Enzyme promiscuity

The PDB code links will take you to the PDB entry with links to the publication

 
 

In a collaboration with Mike Danson, David Hough and Steven Bull at the University of Bath, we have been studying enzymes that are promiscuous for carbohydrate substrates from the hyperthermophilic archaeon Sulfolobus solfataricus.

 

Glucose dehydrogenase

PDB codes: 2cd9 (apo),http://www.rcsb.org/pdb/explore/explore.do?structureId=2CD9
2cda (NADP+),http://www.rcsb.org/pdb/explore/explore.do?structureId=2CDA
2cdb (glucose),http://www.rcsb.org/pdb/explore/explore.do?structureId=2CDB
2cdc (xylose),http://www.rcsb.org/pdb/explore/explore.do?structureId=2CDC

KDG aldolase

PDB codes: 1w3i (pyruvate), http://www.rcsb.org/pdb/explore/explore.do?structureId=1W3I
1w3n (D-KDG),http://www.rcsb.org/pdb/explore/explore.do?structureId=1W3N
1w3t (D-KDGal, D-glyceraldehyde, pyruvate)http://www.rcsb.org/pdb/explore/explore.do?structureId=1W3T
1w37 (apo)http://www.rcsb.org/pdb/explore/explore.do?structureId=1W37

KDG kinase

PDB codes: 2v78 (apo),http://www.rcsb.org/pdb/explore/explore.do?structureId=2V78
2var (AMPNP + KDG)http://www.rcsb.org/pdb/explore/explore.do?structureId=2VAR