Structural biology of enzymes and membrane proteins

Group leader: Jim Naismith

Professor of Chemical Biology and Director of Biomedical Sciences Research Complex

Research overview

The application of protein structure determination (by X-ray crystallography) coupled to molecular biology and biochemistry to probe biological mechanisms and to target specific disease pathways. We have on going research in 1) signal transduction 2) physical basis of protein carbohydrate interactions 3) pathogenic bacteria glycan assembly, we have cloned and crystallised a number of the enzymes involved in key steps of glycan synthesis in pathogenic bacteria. 4) viral replication, we are purifying and crystallising a hetero dimer crucial to replication of viruses in vivo (5) the biosynthesis of unusual natural products

Publications

Juárez, JF, Liu, H, Zamarro, MT, McMahon, S, Liu, H , Naismith, J , Eberlein, C, Boll, M, Carmona, M & Diaz, E 2015, ' Unraveling the specific regulation of the mdb central pathway for the anaerobic degradation of 3-methylbenzoate ' Journal of Biological Chemistry , vol 290, pp. 12165-12183.
Hageluken, G, Clarke, BR , Huang, H , Tuukkanen, A, Danciu, I, Svergun, DI, Hussain, R, Liu, H, Whitfield, C & Naismith, JH 2015, ' A coiled-coil domain acts as a molecular ruler to regulate O-antigen chain length in lipopolysaccharide ' Nature Structural and Molecular Biology , vol 22, no. 1, pp. 50-56.
Koehnke, JAJG , Mann, G, Bent, AF, Ludewig, H , Shirran, SL , Botting, CH , Lebl, T , Houssen, W, Jaspers, M & Naismith, J 2015, ' Structural analysis of leader peptide binding enables leader-free cyanobactin processing ' Nature Chemical Biology , vol Advance Online.
Branigan, E, Plechanovová, A, Jaffray, E , Naismith, J & Hay, RT 2015, ' Structural basis for the RING-catalyzed synthesis of K63-linked ubiquitin chains ' Nature Structural and Molecular Biology , vol In press.
Houssen, WE, Bent, AF, McEwan, AR, Pieiller, N, Tabudravu, J , Koehnke, J , Mann, G, Adaba, RI, Thomas, L, Hawas, UW, Liu, H , Schwarz-Linek, U , Smith, MCM , Naismith, JH & Jaspars, M 2014, ' An efficient method for the in vitro production of Azol(in)e-based cyclic peptides ' Angewandte Chemie International Edition , vol 53, no. 51, pp. 14171-14174.

Overview

Overview header image

Scientists associated with the thirty-two research groups that are affiliated with the Biomedical Sciences Research Complex perform highly innovative, multi-disciplinary research in eleven broad areas of biomedical research, employing state-of-the-art techniques to address key questions at the leading edge of the biomedical and biological sciences. The BSRC is grateful for funding from all funding agencies including the Institutional Strategic Support Fund from the Wellcome Trust.

Follow the links on the left to view individual research groups associated with one or more of the eleven BSRC research areas.

Research areas

Scientists associated with the thirty-two research groups that are affiliated with the Biomedical Sciences Research Complex perform highly innovative, multi-disciplinary research in eleven broad areas of biomedical research, employing state-of-the-art techniques to address key questions at the leading edge of the biomedical and biological sciences.

Follow the links on the left to view individual research groups associated with one or more of the eleven BSRC research areas.

Research by academic schools

Research in the BSRC is conducted by thirty-two independent research groups based in the Schools of Biology, Chemistry, Physics and Astronomy, and Medicine. Follow the links on the left to view groups associated with each school.