells and Materials 10, 43.
124. Fang, J., Qian,J-J., Yi, S., Harding, T.C., Tu, G.H., VanRoey, M. & Jooss, K. (2005). Stable antibody expression at therapeutic levels using the 2A peptide. Nature Biotech. 23, 584-590.
123. Geiss, B.J., Pierson, T.C. & Diamond, M.S. (2005). Actively replicating West Nile virus is resistant to cytoplasmic delivery of siRNA. Virology J. 2, 53.
122. Heinkelein, M., Hoffmann, U., Lucke, M., Imrich, H., Muller, J.G., Meixensberger, J., Westphahl, M., Kretschmer, A. & Rethwilm, A. ( 2005). Experimental therapy of allogeneic solid tumors induced in athymic mice with suicide gene-transducing replication competent foamy virus vectors. Cancer Gene Ther. 12, 947-953.
121. Jones, M., Davidson, A., Hibbert, L., Gruenwald, P., Schlaak, J., Ball, S., Foster, G.R. & Jacobs, M. (2005). Dengue virus inhibits alpha interferon signaling by reducing STAT2 expression. J. Virol. 79, 5414-5420.
120. Klump, H., Schiedlmeier, B. & Baum, C. (2005). Control of self-renewal and differentiation of hematopoietic stem cells: HOXB4 on the threshold. Ann. N.Y. Acad. Sci. 1044, 6–15.
119. Lengler, J., Holzmuller, H., Sahnons, B., Gunzburg, W.H. & Renner, M. (2005). FMDV-2A sequence and protein arrangement contribute to functionality of CYP2B1-reporter fusion protein. Anal. Biochem. 343 116-124.
118. Osborn, M.J., Panoskaltsis-Mortari, A., McElmurry, R.T., Bell, S.K., Vignali, D.A.A., Ryan, M.D., Wilber, A., McIvor, R.S., Tolar, J. & Blazar, B.R. (2005). A picornaviral ‘2A-like’ sequence based tricistronic vector allowing for high level therapeutic gene expression coupled to a dual reporter system. Mol. Ther. 12, 569-574.
117. Pilat, S., Carotta, S., Schiedlmeier, B., Kamino, K., Mairhofer, A., Will, E., Modlich, U., Steinlein, P., Ostertag, W., Baum, C., Beug, H. & Klump, H. (2005). HOXB4 enforces equivalent fates of ES-cell-derived and adult hematopoietic cells. Proc. Natl. Acad. Sci. USA. 102, 12101-12106.
116. Rodriguez-Madoz, J.R., Prieto, J. & Smerdou, C. (2005). Semliki forest virus vectors engineered to express higher IL-12 levels induce efficient elimination of murine colon adenocarcinomas. Mol. Ther. 12, 153-163
115. Sinn, P.L., Sauter, S.L. & McCray, P.B. Jr. (2005). Gene therapy progress and prospects: development of improved lentiviral and retroviral vectors-design, biosafety, and production. Gene Ther. 12, 1089-1098.
114. Szymczak, A.L. & Vignali, D.A.A. (2005). Development of 2A peptide-based strategies in the design of multicistronic vectors. Expert Opinion 5, 627-638.
113. Szymczak, A.L. & Vignali, DA.A. (2005). Plasticity and rigidity in adaptor protein-2-mediated internalization of the TCR:CD3 complex. J. Immunol. 174, 4153-4160.
112. Szymczak, A.L., Workman, C.J., Gil, D., Dilioglou, S., Vignali, K.M., Palmer, E. & Vignali, D.A. (2005). The CD3 epsilon proline-rich sequence, and its interaction with Nck, is not required for T cell development and function. J. Immunol. 175, 270-275
111. Uhde, K., Fischer, R. & Commandeur, U. (2005). Expression of multiple foreign epitopes presented as synthetic antigens on the surface of Potato virus X particles. Arch. Virol. 150, 327–340
110. Yasuda, H., Tada, Y., Hayashi, Y., Jomori, T. & Takaiwa, F. (2005). Expression of the small peptide GLP-1 in transgenic plants. Transgenic Res. 14, 677-684.
2004
109. Ansari, I.H., Chen, L.M., Liang, D., Gil, L.H., Zhong, W. & Donis, R.O (2004). Involvement of a bovine viral diarrhea virus NS5B locus in virion assembly. J. Virol. 78, 9612-9623.
108. Arnold, P.Y., Burton, A.R. & Vignali, D.A.A. (2004). Diabetes incidence is unaltered in glutamate decarboxylase 65-specific TCR retrogenic nonobese diabetic mice: Generation by retroviral-mediated stem cell gene transfer. J. Immunol. 173, 3103-3111.
107. Blankenberg, F.G., Mandl, S., Cao, Y.A., O'Connell-Rodwell, C., Contag, C., Mari, C., Gaynutdinov, T.I., Vanderheyden, J.L., Backer, M.V. & Backer, J.M. (2004). Tumor imaging using a standardized radiolabeled adapter protein docked to vascular endothelial growth factor. J. Nucl. Med. 45, 1373-80.
106. de Felipe, P. (2004). Skipping the co-expression problem: the new 2A "CHYSEL" technology. Genet. Vaccines Ther. 2, 13.
105. de Felipe, P. & Ryan, M.D. (2004) Targeting of proteins derived from self-processing polyproteins containing multiple signal sequences. Traffic 5, 616-626.
104. El Amrani, A., Barakate, A., Askari, B.M., Li, X., Roberts, A.G., Ryan, M.D. & Halpin, C. (2004). Coordinate expression and independent subcellular targeting of multiple proteins from a single transgene. Plant Physiol. 135, 16-24.
103. Francois, I.E.J.A., Van Hemelrijck, W., Aerts, A.M., Wouters, P.F.J., Proost, .P, Broekaert, W.F. & Cammue, B.P.A. (2004). Processing in Arabidopsis thaliana of a heterologous polyprotein resulting in differential targeting of the individual plant defensins. Plant Sci. 166, 113-121.
102. Halpin, C. & Ryan, M.D. (2004). "Redirecting Metabolism by Co-ordinate Manipulation of Multiple Genes". In Metabolic Engineering in the Post-Genomic Era', Kholodenko, B. and Westerhoff, H. (eds.) Horizon Scientific Press, Norfolk, England.
101. Herd, K.A., Harvey, T., Khromykh, A.A. & Tindle, R.W. (2004). Recombinant Kunjin virus replicon vaccines induce protective T-cell immunity against human papillomavirus 16 E7-expresssing tumor. Virology 319, 237-248.
100. Hertzig, T., Scandella, E., Schelle, B., Ziebuhr, J., Siddell, S.G., Ludewig, B., & Thiel, V. (2004). Rapid identification of coronavirus replicase inhibitors using a selectable replicon RNA. J. Gen. Virol. 85, 1717-1725.
99. Honkavuori, K.S., Pollard, B.D., Rodriguez, M.S., Hay, R.T. & Kemp, G.D. (2004). Dual role of the adenovirus pVI C terminus as a nuclear localization signal and activator of the viral protease. J. Gen. Virol. 85, 3367-3376.
98. Karlsson, G.B. & Lijestrom, P. (2004), Delivery and expression of heterologous genes in mammalian cells using self-replicating alphavirus vectors. Methods in Mol. Biol. 246, 543-557.
97. Kwon, S.J., Hwang, E.W. & Kwon, H.B. (2004). Genetic engineering of drought resistant potato plants by co-introduction of genes encoding trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase of Zygosaccharomyces rouxii. Korean J. Genet. 26, 199-206.
96. Lorens, J.B., Pearsall, D.M., Swift, S.E., Peelle, B., Armstrong, R., Demo, S.D., Ferrick, D.A., Hitoshi, Y., Payan, D.G. & Anderson, D. (2004) Stable, stoichiometric delivery of diverse protein functions. J. Biochem. & Biophys Methods 58, 101-110.
95. Luke, G.A., de Felipe, P., Hughes, L.E. & Ryan, M.D. (2004). Skipping a turn. Biologist 51, 1-5.
94. Milsom, M.D., Woolford, L.B., Margison, G.P., Humphries, R.K. & Fairbairn, L.J. (2004). Enhanced in vivo selection of bone marrow cells by retroviral-mediated coexpression of mutant O6-methylguanine-DNA-methyltransferase and HOXB4. Mol. Ther. 10, 862-873.
93. Milsom, M.D. & Fairbairn, L.J. (2004). Protection and selection for gene therapy in the hematopoietic system. J. Gene Medicine 6, 133-146..
92. Nakaya, Y., Nakaya, T., Park, M.S., Cros, J., Imanishi, J., Palese, P. & Garcia-Sastre, A. (2004). Induction of cellular immune responses to simian immunodeficiency virus gag by two recombinant negative-strand RNA virus vectors. J. Virol. 78, 9366-9375.
91. Namy, O., Rousset, J.-P., Napthine, S. & Brierley, I. (2004). Reprogrammed genetic decoding in cellular gene expression. Mol. Cell 12, 157-168.
90. Radcliffe, P.A. & Mitrophanous, K.A. (2004). Multiple gene products from a single vector: 'self-cleaving' 2A peptides. Gene Therapy 11, 1673-1674.
89. Ralley, L., Enfissi, E.M.A., Misawa, N., Schuch, W., Bramley, P.M. & Fraser, P.D. (2004). Metabolic engineering of ketocarotenoid formation in higher plants. Plant J. 39 477-486.
88. Randall, J., Sutton, D., Ghoshroy, S., Bagga, S. &Kemp, J.D. (2004). Co-ordinate expression of beta- and delta-zeins in transgenic tobacco. Plant Sci. 167, 367-372.
87. Ryan, M.D., Donnelly, M.L.L., Flint, M., Cowton, V.M., Luke, G., Hughes, L.E., Knox, C. & de Felipe, P. (2004). "Foot-and-Mouth Disease Virus Proteinases". In Foot-And-Mouth Disease: current perspectives. E. Domingo & F. Sobrino (eds). Horizon Scientific Press, Norfolk, England. pp. 53-76.
86. Scholle, F., Girard, Y.A., Zhao, Q., Higgs, S. & Mason, P.W. (2004). trans-Packaged West Nile Virus-Like Particles: Infectious properties In Vitro and in infected mosquito vectors. J. Virol. 78, 11605-11614.
85. Sun, Q. & Han, H. (2004). Design and efficacy of a gene trap system for secretory proteins. Prog. Biochem. Biophys. 31, 328-333.
84. Szymczak, A.L., Workman, C.J., Wang, Y., Vignali, K.M., Dilioglou, S., Vanin, E.F. & Vignali, D.A. (2004). Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector. Nature Biotech. 22, 589-94.
83. Takeda, A., Kaido, M., Okuno, T. & Mise, K. (2004). The C terminus of the movement protein of Brome mosaic virus controls the requirement for coat protein in cell-to-cell movement and plays a role in long-distance movement. J. Gen. Virol. 85, 1751-1761.
82. Tessitore, A., del P Martin, M., Sano, R., Ma, Y., Mann, L., Ingrassia, A., Laywell, E.D., Steindler, D.A., Hendershot, L.M. & d'Azzo, A. (2004). GM1-ganglioside-mediated activation of the unfolded protein response causes neuronal death in a neurodegenerative gangliosidosis. Mol Cell. 15, 753-66.
2003
81. Baranov, P.V., Gurvich, O.L., Hammer, A.W., Gesteland, R.F. & Atkins, J.F. (2003). RECODE 2003. Nucleic Acids Res. 31, 87-89.
80. Binder, J.J., Hoffman, M.A. & Palmenberg, A.C. (2003). Genetic stability of attenuated mengovirus vectors with duplicate primary cleavage sequences. Virology 312, 481-494.
79. de Felipe, P. & Izquierdo, M. (2003). Construction and characterization of pentacistronic retrovirus vectors. J. Gen. Virol. 84, 1281–1285.
78. de Felipe, P. Hughes, L.E. Ryan, M.D. & Brown, J.D. (2003). Co-translational, intraribosomal cleavage of polypeptides by the foot-and-mouth disease virus 2A peptide. J. Biol. Chem. 278, 11441-11448.
77. Harvey, T.J., Anraku, I., Linedale, R., Harrich, D., Mackenzie, J., Suhrbier, A. & Khromykh, A.A. (2003). Kunjin virus replicon vectors for human immunodeficiency virus vaccine development. J Virol. 77, 7796-7803.
76. Jackson, C.A, Messinger, J., Palmer, M.T., Peduzzi, J.D. & Morrow, C.D. (2003). Gene expression in the muscle and central nervous system following intramuscular inoculation of encapsidated or naked poliovirus replicons. Virology 314, 45-61.
75. Martin, E., Kamath, A.T., Briscoe, H., & Britton, W.J. (2003) The combination of plasmid interleukin-12 with a single DNA vaccine is more effective than Mycobacterium bovis (bacille Calmette-Guerin) in protecting against systemic Mycobacterim avium infection. Immunology 109, 308-314.
74. Nakaya, Y., Zheng, H. & Garcia-Sastre, A. (2003) Enhanced cellular immune responses to SIV Gag by immunization with influenza and vaccinia virus recombinants. Vaccine 21, 2097-2106.
73. Novak, M.J., Moldoveanu, Z., Huang, W.Q., Jackson, C.A., Palmer, M.T., McPherson, S.A. & Morrow, C.D. (2003) Intramuscular immunization with poliovirus replicons primes for a humoral and cellular immune response to soluble antigen. Viral Immunology 16, 169-182.
72. Schiedlmeier, B., Klump, H., Will, E., Arman-Kalcek, G., Li, Z., Wang, Z., Rimek, A., Friel, J., Baum, C. & Ostertag, W. (2003). High-level ectopic HOXB4 expression confers a profound in vivo competitive growth advantage on human cord blood CD34+ cells, but impairs lymphomyeloid differentiation. Blood 101, 1759-1768.
71. Shi, P-Y. (2003)/ Genetic systems of West Nile virus and their potential applications. Curr. Op. in Investigational Drugs 4, 959-965.
70. Straathof, K.C., Spencer, D.M., Sutton, R.E. & Rooney, C.M. (2003). Suicide genes as safety switches in T lymphocytes. Cytotherapy 5, 227-230.
69. Thomas, J.M., Klimstra, W.B., Ryman, K.D. & Heidner, H.W. (2003) Sindbis virus vectors designed to express a foreign protein as a cleavable component of the viral structural polyprotein. J. Virol. 77, 5598-5606.
68. Ward, S.M., Tindle, R.W., Khromykh, A.A. & Gowans, E.J. Generatino of CTL responses using Kunjin replicon RNA. Immunol. Cell Biol. 81, 73-78.
2002
67. Anraku, I., Harvey, T.J., Linedale, R., Gardner, J., Harrich, D., Suhrbier, A. & Khromykh, A.A. (2002). Kunjin virus replicon vaccine vectors induce protective CD8+ T-cell immunity. J. Virol. 76, 3791-3799.
66. Awram, P., Gardner, R.C., Forster, R.L. & Bellamy, A.R. (2002). The potential of plant viral vectors and transgenic plants for subunit vaccine production. Adv. Virus Res. 58, 81-124.
65. Contag, C.H. & Bachmann, M.H. (2002). Advances in in vivo bioluminescence imaging of gene expression. Annu. Rev. Biomed. Eng. 4, 235-260.
64. de Felipe, P. (2002). Polycistronic viral vectors. Curr Gene Ther. 2, 355-378.
63. François, I.E.J.A., Broekaert, W.F. & Cammue, B.P.A. (2002a). Different approaches for multi-stacking in plants. Plant Sci. 163, 281-295.
62. François, I.E., De Bolle, M.F., Dwyer, G., Goderis, I.J., Woutors, P.F., Verhaert, P.D., Proost, P., Schaaper, W.M., Cammue, B.P. & Broekaert, W.F. (2002). Transgenic expression in Arabidopsis of a polyprotein construct leading to production of two different antimicrobial proteins. Plant Physiol. 128, 1346-1358.
61. Hope, J.C., Kwong, L.S., Entrican, G., Wattegedera, S., Vordermeier, H.M., Sopp, P. & Howard, C.J. (2002) Development of detection methods for ruminant interleukin (IL)-12. J. Immunological Methods 266, 117-126.
60. Kinsella, T.M., Ohashi, C.T., Harder, A.G., Yam, G.C., Li, W., Peelle, B., Pali, E.S., Bennett, M.K., Molineaux, S.M., Anderson, A., Masuda, E.S. & Payan, D.G. (2002) Retrovirally delivered random cyclic peptide libraries yield inhibitors of interleukin-4 signalling in human B cells. J. Biol. Chem. 277, 37512-37518.
59. Liu, W.J., Sedlak, P.L., Kondratieva, N. & Khromykh, A.A. (2002). Complementation analysis of the flavivirus Kunjin NS3 and NS5 proteins defines the minimal regions essential for formation of a replication complex and shows a requirement of NS3 in cis for virus assembly. J. Virol. 76, 10766-10755.
58. Ma, C. & Mitra, A. (2002) Expressing multiple genes in a single open reading frame with the 2A region of foot-and-mouth disease virus as a linker. Molecular Breeding 9, 191-199.
57. Ma, C & Mitra, A. (2002) Instrinsic direct repeats generate consistent post-transcriptional gene silencing in tobacco. The Plant Journal 31, 37-49.
56. Mlotshwa, S., Verver, J., Sithole-Niang, I., Gopinath, K., Carette, J., van Kammen, A. & Wellink J. (2002). Subcellular location of the helper component-proteinase of cowpea aphid-borne mosaic virus. Virus Genes 25, 207-216.
55. Palendira, U., Kamath, A.T., Feng, C.G., Martin, E., Chaplin, P.J., Triccas, J.A. & Britton, W.J. (2002). Coexpression of interleukin-12 chains by a self-splicing vector increases the protective cellular immune response of DNA and Mycobacterium bovis BCG vaccines against Mycobacterium tuberculosis. Infection and Immunity 70, 1949-1956.
54. Pouwels, J., Carette, J.E., van Lent, J. & Wellink, J. (2002). Cowpea mosiac virus: effects on host cell processes. Mol. Plant. Pathol. 3, 411-418.
53. Rayner, J.O., Dryga, S.A. & Kamrud, K.I. (2002). Alphavirus vectors and vaccination. Rev. Med. Virol. 12, 279-296.
52. Ryan, M.D., Luke, G., Hughes, L.E., Cowton, V.M., ten Dam, E., Li, X., Donnelly, M.L.L., Mehrotra, A. & Gani, D. (2002). The aphtho- and cardiovirus ‘primary’ 2A/2B polyprotein ‘cleavage’. In ‘Molecular Biology of Picornaviruses’ Semler, B. and Wimmer, E. (eds.) pp. 213-223. ASM Press, Washington, USA.
51. Takasuka, N., Enami, M., Itamura, S. & Takemori, T. (2002). Intranasal inoculation of a recombinant influenza virus containing exogenous nucleotides in the NS segment induces mucosal immune response against the exogenous gene product in mice. Vaccine 20, 1579-1585.
50. Triccas, J.A., Sun, L., Palendira, U. & Britton, W.J. (2002) Comparative affects of plasmid-encoded interleukin 12 and interleukin 18 on the protective efficacy of DNA vaccination against Mycobacterium tuberculosis. Immunol. Cell Biol. 80 346-350.
49. Yi, M.K., Bodola, F. & Lemon, S.M. (2002) Subgenomic hepatitis C virus replicons inducing expression of a secreted enzymatic reporter protein. Virology 304, 197-210.
2001
48. Ansardi, D.C., Porter, D.C., Jackson, C.A., Gillespie, G.Y. & Morrow, C.D. (2001). RNA replicons derived from poliovirus are directly oncolytic for human tumor cells of diverse origins. Cancer Res. 61, 8470-8479.
47. Essenberg, M. (2001). Prospects for strengthening plant defenses through phytoalexin engineering. Physiol. Mol. Plant Pathol. 59, 71-81.
46. Furler, S., Paterna, J.C., Weibel, M., & Bueler H. (2001). Recombinant AAV vectors containing the foot and mouth disease virus 2A sequence confer efficient bicistronic gene expression in cultured cells and rat substantia nigra neurons. Gene Therapy 8, 864-873.
45. Halpin, C., Barakate, A., Askari, B., Abbott, J. & Ryan, M.D. (2001). Enabling technologies for manipulating multiple genes on complex pathways. Plant Mol. Biol. 47, 295-310.
44. Hunt, A.G. & Maiti, I.B. (2001). Strategies for expressing multiple foreign genes in plants as polycistronic constructs. In Vitro Cell. Dev. Biol. 37, 313-320.
43. Jackson, C. A., Cobbs, C., Peduzzi, J.D., Novak, M. & Morrow, C. D. (2001). Repetitive intrathecal injections of poliovirus replicons result in gene expression in neurons of the Central Nervous System without pathogenesis. Hum. Gene Ther. 12, 1,827-1,841.
42. Klump, H., Schiedlmeier, B., Vogt, B., Ryan, M.D, Ostertag, W. & Baum, C. (2001). Retroviral vector-mediated expression of HoxB4 in hematopoietic cells using a novel coexpression strategy. Gene Therapy 8, 811-817.
41. Luke, G. & Ryan M.D. (2001). Translating the message. Biologist 48, 79-82.
40. Pang, X., Zhang, M. & Dayton, A.I (2001). Development of dengue virus replicons expressing HIV-1 gp120 and other heterologous genes: a potential future tool for dual vaccination against dengue virus and HIV. BMC Microbiol. 1 1-28.
39. Sanchez-Navarro, J., Miglino, R., Ragozzino, A. & Bol, J.F. (2001). Engineering of Alfalfa mosaic virus RNA 3 into an expression vector. Arch. Virol. 146, 923-939.
2000
38. Bramel-Verheije, M.H.G., Rottier, P.J.M., & Meulenberg, J.J.M. (2000). Expression of a foreign epitope by porcine reproductive and respiratory syndrome virus. Virology 278, 380-389.
37. de Felipe, P. & Izquierdo, M. (2000). Tricistronic and tetracistronic retroviral vectors for gene transfer. Human Gene Therapy 11, 1921-1931.
36. de Rose, R., Scheerlinck, J.P.Y., Casey, G,, Wood, P.R., Tennent, J,M,, & Chaplin, P.J. (2000). Ovine interleukin-12: Analysis of biologic function and species comparison. J. Interferon & Cytokine Res. 20, 557-564.
35. García-Sastre, A. (2000). Transfectant influenza viruses as antigen delivery vectors. Adv. Virus Res. 55, 579-597.
34. Gopinath, K., Wellink, J., Porta, C., Taylor, K.M., Lomonossoff, G.P., & van Kammen, A. (2000). Engineering cowpea mosaic virus RNA-2 into a vector to express heterologous proteins in plants. Virology 267, 159-173.
33. Hewson, R. RNA viruses: emerging vectors for vaccination and gene therapy. Mol. Med. Today 6, 28-35.
32. Khromykh, A.A. (2000). Replicon-based vectors of positive strand RNA viruses. Curr. Opin. Mol. Therapeutics 2, 555-569.
31. O'Brien, G.J., Bryant, C.J., Voogd, C., Greenberg, H.B., Gardner, R.C, & Bellamy, A.R. (2000). Rotavirus VP6 expressed by PVX vectors in Nicotiana benthamiana coats PVX rods and also assembles into virus like particles. Virology 270, 444-453.
30. Suzuki, N., Geletka, L.M., & Nuss, D.L. (2000). Essential and dispensable virus-encoded replication elements revealed by efforts to develop hypoviruses as gene expression vectors. J. Virol. 74, 7568-7577.
29. Thomas, C.L. & Maule, A.J. (2000). Limitations on the use of fused green fluorescent protein to investigate structure-function relationships for the cauliflower mosaic virus movement protein. J. Gen. Virol. 81, 1851-1855.
28. Varnavski, A.N., Young, P.R. & Khromykh, A.A. (2000). Stable high-level expression of heterologous genes in vitro and in vivo by noncytopathic DNA-based Kunjin virus replicon vectors. J. Virol. 74, 4394-4403.
27. Wang, T.H., Rijnbrand, R.C.A. & Lemon, S.M. (2000). Core protein-coding sequence, but not core protein, modulates the efficiency of cap-independent translation directed by the internal ribosome entry site of hepatitis C virus. J. Virol. 74, 11,347-11,358.
26. Chaplin, P.J., Camon, E.B., Villarreal-Ramos, B., Flint, M., Ryan, M.D. & Collins, R.A. (1999). Production of interlukin-12 as a self-processing polypeptide. J. Interferon & Cytokine Res. 19, 235-241.
25. de Felipe, P., Martín, V., Cortés, M.L., Ryan, M.D. & Izquierdo, M. (1999). Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy. Gene Therapy 6, 198-208.
24. Halpin, C., Cooke, S.E., Barakate, A., El Amrani, A. & Ryan, M.D. (1999). Self-processing polyproteins - a system for co-ordinate expression of multiple proteins in transgenic plants. Plant J. 17, 453-459.
23. Kokuho T, Watanabe S, Yokomizo Y, Inumaru S (1999). Production of biologically active, heterodimeric porcine interleukin-12 using a monocistronic baculoviral expression system. Vet. Immunol. Immunopathol. 72, 289-302.
22. Smerdou, C. & Liljestrom, P. (1999). Two-helper RNA system for production of recombinant Semliki Forest Virus particles. J. Virol. 73, 1092-1098.
21. Turpen, T.H. (1999). Tobaco mosaic virus and the virescence of biotechnology. Phil. Trans. R. Soc. Lond. B 354, 665-673.
20. Varnavski, A.N. & Khromykh. A,A. (1999). Noncytopathic flavivirus replicon RNA-based system for expression and delivery of heterologous genes. Virology 255, 366-375.
19. Bock, M., Heinkelein, M., Lindemann, D. & Rethwilm, A. (1998). Cells expressing the human foamy virus (HFV) accessory Bet protein are resistant to productive HFV superinfection. Virology 250, 194-204.
18.Collins, R.A., Camon, E.B., Chaplin, P.J. & Howard, C.J. (1998). Influence of IL-12 on interferon-gamma production by bovine leucocyte subsets in response to bovine respiratory syncytial virus. Vet. Imunol. Immunopath. 63, 69-72.
17. Halpin, C. & Ryan, M.D. (1998). Expression of self processing polyproteins in transgenic plants. U.S. Patent 5,846,767. Assignee - Zeneca Limited, London (PCT No. PCT/ GB94/ 02765).
16. Porta, C. & Lomonossoff, G.P. (1998). Scope for using plant viruses to present epitopes from animal pathogens. Rev. Med. Virol. 8, 25-41.
15. Scheerlinck, J.-P. Y., Chaplin, P.J. & Wood, P.R. (1998). Ovine cytokines and their role in the immune response. Vet. Res. 29, 369-383.
14. Smolenska, L., Roberts, L.M., Learmonth, D., Porter, A.J., Harris, W.J., Wilson, T.M.A., & Cruz, S. S. (1998). Production of a functional single chain antibody attached to the surface of a plant virus. Febs Letters 441, 379-382.
13. van der Ryst, E., Nakasone, T., Habel, A., Venet, A., Gomard, E., Altmeyer,R., Girard, M, & Borman, A.M. (1998). Study of the immunogenicity of different recombinant Mengo viruses expressing HIV1 and SIV epitopes. Res. in Virol. 149, 5-20.
12. Camon, E.B., Chaplin. P.J., Howard, C.J. & Collins, R.A. (1997). Modulation of bovine T cell responses by IL-12 and the influence of IL-4 on interferon-gamma responses to respiratory syncytial virus. Biochem. Soc. Trans. 25, S291.
11. Jonson, J., Lin, T. & Lomonossoff, G. (1997). Presentation of heterologous peptides on plant viruses: genetics, structure, and function. Ann. Rev. Phytopathol. 35, 67-86.
10. Nestler, U., Heinkelein, M., Lucke, M., Meixensberger, J., Scheurlen, W., Kretschmer, A. & Rethwilm, A. (1987). Foamy virus vectors for suicide gene therapy. Gene Therapy 4, 1270-1277.
9. Roberts, A.G., Santa Cruz, S., Roberts, I M., Prior, D.A.M., Turgeon, R. and Oparka, K.J. (1997). Phloem unloading in sink leaves of Nicotiana benthamiana: Comparison of a fluorescent solute with a fluorescent virus. The Plant Cell 9, 1381-1396.
8. Cruz, S.S., Chapman, S., Roberts, A.G., Roberts, I.M., Prior, D.A.M., & Oparka,K.J. (1996). Assembly and movement of a plant-virus carrying a green fluorescent protein overcoat. Proc. Natl. Acad. Sci. U.S.A. 93, 6286-6290.
7. Mattion, N.M., Harnish, E.C., Crowley, J.C. & Reilly, P.A. (1996). Foot-and-Mouth Disease Virus 2A protease mediates cleavage in attenuated Sabin 3 poliovirus vectors engineered for delivery of foreign antigens. J. Virol. 70, 8124-8127.
6. García-Sastre, A. & Palese, P. (1995). Influenza virus vectors. Biologicals 23, 171-178.
5. Oparka, K.J., Roberts, A.G., Prior, D.A.M., Chapman, S., Baulcombe, D. & Santa Cruz, S. (1995). Imaging the green fluorescent protein in plants - viruses carry the torch. Protoplasma 189, 133-141.
4. Precious, B., Young, D.F., Bermingham, A., Fearns, R., Ryan, M.D. & Randall, R.E. (1995). Inducible expression of the P, V, and NP genes of the paramyxovirus simian virus 5 in cell lines and an examination of the NP-P and NP-V interactions. J. Virol. 69, 8001-8010.
3. Schmidt, M. & Rethwilm, A. (1995). Replicating Foamy Virus-based vectors directing high-level expression of foreign genes. Virology 210, 167-178.
2. Percy, N., Barclay, W.S., Garcia-Sastre, A. & Palese, P. (1994). Expression of a foreign protein by influenza A virus. J. Virol. 68, 44486-4492.
1. Ryan, M.D. & Drew, J. (1994). Foot-and-mouth disease virus 2A oligopeptide mediated cleavage of an artificial polyprotein. EMBO J. 134, 928-933.