School of Biology/Centre for Biomolecular Sciences

University of St. Andrews

* = corresponding author

 

Ribozymes

(1) Schiemann, O.*; Fritscher, J.; Kisseleva, N.; Sigurdsson, S. T.; Prisner, T. F. „Structural Investigation of a High Affinity Mn^II Binding Site in the Hammerhead Ribozyme by EPR Spectroscopy and DFT Calculations. Effects of Neomycine-B on Metal-Ion Binding“ ChemBioChem 2003, 4, 1057.

 

(2) Kisseleva, N.; Khvorova, A.; Westhof, E.; Schiemann, O.* „Binding of Manganese(II) to an Extended Hammerhead Ribozyme as studied by Electron Paramagnetic Resonance Spectroscopy” RNA 2005, 11, 1.

 

(3) Schiemann, O.*; Carmieli, R.; Goldfarb, D. „W-Band ³¹P-ENDOR on the High-Affinity Mn²⁺-Binding Site in the Minimal and Tertiary Stabilized Hammerhead Ribozymes“ Appl. Magn. Reson. 2007, 31, 543-552.

 

(4) Kisseleva, N.; Kraut, S.; Jäschke, A.*; Schiemann, O.* “Characterizing multiple metal ion binding sites within a ribozyme by Cadmium-induced EPR silencing” HFSPJ 2007, 1, 127-136.

 

(5) Wolfson, A.*; Kisseleva, N.; Khvorova, A.; Westhof, E.; Schiemann, O.* „Correlation of the fast cleavage behaviour of the minimal and tertiary stabilized hammerhead ribozymes derived from Tobacco Ringspot Virus RNA with EPR data“ Oligonucleotides 2008, 18, 101-110.

 

 

PELDOR

(6) Weber, A.; Schiemann, O.*; Bode, B.; Prisner, T. F. „PELDOR at S- and X-Band Frequencies and the Separation of Exchange Coupling from Dipolar Coupling“ J. Magn. Res. 2002, 157, 277.

 

(7) Schiemann, O.*; Weber, A.; Edwards, T. E.; Prisner, T. F.; Sigurdsson, S. T. „Nanometer Distance Measurements on RNA using PELDOR“ J. Am. Chem. Soc. 2003, 125, 3434.

 

(8) Schiemann, O.*; Piton, N.; Mu Y.; Stock, G.; Engels. J. W.; Prisner, T. F. “A PELDOR Based Nanometer Distance Ruler for Oligonucleotides“ J. Am. Chem. Soc. 2004, 126, 5722.

 

(9) Bode, B.; Margraf, D.; Plackmeyer, J. Dürner, G.; Prisner, T. F.; Schiemann, O.* „Counting the Monomers in Nanometer Sized Oligomers“ J. Am. Chem. Soc. 2007, 129, 6736-6745.

 

(10) Piton, N.; Mu, Y.; Stock, G.; Prisner, T. F.; Schiemann, O.*; Engels, J. W.* „Base-specific spin labeling of RNA for structure determination“ Nucl. Acids Res. 2007, 35, 3128-3143. CORRIGENDUM Nucl. Acids Res. 2007, 35, 5985.

 

(11) Schiemann, O.*; Prisner, T. F.* „Applications of electron paramagnetic resonance to distance measurements in biomolecules“ Quaterly Reviews of Biophysics 2007, 40, 1-53.

 

(12) D. Margraf, B.E. Bode, A. Marko, O. Schiemann, T.F. Prisner* „Conformational flexibility of nitroxide biradicals determined by X-band PELDOR experiments” Mol. Phys. 2007, 105, 2153-2160.

 

(13) Bode, B. E.; Plackmeyer, J.; Prisner, T. F.; Schiemann, O.* „PELDOR measurements on a Nitroxide Labeled Cu(II) Porphyrin: Orientation Selection, Spin-Density Distribution and Conformational Flexibility“ J. Phys. Chem. A 2008, 112, 5064-5073.

 

(14) Bode, B. E.; Plackmeyer, J.; Prisner, T. F.; Schiemann, O.* „PELDOR on an Exchange Coupled Nitroxide Copper(II) Spin Pair“ J. Organometallics 2009, 694, 1172–1179.

 

(15) Hagelueken, G.; Ingledew, W.J; Huang, H.; Petrovic-Stojanovska, B.; Whitfield, C.; ElMkami, H.; Schiemann, O.;^* Naismith, J.H.^* „PELDOR distance fingerprinting of the octameric outer-membrane protein Wza from Escherichia coli.“ Angew. Chem. Int. Ed. 2009, 121, 2948-2950.

 

(16) Schiemann, O.*; Cekan, P.; Margraf, D.; Prisner, T.F.; Sigurdsson, S.T.* “Relative orientation of rigid nitroxides by PELDOR:  Beyond distance measurements in nucleic acids” Angew. Chem. Int. Ed. 2009, 121, 3342-3345.

 

(17)  Margraf, D.; Cekan, P.; Prisner, T.F.; Sigurdsson, S.T.*; Schiemann, O.* "Ferro- and Antiferromagnetic Exchange Coupling Constants in PELDOR Spectra" Phys. Chem. Chem. Phys. 2009, accepted.

 

 

Synthesis/Spin-Labeling

(18) Strube, T.; Schiemann, O.; MacMillan, F.; Prisner, T. F., Engels, J. W.* „A new facile method for spin-labeling of oligonucleotides” Nucleos. Nucleot. Nucleic. 2001, 20, 1271.

 

(19) Fritscher, J.; Beyer, M.; Schiemann, O.* „Synthesis, Crystal Structure and Magnetic Properties of a Novel Nitroxide Biradical. Theoretical Investigation of the Exchange Mechanisms” Chem. Phys. Lett. 2002, 364, 393.

 

(20) Beyer, M.; Fritscher, J.; Feresin, E.; Schiemann, O.* „Synthesis of Novel Aromatic Nitroxides as Potential DNA Intercalators. An EPR Spectroscopical and DFT Computational Study“ J. Org. Chem. 2003, 68, 2209.

 

(21) Piton, N.; Schiemann, O.; Prisner, T. F.; Engels, J. W.* „Synthesis of Spin-Labeled RNAs for Long Range Distance Measurements by PELDOR” Nucleos. Nucleot. Nucleic. 2005, 24, 771.

 

(22) Lerner, H.-W.; Margraf, G.; Kretz, T.; Schiemann, O.; Bats, J. W.; Dürner, G.; de Biani, F. F.; Zanello, P.; Bolte, M.; Wagner, M.* „Redox Behaviour of Pyrazolyl-Substituted 1,4-Dihydroxyarenes: Formation of the Corresponding Semiquinones, Quinhydrones and Quinones“ Z. Naturforsch. 2006, 61b, 252.

 

(23) Schiemann, O.*; Piton, N.; Plackmeyer, J.; Bode, B. E.; Prisner, T. F.; Engels, J. W.* “Spin labeling of oligonucleotides with the nitroxide TPA and use of PELDOR, a pulse EPR method, to measure intramolecular distances” Nature Protocols 2007, 2, 904 – 923.

 

 

Charge Transfer

(24) Schiemann, O.; Turro, N. J.; Barton, J. K.*  „EPR Detection of Guanine Radicals in a DNA Duplex under Biological Conditions: Selective Base Oxidartion by Ru(phen)₂dppz³⁺ Using the Flash-Quench Technique” J. Phys. Chem. B 2000, 104, 7214.

 

(25) Wan, C; Fiebig, T.; Schiemann, O.; Barton, J. K.; Zewail, A. H.* „Femtosecond direct observation of charge transfer between bases in DNA” Proc. Natl. Acad. Sci. USA, 2000, 97, 14052.

 

(26) Schiemann, O.*; Feresin, E.; Carl, T.; Giese, B „4´-Pivaloyl Substituted Thymidine as a Precursor for the Thymyl Radical. An EPR Spectroscopic Study“ ChemPhysChem 2004, 5, 270.

 

(27) Giese, B.; Carl, B.; Carl, T.; Carell, T.; Behrens, C.; Hennecke, U.; Schiemann, O.*; Feresin, E. „Excess Electron Transport through DNA: A Single Electron Repairs more than One UV-Induced Lesion" Angew. Chem. 2004, 43, 1848.

 

(28) von Feilitzsch, T.; Härter, P.; Schiemann, O.; Michel-Beyerle, M. E.; Steiner, U. E.; Gilch, P.* „Magnetic Field and Temperature Dependencies Shed Light on the Recombination Kinetics of a Transition Metal Donor/Acceptor System” J. Am. Chem. Soc. 2005, 127, 15228.

 

 

Organometallic Molecular Magnets (Research in Marburg)

(29) Elschenbroich, C.*; Schiemann, O.; Burghaus, O.; Harms, K. „Exchange Interaction Mediated by O-H-O Hydrogen Bonds: Synthesis, Structure, and EPR Study of the Paramagnetic Organometallic Carboxylic Acid (h⁷-C₇H₇)V(h⁵-C₅H₄COOH)” J. Am. Chem. Soc. 1997, 119, 7452.

 

(30) Elschenbroich, C.*; Voss, S.; Schiemann, O.; Lippek, A.; Harms, K. „h¹-Coordination of Phosphinine to Chromium, Molybdenum, and Tungsten” Organometallics 1998, 17, 4417.

 

(31) Behrendt, A.; Screttas, C. G.*; Bethell, D.; Schiemann, O.; Steele, B. R. „Magnetic and electrochemical investigations on anions derived from oligoketones containing fluorenone and benzophenone units. An approach to the design of stable multiradical organic materials” J. Chem. Soc., Perkin Trans. 2 1998, 2039.

 

(32) Burghaus, O.’; Schiemann, O.; Wolf, M.; Plackmeyer, J.; Elschenbroich, C. „Determination of Isotropic Exchange Coupling Constants by Simulation Methods” Proceedings of the Joint 29^th AMPERE – 13^th ISMAR 1998, 1186.

 

(33) Elschenbroich, C.*; Schiemann, O.; Burghaus, O.; Harms, K.; Pebler, J. „[5-5]Bitrovacene: Synthesis, Structure, and Intermetallic communication” Organometallics 1999, 18, 3273.

 

(34) Braga, D.*; Maini, L.; Grepioni, F.; Elschenbroich, C.; Paganelli, F.; Schiemann, O.  „Novel Organometallic Building Blocks for Crystal Engeneering. Synthesis and Structural Characterization of the Dicarboxylic Acid [Cr⁰(h⁶-C₆H₅COOH)₂], of Two Polymorphs of it's Oxidation Derivative [Cr^I(h⁶-C₆H₅COOH)₂]⁺[PF6]^-, and of the Zwitterionic Form [Cr^I(h⁶-C₆H₅COO)(h⁶-C₆H₅COOH)]” Organometallics 2001, 20, 1875.

 

(35) Elschenbroich, C.*; Wolf, M.; Schiemann, O.; Harms, K.; Burghaus, O.; Pebler, J. „Head-on versus Side-on [5-5] Bitrovacenes Featuring Benzene and Naphthalene Units as Spacers: How p-Stacking Affects Exchange Coupling and Redox Splitting“ Organometallics 2002, 21, 5810.

 

(36) Schiemann, O.*; Fritscher, J.; Plackmeyer, J.; Elschenbroich, C. “Switching from Spinfrustration to Competing Interactions in Trovacenyl based Organometallic Triradicals“ Appl. Magn. Reson. 2004, 26, 171.

 

(37) Elschenbroich, C*; Schiemann, O.; Burghaus, O; Harms, K. „1,2,4,5-Tetra([5]trovacenyl)benzene: an organometallic tetraradical displaying pronounced electro- and magnetocommunication” Chem. Commun. 2005, 2149.

 

 

Book Contributions

(38) Schiemann, O. „EPR“ in Bioanalytik, Lottspeich, F. und Engels, J. W. (Hrsg.), Spektrum Akademischer Verlag, Heidelberg, 2. Edition, 2006, 441-459.

 

(39) Schiemann, O. „Spin-Labeling“ in Nucleic Acids from A – Z, Müller, S. (Hrsg.), Wiley-VCH, Weinheim, 2008.

 

(40) Schiemann, O. „EPR“ in Nucleic Acids from A – Z, Müller, S. (Hrsg.), Wiley-VCH, Weinheim, 2008.

 

 

Interview

New Scientist: Four artificial new letters for the DNA alphabet (14. July 2008, issue 2664, page 18)

 

C&EN: EPR Sizes Up Histones (26. January 2009, Vol. 87, Nr. 4, p.30)

Publications

Biological EPR-Spectroscopy

 

Centres of Magnetic Resonance and Biomolecular Sciences/Schools of Biology, Physics and Chemistry