A Day in the Life of ...
7:00 Get up and get ready for work
8.30 Arrive at office, go through e-mails, make coffee for the
office, meet with colleagues to coordinate schedules so nobody will be
stuck waiting for any of the automated platforms.
9.30 Defrost samples (e.g. cDNA previously synthesized
from RNA extracted from whole blood of prostate cancer patients) and set
up lab bench for work. Organize paperwork for the samples to be
processed, create a schedule of tasks and timing for any colleagues
assisting with the project
10.30 With samples thawed and ready for use, begin lab
work (e.g. dilute cDNA and distribute into appropriate aliquots
according to previously prepared calculations and layouts for loading
onto Real-Time Quantitative PCR gene panels)
12.00 Break for lunch
12.30/1.00 Head back into the lab to finish lab work (e.g.
use the automated liquid handlers to load the diluted cDNA into 96-well
gene template plates, and convert to 384-well qPCR plates
3.30 Prepare calculations and layouts for the next day;
review gene expression data generated from previously-run qPCR plates
and enter into LIMS (laboratory information management system)
5.00 Go Home. Or, if there are administrative tasks
needing attention, I stay late to work on those. We have an extensive
LIMS system and a lot of required paperwork because every sample must be
traceable and auditable from receipt of the whole blood or tissue
sample, all the way through to the generation of qPCR gene expression
data. Often times in the middle of a large project such as the one I’m
currently working on, lab work takes up so much of the day that the
paperwork and admin tends to get neglected, so I try to stay late or
come in early or on the weekend to catch up.
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