2A-Mediated Cleavage

Mechanism of 'Cleavage'

BBSRC-funded research into the involvement of translation release (eRF1 and eRF3) factors in the cleavage mechanism

Wellcome Trust-funded research into the mechanism of the cleavage: the role of elongation factor 2 (eEF2) in the synthesis of proteins downstream of 2A

Images of co-expressed cyan and yellow fluorescent proteins targeted to different sub-cellular sites .


(introduction to the system)

Published Biotechnological Applications of the 2A System

2A and 2A-like Sequences (Adobe Acrobat file)


Group Members

Group Publications

e-mail : Martin Ryan

Other Contact Details

 

 

 

 

 

 

 

 

 

 

2A co-expression technology
*NEW* 2A used by many laboratories in the production of induced pluripotent stem (iPS) cells
- the use of 2A in gene therapies
- the use of 2Ain bioimaging
- the use of 2A in the creation of genetically-modified organisms

Teaching Material:

BL3302 - Gene Regulation

BL4210 - Practical Skills

BL4213 - Molecular Virology