Structural biology of enzymes and membrane proteins

Group leader: Jim Naismith

Professor of Chemical Biology and Director of Biomedical Sciences Research Complex

Research overview

The application of protein structure determination (by X-ray crystallography) coupled to molecular biology and biochemistry to probe biological mechanisms and to target specific disease pathways. We have on going research in 1) signal transduction 2) physical basis of protein carbohydrate interactions 3) pathogenic bacteria glycan assembly, we have cloned and crystallised a number of the enzymes involved in key steps of glycan synthesis in pathogenic bacteria. 4) viral replication, we are purifying and crystallising a hetero dimer crucial to replication of viruses in vivo (5) the biosynthesis of unusual natural products

Publications

Hageluken, G, Clarke, BR , Huang, H , Tuukkanen, A, Danciu, I, Svergun, DI, Hussain, R, Liu, H, Whitfield, C & Naismith, JH 2015, ' A coiled-coil domain acts as a molecular ruler to regulate O-antigen chain length in lipopolysaccharide ' Nature Structural and Molecular Biology , vol 22, no. 1, pp. 50-56.
Houssen, WE, Bent, AF, McEwan, AR, Pieiller, N, Tabudravu, J , Koehnke, J , Mann, G, Adaba, RI, Thomas, L, Hawas, UW, Liu, H , Schwarz-Linek, U , Smith, MCM , Naismith, JH & Jaspars, M 2014, ' An efficient method for the in vitro production of Azol(in)e-based cyclic peptides ' Angewandte Chemie International Edition , vol 53, no. 51, pp. 14171-14174.
Naismith, JH & Parker, EJ 2014, ' Editorial overview: catalysis and regulation: enzyme catalysis, biosynthetic pathways and regulation ' Current Opinion in Structural Biology , vol 29, pp. 4-5.
Koehnke, JAJG , Bent, AF, Houssen, WE, Mann, G, Jaspars, M & Naismith, J 2014, ' The structural biology of patellamide biosynthesis ' Current Opinion in Structural Biology , vol 29, pp. 112-121.
Mann, G , Koehnke, JAJG , Bent, AF, Graham, R, Houssen, W, Jaspars, M , Schwarz-Linek, U & Naismith, J 2014, ' The structure of the cyanobactin domain of unknown function from PatG in the patellamide gene cluster ' Acta Crystallographica. Section F, Structural biology and crystallization communications , vol F70, pp. 1597-1603.

Overview

Scientists associated with the thirty-two research groups that are affiliated with the Biomedical Sciences Research Complex perform highly innovative, multi-disciplinary research in eleven broad areas of biomedical research, employing state-of-the-art techniques to address key questions at the leading edge of the biomedical and biological sciences. The BSRC is grateful for funding from all funding agencies including the Institutional Strategic Support Fund from the Wellcome Trust

Follow the links on the left to view individual research groups associated with one or more of the eleven BSRC research areas.

Research areas

Scientists associated with the thirty-two research groups that are affiliated with the Biomedical Sciences Research Complex perform highly innovative, multi-disciplinary research in eleven broad areas of biomedical research, employing state-of-the-art techniques to address key questions at the leading edge of the biomedical and biological sciences.

Follow the links on the left to view individual research groups associated with one or more of the eleven BSRC research areas.

Research by academic schools

Research in the BSRC is conducted by thirty-two independent research groups based in the Schools of Biology, Chemistry, Physics and Astronomy, and Medicine. Follow the links on the left to view groups associated with each school.