Biologics

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We have subcloned the CBM40 domain of the V. cholerae sialidase that recognises sialic acid with micromolar affinity. By linking 2, 3 or 4 CBM40 modules we can enhance the affinity 15,000-fold to 4 nanomolar, measured by SPR. The domain recognises just the terminal sialic acid, irrespective of linkage, confirmed by the crystal structure of the isloated CBM40 domain that shows the compete electron density for sialyllactose. (Connaris, Crocker & Taylor (2009) J. Biol. Chem. 284, 7339-7351)

 

Increasing carbohydrate affinity through multivalency

CBM40

PDB code: 2w68http://www.rcsb.org/pdb/explore.do?structureId=2W68